Multiple biotin-avidin amplification for multiple immunostaining

Applied Immunohistochemistry

Volumn 7, Issue 1, 1999, Pages 73-80

  Ferri, Gian Luca ^a,b,e   Gaudio, Rosa Maria ^a,b,d   Castello, Filippa Ida ^a,b   Tirolo, Cataldo ^a,b   Chiolerio, Francesca ^c  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b OASI RESEARCH INSTITUTE IRCCS   (Italy)

^c Research Department   (Italy)

^d UNIVERSITY OF FERRARA   (Italy)

^e Sez Microbiologia   (Italy)

Author keywords

Avidin; Biotin; Double immunostaining; Fluorochrome; Immunocytochemistry; Immunofluorescence; Immunostaining; Multiple immunostaining

Indexed keywords

AVIDIN; BIOTIN; FLUOROCHROME;

ARTICLE; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE TEST; PRIORITY JOURNAL; STAINING; TISSUE FIXATION;

EID: 0032926230     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199903000-00015     Document Type: Article

References (21)

1. Ali-Rachedi A, Ferri GL, Varndell IM. et al. Immunocytochemical evidence for the presence of gamma 1-MSH-like immunoreactivity in pituitary corticotrophs and ACTH-producing tumours. Neuroendocrinology 1983;37:427-33.
2. Bayer EA, Wilchek M. Protein biotinylation. Methods Enzymol 1990;184:138-60.
3. Bayer E, Skutelsky E, Wilcheck M. The avidin-biotin complex in affinity cytochemistry. Methods Enzymol 1979;62:308-15.
4. Berkenbosch F, Tilders FJH. A quantitative approach to cross-reaction problems in immunocytochemistry. Neuroscience 1987; 23:823-6.
5. Chiolerio F, Filippini E, Magnaghi S, Acerboni R, Malcovati M. Modified avidins as a tool in bridging biotinylated macromolecules. Milan: Chimicaoggi. Edizioni TeknoScienze, 1987:41-8.
6. Ferri GL, Papadia C, Cocchia D, Polak JM. Aluminum foil moulds for cryostat blocks. Stain Technol 1987;62:59-60.
7. Ferri GL, Gaudio RM, Castello IF, Berger P, Giro G. Quadruple immunofluorescence: a direct visualization method. J Histochem Cytochem 1997;45:155-8.
8. Guesdon JL, Ternynck T, Avrameas S. The use of avidin biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 1979;27:1131-9.
9. Haugland RP. Handbook of fluorescent probes and research chemicals, 5th ed. Eugene, OR: Molecular Probes, 1992.
10. Hsu SM. The use of avidin-biotin interaction in immunocytochemistry. In: Cuello AC, ed. Immunocytochemistry II. Chichester, UK: Wiley & Sons, 1993:169-80.
11. Huang WM, Gibson SJ, Facer P, Gu J, Polak JM. Improved section adhesion for immunocytochemistry using high molecular weight polymers of 1-lysine as a slide coating. Histochemistry 1983;77: 275-9.
12. Hunyady B, Krempels K, Harta G, Mezey E. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining. J Histochem Cytochem 1996;44:1353-62.
13. Lan HY, Mu W, Nikolic-Paterson DJ, Atkins RC. A novel, simple, reliable, and sensitive method for multiple immunoenzyme staining: use of microwave oven heating to block antibody crossreactivity and retrieve antigens. J Histochem Cytochem 1995;43:97-102.
14. McLean IW, Nakane PK. Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 1974;22:1077-83.
15. Nielsen MH, Bastholm L, Chatterjee S, Koga J, Norrild B. Simultaneous triple-immunogold staining of virus and host cell antigens with monoclonal antibodies to virus and host cell antigens in ultrathin cryosections. Histochemistry 1989;92:89-93.
16. Pettitt JM, Humphris DC. Double lectin and immunolabelling for transmission electron microscopy: pre-and post-embedding application using the biotin-streptavidin system and colloidal gold-silver staining. Histochem J 1991;23:29-37.
17. Short BG, Zimmerman DM, Schwartz LW. Automated double labeling of proliferation and apoptosis in glutathione S-transferase-positive hepatocytes in rats. J Histochem Cytochem 1997;45:1299-306.
18. Vacca-Galloway LL. Differential immunostaining for substance P in Huntington's diseased and normal spinal cord: significance of serial (optimal, supra-optimal and end-point) dilutions of primary anti-serum in comparing biological specimens. Histochemistry 1985;83:561-9.
19. van Gijlswijk RP, Zijlmans HJ, Wiegant J, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem 1997;45:375-82.
20. Wang BL, Larsson LI. Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species. Histochemistry 1985;83:47-56.
21. Wessendorf MW, Breljie TC. Which fluorophore is brightest. A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas red, and cyanine 3.18. Histochemistry 1992;98:81-5.