Mouse C127 cells transfected with fucosyltransferase Fuc-TIII express masked Lewis(x) but not Lewis(x) antigen

Glycobiology

Volumn 9, Issue 1, 1999, Pages 83-91

  Valli, Maurizia ^a   Bardoni, Anna ^a   Trinchera, Marco ^a,b  

^a UNIVERSITY OF PAVIA   (Italy)

^b Dipartimento di Biochimica   (Italy)

Author keywords

Carbohydrate antigens; Glycosyltransferase; Lectins; Western blot

Indexed keywords

ALPHA GALACTOSIDASE; COMPLEMENTARY DNA; FUCOSYLTRANSFERASE; GLYCAN; GLYCOPROTEIN; OLIGONUCLEOTIDE; PRIMER RNA; RNA; SIALIDASE; VIRUS T ANTIGEN;

ANIMAL CELL; ARTICLE; BLOOD GROUP LEWIS SYSTEM; CELL CLONE; CONTROLLED STUDY; DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME KINETICS; FLOW CYTOMETRY; GENE AMPLIFICATION; GENETIC TRANSFECTION; HUMAN; IMMUNOBLOTTING; LECTIN BINDING; MOUSE; NONHUMAN; POLYOMA VIRUS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; ANTIGENS, CD15; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CONCANAVALIN A; COS CELLS; CRICETINAE; FLOW CYTOMETRY; FUCOSE; FUCOSYLTRANSFERASES; HUMANS; LECTINS; MAMMARY NEOPLASMS, EXPERIMENTAL; MICE; OLIGOSACCHARIDES; TRANSFECTION; TUMOR CELLS, CULTURED;

ANIMALIA; GRIFFONIA SIMPLICIFOLIA; POLYOMAVIRUS; SAMBUCUS NIGRA;

EID: 0032917141     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/9.1.83     Document Type: Article

References (39)

1. Blanken, W.M. and Van den Eijnden, D.H. (1985) Biosynthesis of terminal Galα1,3Galβ1,4GlcNAc-R oligosaccharide sequences on glycoconjugates. J. Biol. Chem., 260, 12927-12934.
2. Bry, L., Falk, P.G. and Gordon, J.I. (1996) Genetic engineering of carbohydrate biosynthetic pathways in transgenic mice demonstrates cell cycle-associated regulation of glycoconjugate production in small intestinal epithelial cells. Proc. Natl. Acad. Sci. USA, 93, 1161-1166.
3. Chandrasekaran, E.V., Jain, R.K., Rhodes, J.M., Srnka, C.A., Larsen, R.D. and Matta, K.L. (1995) Expression of blood group Lewis b deteminant from Lewis a: association of this novel α(1,2)-L-fucosylating activity with the Lewis type α(1,3/4)-1-fucosyltransferase. Biochemistry, 34, 4748-4756.
4. Chen, C. and Okayama, H. (1987) High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell Biol., 7, 2745-2752.
5. Cho, S.K. Yeh, J., Cho, M. and Cummings, R.D. (1996) Transcriptional regulation of α1,3galactosyltransferase in embryonal carcinoma cells by retinoic acid. J. Biol. Chem., 271, 3238-3246.
6. Costa, J., Grabenhorst, E., Nimtz, M. and Conrad, H.S. (1997) Stable expression of the Golgi form and secretory variants of human fucosyltransferase III from BHK-21 cells, J. Biol. Chem., 272, 11613-11621.
7. Costache, M., Apoil, P.A., Cailleau, A., Elmgren, A., Larson, G., Henry, S., Blancher, A., Iordachescu, D., Oriol, R., and Mollicone, R. (1997) Evolution of fucosyltransferase genes in vertebrate. J. Biol. Chem., 272, 29721-29728.
8. Cummings, R.D. (1994a) Structural characterization of N-glycans obtained from metabolically radiolabeled glycoproteins. In Fukuda, M. and Kobata, A. (eds.), Glycohiology - A Pratical Approach. IRL Press, Oxford, pp. 243-289.
9. Cummings, R.D. (1994b) Use of lectin in analysis of glycoconjugates. Methods Enzymol., 230, 66-86.
10. de Vries, T, Srnka, C.A., Palcic, M.M., Swiedler, S.J., Van den Eijnden, D.H. and Macher, B.A. (1995) Acceptor specificity of different constructs of human recombinant α1,3/4fucosyltransferases. J. Biol. Chem., 270, 8712-8722.
11. Elmgren, A.. Mollicone, R., Costache, M., Borjeson, C., Oriol, R., Harrington, R. and Larson, G (1997) Significance of individual point mutation, T202C and C314T. in the human Lewis (FUT3) gene for expression of Lewis antigens by the human alpha (1,3/1,4)-fucosyltransferase. Fuc-TIII. J. Biol. Chem., 272, 21994-21998.
12. Furukawa, K., Funakoshi, Y., Autero, M., Horejsi, V., Kobata, A. and Gahmberg, C.G. (1998) Structural study of the O-linked sugar chains of human leukocyte tyrosine phosphatase CD45. Eur. J. Biochem., 251, 288-294.
13. Gersten, K.M., Natsuka, S., Trinchera, M., Petryniac. B., Kelly, R.J.. Hiraiwa, N., Jenkins, N.A., Gilbert, D.J., Copeland.N.G. and Lowe, J.B. (1995) Molecular cloning, expression, chromosomal assignment, and tissue-specific expression of a murine α-(1,3)-fucosyltransferase locus corresponding to the human ELAM-1 ligand fucosyl transferase. J Biol Chem., 270, 25047-25056.
14. x) by two Chinese hamster ovary cell lines transfected with the same α1,3fucosyltransferase gene (ELFT). J. Biol. Chem., 269, 1033-1040.
15. Gowda, D.C. and Davidson, E.A. (1994) Isolation and characterization of novel mucin-like glycoproteins from cobra venom. J. Biol. Chem., 269, 20031-20039.
16. Hartman, S.C. and Mulligan, R.C. (1988) Two dominant-acting selectable markers for gene transfer studies in mammalian cells. Proc. Natl. Acad. Sci. USA, 85, 8047-8051.
17. Hiraiwa, N., Hi, T., Kawakami-Kimura, N., Yumen, M., Ohmori, K., Maeda, M. and Kannagi, R. (1996) Suppression of sialyl-Lewis x expression and E-selectin-mediated cell adhesion in cultured human lymphoid cells by transfection of antisense cDNA of an α1,3fucosyltransferase (Fuc-T VII). J. Biol. Chem., 271, 31556-31561.
18. Kagawa, Y., Takasaki, S., Utsumi, J., Osoi, K., Shimizu, H., Kochibel, N. and Kobata, A. (1988) Comparative study of the asparagine-linked sugar chains of natural human interferon-β1 produced by three different mammalian cells. J. Biol. Chem., 263, 17508-17515.
19. Kukowska-Latallo, J.F., Larsen, R.D., Nair, R.P. and Lowe, J.B. (1990) A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group α(1,3/1.4)fucosyltransferase. Genes Dev., 4, 1288-1303.
20. Joziasse, D.H., Schiphorst, W.E.C.M., Koeleman, C.A.M. and Van den Eijnden, D.H. (1993) Enzymatic synthesis of the alpha 3-galactosyl-Lex tetrasaccharide: a potential ligand for selectin-type adhesion molecules. Biochem. Biophvs. Res. Commun., 194, 358-367.
21. Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
22. Larsen, R.D., Ernst, L.K., Nair, R.P. and Lowe, J.B. (1990) Molecular cloning, sequence, and expression of a human GDP-L-fucose: β-D-galactoside 2-α-L-fucosyltransferase cDNA that can form the H blood group antigen. Proc. Natl. Acad. Sci. USA, 87, 6674-6678.
23. Legault, D.J, Kelly, R.J., Natsuka, Y. and Lowe, J.B. (1995) Human αl,3/4fucosyltransferases discriminate between different oligosaccharides acceptor substrates through a discrete peptide fragment. J. Biol. Chem., 270, 20987-20996.
24. Lowe, J.B., Stoolman, L.M., Nair, R.P., Larsen, R.D., Berhend, T.L. and Marks, R.M. (1990) ELAM-1 dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA. Cell, 63, 475-484.
25. Magnani, J.L., Spitalnik, S.L. and Ginsburg, V. (1987) Antibodies against cell surface carbohydrates: determination of antigen structure. Methods Enzvmol., 138, 195-207.
26. Majuri, M.-L., Niemel S,R., Tiisala, S., Renkonen, O. and Renkonen, R. (1995) Expression and function of α2,3sialyl- and α1,3/1,4fucosyltransferases in colon adenocarcinoma cell lnes: role in synthesis of E-selectin counter receptors. Int. J. Cancer, 63, 551-559.
27. Merkle, R.K. and Cummings, R.D. (1987) Relationship of the terminal sequences to the length of poly-N-acetylactosamine chains in asparagine-linked oligosaccharides from the mouse limphoma cell line BW5147. J. Biol. Chem., 262, 8179-8189.
28. Natzuka, S. and Lowe, J.B. (1994) Enzymes involved in mammalian oligosaccharide biosynthesis. Curr. Opin. Struct. Biol., 4, 683-691.
29. Pennington, J.E., Rastan, S., Roelcke, D. and Feizi, T. (1985) Saccharide structures of mouse embryo during the first eight days of development. J. Embryol., 90, 335-361.
30. Sarnesto, A., Kohlin, T., Hindsgaul, O., Thurin, J. and Blaszczyk-Thurin, M. (1992) Purification of secretor type β-galactoside α1,2fucosyltransferase from human serum. J. Biol. Chem., 267, 2737-2744.
31. Smith, D.F., Larsen, R.D., Mattox, S., Lowe, J.B. and Cummings, R.D. (1990) Transfer and expression of murine UDP-Gal: β-D-Gal- α1 ,3galactosyltransferase gene in transfected chinese hamster ovary cells. J. Biol. Chem., 265, 6225-6234.
32. Smith, P.L. and Lowe, J.B. (1994) Molecular cloning of a murine N-acetylgalactosamine-transferase cDNA that determines expression of the T lymphocyte-specific CT oligosaccharide differentiation antigen. J. Biol. Chem., 269, 15162-15171.
33. Sueyoshi, S., Tsuboi, S., Sawada-Hirai, R., Dang, U.N., Lowe, J.B. and Fukuda, M. (1994) Expression of a distinct fucosylated oligosaccharides and carbohydrate mediated adhesion efficiency directed by two different α1,3fucosyltransferases. J. Biol. Chem., 269, 32342-32350.
34. Towbin, H., Stachlin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA, 76, 4350-4354.
35. Trinchera, M. and Bozzaro, S. (1996) Dyctiostelium cytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro. FEBS Lett., 395, 68-72.
36. Trinchera, M., Carrettoni, D. and Ghidoni, R. (1991) A part of glucosylceramide formed from exogenous lactosylceramide is not degraded to ceramide but re-cycled and glycosylated in the Golgi apparatus. J. Biol. Chem., 266, 9093-9099.
37. Weston, B.W., Nair, R.P., Larsen, R.D. and Lowe, J.B. (1992) Isolation of a novel human α1,3fucosyltransferase gene and molecular comparison to the human Lewis blood group α1,3/1,4fucosyltransferase. J. Biol. Chem., 267, 4152-4160.
38. a, the carbohydrate ligands for E- and P-selectins, in human malignant cell lines. Cancer Res., 53, 5559-5565.
39. Zollner, O. and Vestweber, D. (1996) The E-selectin ligand-1 is selectively activiated in chinese hamster ovary cells by the α1,3fucosyltransferases IV and VII. J. Biol. Chem., 271, 33002-33008.