Rapid colorimetric assay of urinary β-galactosidase and N-acetyl-β-D- glucosaminidase with Cobas Mire Auto-analyzer

Journal of Clinical Laboratory Analysis

Volumn 13, Issue 3, 1999, Pages 95-98

  Xu, Guobing ^a   Zhu, Lihua ^a   Hong, Jianmei ^a   Cao, Yi ^a   Xia, Tiean ^a  

^a First Teaching Hospital   (China)

Author keywords

galactosidase; Automatic assay; N acetyl D glucosaminidase; Urine

Indexed keywords

BETA GALACTOSIDASE; N ACETYL BETA GLUCOSAMINIDASE;

ADOLESCENT; ADULT; ARTICLE; BRUSH BORDER; CHILD; COLORIMETRY; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME LOCALIZATION; ENZYME MECHANISM; ENZYMURIA; FEMALE; HUMAN; INCUBATION TIME; INFANT; INTERMETHOD COMPARISON; IONIC STRENGTH; KIDNEY INJURY; KIDNEY PROXIMAL TUBULE; MAJOR CLINICAL STUDY; MALE; PH; PROTEIN URINE LEVEL;

ACETYLGLUCOSAMINIDASE; AUTOANALYSIS; BETA-GALACTOSIDASE; BUFFERS; COLORIMETRY; HUMANS; HYDROGEN-ION CONCENTRATION; OSMOLAR CONCENTRATION; REFERENCE VALUES; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 0032916610     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1999)13:3<95::AID-JCLA1>3.0.CO;2-L     Document Type: Article

References (6)

1. Dance N, Price RG, Robinson D, et al. β-glucosidase and N-acetyl-β-D-glucosaminidase in human kidney. Clin Chim Acta 1969;24: 189-197.
2. Makise J, Saito E, Obuchi M, et al. Kinetic rate assay of urinary N-acetyl-β-D-glucosaminidase with 2-chloro-4-nitrophenyl-N-acetyl-β-D-glucosaminide as substrate. Clin Chem 1988;34:2140-2143.
3. Hu Z-Y, Wei Y-R, Hu Z-H, et al. A sensitive kinetic assay for β-D-galactoside (GAL) with chloro-nitrophenyl-β-D-galactoside as substrate (abstract). Clin Chem 1990;36:1136.
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5. Maruhn D, Paar D. Urinary enzymes in therapy monitoring and recognition of side effects of drugs. In: Burlina A, Galzigna L, editors. Clinical enzymology symposia: proceedings of the 9th international symposium on clinical enzymology held in Verona, April 17-20th, 1980. Padua & London: Piccini Medical Books; 1982. p 137-152.
6. Dance N, Price RG, Cattell WR, et al. The excretion of N-acetyl-β-D-glucosaminidase and β-galactosidase by patients with renal disease. Clin Chim Acta 1970;27:87-92.