An automated technique for double staining rat and rabbit fetal skeletal specimens to differentiate bone and cartilage

Biotechnic and Histochemistry

Volumn 74, Issue 2, 1999, Pages 98-104

  Trueman, Diane ^a   Jackson, Stuart W ^a   Trueman, Brian ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

Alcian blue; Alizarin red; Bone; Cartilage; Double staining; Fetal skeleton; Rabbit; Rat

Indexed keywords

ALCIAN BLUE; ALIZARIN RED S;

ANIMAL TISSUE; ARTICLE; AUTOANALYSIS; BONE; CARTILAGE; FETOTOXICITY; FETUS; FETUS DEVELOPMENT; NONHUMAN; RABBIT; RAT; SKELETON; STAINING;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0032932484     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299909066484     Document Type: Article

References (8)

1. Dawson, A. B. 1926. A note on the staining of the skeleton of cleared specimens with alizarin red S. Stain Technol. 1: 123-124.
2. United States Environmental Protection Agency 1998. Health Effects Test Guidelines OPPTS 870.3700 Prenatal Developmental Toxicity Study.
3. Inouye, M. 1976. Differential staining of cartilage and bone in fetal mouse skeleton by alcian blue and alizarin red S. Congenital Anom. 16: 171-173.
4. Kimmel, C. A. and Trammell, C. 1981. A rapid procedure for routine double staining of cartilage and bone in fetal and adult animals. Stain Technol. 56: 271-273.
5. Miller, D. M. and Tarpley, J. 1996. An automated double staining procedure for bone and cartilage. Biotech. & Histochem. 71: 79-83.
6. Rousseaux, C. G. 1985. Automated differential staining for cartilage and bone in whole mount preparations of vertebrates. Stain Technol. 60: 295-296.
7. Staples, R. E. and Schnell, V. L. 1964. Refinements in rapid clearing technique in the KOH-alizarin red S method for fetal bone. Stain Technol. 39: 61-63.
8. Whitaker, J. and Dix, K. M. 1979. Double staining technique for rat fetus skeletons in teratological studies. Lab. Anim. 13: 309-310.