Quantitative assessment of human leukocyte antigen-G protein in amniotic fluid by a double-determinant enzyme-linked immunosorbent assay using anti-human leukocyte antigen-G-specific antibody '87G'

American Journal of Reproductive Immunology

Volumn 41, Issue 4, 1999, Pages 293-295

  Hamai, Yoko ^a   Fujii, Tomoyuki ^a   Miki, Akinori ^a   Geraghty, Daniel E ^b   Harada, Ikuko ^a   Takai, Yasushi ^a   Kozuma, Shiro ^a   Tsutsumi, Osamu ^a   Taketani, Yuji ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b Fred Hutchinson Cancer Research Center   (United States)

Author keywords

Amniotic fluid; Enzyme linked immunosorbent assay; Human leukocyte antigen G; Pregnancy; Trophoblast

Indexed keywords

BETA 2 MICROGLOBULIN; HLA ANTIBODY; HLA G ANTIGEN; HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY;

ADULT; AMNION FLUID; ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DEVICE; ENZYME LINKED IMMUNOSORBENT ASSAY; EQUIPMENT; FEMALE; HUMAN; HUMAN CELL; IMMUNOASSAY; MALE; MILK; PRIORITY JOURNAL; SECOND TRIMESTER PREGNANCY;

EID: 0032923422     PISSN: 10467408     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0897.1999.tb00441.x     Document Type: Article

References (7)

1. Geraghty DE, Koller BH, Orr TH: A human major histocompatibility complex class I gene that encodes a protein with a shortened cytoplasmic segment. Proc Natl Acad Sci USA 1987; 84:9145-9199.
2. Kovats S, Main EK, Librach C, Stubblebine M, Fisher SJ, DeMars R: A class I antigen, HLA-G, expressed in human trophoblasts. Science 1990; 248:220-223.
3. Fujii T, Ishitani A, Geraghty DE: A soluble form of the HLA-G antigen is encoded by a messenger ribonucleic acid containing intron 4. J Immunol 1994; 153:5516-5524.
4. Hammer AH, Blaschitz A, Mahnert W, Hartmann M, Uchanska-Ziegler B, Ziegler A, Dohr G: Amnion epithelial cells, in contrast to trophoblast cells, express all classical HLA class I molecules together with HLA-G. Am J Reprod Immunol 1997; 37:161-171.
5. Lee N, Malacko AR, Ishitani A, Chen MC, Bajorath J, Marquardt H, Geraghty DE: The membrane-bound and soluble forms of HLA-G bind identical sets of endogenous peptides but differ with respect to TAP association. Immunity 1995; 3:591-600.
6. Hornbeck P: Double antibody-sandwich ELISA to detect soluble antigens. In Current Protocols in Molecular Biology, F Ausubel, R Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, K Struhl (eds). New York, WileyLiss, 1993, pp. 11.2.10-11.2.13.
7. Hara N, Fujii T, Yamashita T, Kozuma S, Okai T, Taketani Y: Altered expression of human leukocyte antigen G (HLA-G) on extravillous trophoblasts in preeclampsia: Immunohistological demonstration with anti-HLA-G specific antibody "87G" and anticytokeratin antibody "CAMS.2". Am J Reprod Immunol 1996; 36:349-358.