The Neocallimastix patriciarum cellulase, CelD, contains three almost identical catalytic domains with high specific activities on Avicel

Enzyme and Microbial Technology

Volumn 24, Issue 8-9, 1999, Pages 609-614

  Aylward, James H ^a   Gobius, Kari S ^a   Xue, Gang Ping ^a   Simpson, Graham D ^a   Dalrymple, Brian P ^a  

^a CSIRO   (Australia)

Author keywords

Cellulase; Endoglucanase; Neocallimastix patriciarum; Rumen; Trichoderma reseei

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYSIS; CATALYST ACTIVITY; DNA SEQUENCES; ENZYME KINETICS; ENZYMES; HYDROLYSIS; PROTEINS;

AVICEL; CELLULASE; ENDOGLUCANASE; GENETIC MANIPULATION; NEOCALLIMASTIX PATRICIARUM; RUMEN; TRICHODERMA REESEI;

CLONING;

CELLULASE; COMPLEMENTARY DNA;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; FUNGUS; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; TRICHODERMA REESEI;

ESCHERICHIA COLI; NEOCALLIMASTIX PATRICIARUM; TRICHODERMA REESEI;

EID: 0032921044     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00167-7     Document Type: Article

References (25)

1. Orpin, C. G. Studies on the rumen flagellate. Neocallimastix frontalis. J. Gen. Microbiol. 1975, 91, 249-262.
2. Orpin, C. G. and Munn, E. A. Neocallimastix patriciarum sp. nov., a new member of the Neocallimasticaceae inhabiting the rumen of sheep. Trans. Br. Mycol. Soc. 1986, 86, 178-180
3. Wood, T. M., Wilson, C. A., McCrae, S. I., and Joblin, K. N. A highly active cellulase from the rumen anaerobic fungus Neocallimastix frontalis. FEMS Microbiol. Lett. 1986, 34, 37-40
4. Williams, A. G. and Orpin, C. G. Polysaccharide-degrading enzymes formed by three species of anaerobic rumen fungi grown on a range of carbohydrate substrates. Can. J. Microbiol. 1987, 33, 418-426
5. Flint, H. J. and Wallace, R. J. Manipulating rumen microorganisms and rumen fermentation. In: Annual Report. Rowett Research Institute, Aberdeen, UK 1991, 38-43
6. Johnsson, I. D. The Australian meet research corporation's rumen bacteria manipulation key program - rationale and progress 1991-1995. In: Proceedings of a Workshop on Rumen Ecology Research Planning (Wallace, R. J. and Lahlou-Kassi, A., Eds.). ILRI, Addis Ababa, Ethiopia, 1995, 55-61
7. Aylward, J. H. Biotechnology of ruminant nutrition. In: Biennial Research Report 1992-94, CSIRO Division of Tropical Crops and Pastures, Brisbane, Australia, 1995, 131-134
8. Xue, G.-P., Orpin, C. G., Gobius, K. S., Aylward, J. H., and Simpson, G. D. Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli. J. Gen. Microbiol. 1992, 138, 1413-1420
9. Xue, G.-P., Gobius, K. S., and Orpin, C. G. A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multifunctional catalytic domains with high endoglucanase, cellobiohydrolase and xylanase activities. J. Gen. Microbiol. 1992, 138, 2397-2403
10. Gilbert, H. J., Hazlewood, G. P., Laurie, J. I., Orpin, C. G., and Xue, G. P. Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin. Mol. Microbiol. 1992, 6, 2065-2072
11. Dalrymple, B. P., Cybinski, D. H., Layton, I., McSweeney, C. S., Xue, G.-P., Swadling, Y. L., and Lowry, J. B. Three Neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases. Microbiology 1997, 143, 2605-2614
12. Orpin, C. G. and Xue, G.-P. Genetics of fibre degradation in the rumen, particularly in relation to anaerobic fungi, and its modification by recombinant DNA technology. Proceedings of the XVII International Grassland Congress, 1993, 2, 1209-1214
13. Xue, G. P., Johnson, J. S., Bransgrove, K. L., Gregg, K., Beard, C. E., Dalrymple, B. P., Gobius K. S., and Aylward, J. H. Improvement of expression and secretion of a fungal xylanase in the rumen bacterium Butyrivibrio fibrisolvens OB156 by manipulation of promoter and signal sequences. J. Biotechnol. 1997, 54, 139-148
14. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning 2nd Ed. Cold Spring Harbor Laboratory Press, USA, 1989
15. Denman, S., Xue, G.-P., and Patel, B. Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei cellobiohydrolase II. Appl. Environ. Microbiol. 1996, 62, 1889-1896
16. Sanger, F., Nicklen, S., and Coulson, A. R. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 1977, 74, 5463-5467
17. Wood, T. M. and Bhat, K. M. Methods for measuring cellulase activities. Meth. Enzymol. 1988, 160, 87-112
18. Lever, M. A new reaction for colorimetric determination of carbohydrates. Anal. Biochem. 1972, 47, 273-279
19. Schagger, H. and Von Jagow, G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 1987, 166, 368-379
20. Merril, C. R., Goldman, D., Sedman, S. A., and Ebert, M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 1981, 211, 1437-1438
21. Zhou, L., Xue, G. P., Orpin, C. G., Black, G. W., Gilbert, H. J., and Hazlewood, G. P. Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase. Biochem. J. 1994, 297, 359-364
22. Fanutti, C, Ponyi, T., Black, G. W., Hazlewood, G. P., and Gilbert, H. J. The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. J. Biol. Chem. 1995, 270, 29314-29322
23. Walker, L. P. and Wilson, D. B. Enzymatic hydrolysis of cellulose. Biores. Technol. 1991, 36, 3-14
24. Huang, A. A. Kinetic studies on insoluble cellulose-cellulase system. Biotechnol. Bioeng. 1975, 17, 1421-1433
25. Lee, Y. H. and Fan, L. T. Kinetic studies of enzymatic hydrolysis of insoluble cellulose: (II). Analysis of extended hydrolysis time. Biotechnol. Bioeng. 1982, 24, 2383-2406