Identification and isolation of differentially expressed genes from very small tissue samples

BioTechniques

Volumn 26, Issue 5, 1999, Pages 884-892

  Gonzalez, Pedro ^a,c   Zigler Jr , J Samuel ^a   Epstein, David L ^b   Borras, Teresa ^b  

^a DUKE UNIVERSITY   (United States)

^b NATIONAL EYE INSTITUTE   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DIAGNOSTIC APPROACH ROUTE; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; GENE ISOLATION; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOLATION PROCEDURE; MOLECULAR BIOLOGY; NUCLEOTIDE SEQUENCE; SAMPLING; VALIDATION PROCESS;

EID: 0032914156     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99265st01     Document Type: Article

References (15)

1. Bernard, K., N. Auphan, S. Granjeaud, G. Victorero, A.M. Schmitt-Verhulst, B.R. Jordan and C. Nguyen. 1996. Multiplex messenger assay: simultaneous, quantitative measurement of expression of many genes in the context of T cell activation. Nucleic Acids Res. 24:1435-1442.
2. Borson, N.D., W.L. Salo and L.R. Drewes. 1992. A lock-docking oligo(dT) primer for 5′ and 3′ RACE PCR. Genome Res. 2:144-148.
3. Clark, J.M., C.M. Joyce and G.P. Beardsley. 1987. Novel blunt-end addition reactions catalyzed by DMA polymerase I of Escherichla coli. J. Mol. Biol. 795:123-127.
4. DeRisi, J.L., V.R. lyer and P.O. Brown. 1997. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 275:680-686.
5. DeRisi, J., L. Penland, P.O. Brown, M.L. Bittner, P.S. Meltzer, M. Ray, Y. Chen, Y.A. Su and J.M. Trent. 1996. Use of a cDNA microarray to analyze gene expression patterns in human cancer. Nature Genet. 74:457-460.
6. Feinberg, A.P. and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 752:6-13.
7. Horstmann, H.J., J.W. Rohen and K. Samcs. 1983. Age-related changes in the composition of proteins in the trabecular meshwork of the human eye. Mech. Ageing Dev. 27:121-136.
8. Jordan, B.R. 1998. Large-scale expression measurement by hybridization methods: from high density membranes to "DNA chips". J. Biochem. 724:251-258.
9. Lennon, G., C. Auffray, M. Polymeropoulos and M.B. Soarcs. 1996. The I.M.A.G.E. consortium: an integrated molecular analysis of genomes and their expression. Genomics 55:151-152.
10. Nguyen, C., D. Rocha, S. Granjeaud, M. Baldit, K. Bernard, P. Naquet and B.R. Jordan. 1995. Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cDNA clones. Genomics 29:207-216.
11. Patel, P.H. and B.D. Preston. 1994. Marked infidelity of human immunodeficiency virus type 1 reverse transcriptase at RNA and DNA template ends. Proc. Natl. Acad. Sei. USA 97:549-553.
12. Pietu, G., O. Alibert, V. Guichard, B. Lamy, F. Bois, E. Leroy, R. Mariage-Sampson, R. Houlgatte, P. Soularue and C. Auffray. 1996. Novel gene transcripts preferentially expressed in human muscles revealed by quantitative hybridization of a high density cDNA array. Genome Res. 0:492-503.
13. Schena, M., D. Shalon, R.W. Davis and P.O. Brown. 1995. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270:467-470.
14. Schena, M., D. Shalon, R. Heller, A. Chai, P.O. Brown and R.W. Davis. 1996. Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. Proc. Nat. Acad. Sei. USA 95:10614-10619.
15. Stamer, W.D., R.E. Seftor, S.K. Williams, H.A. Samaha and R.W. Snyder. 1995. Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion. Curr. Eye Res. 74:611-617.