Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels

Electrophoresis

Volumn 20, Issue 3, 1999, Pages 483-485

  Suresh, Cuddapah ^a   Dubey, Ashok K ^a   Kini, Ramachandra ^c   Umesh Kumar, Sukumaran ^a   Karanth, Naikantatte G ^a,b  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

^b Dept Ferment Technol and Bioeng ^*  (India)

^c UNIVERSITY OF MYSORE   (India)

Author keywords

Direct detection; Glucoamylase; Isoelectric focusing; Raw starch hydrolysis

Indexed keywords

CHLORINE COMPOUNDS; ELECTROPHORESIS; FOCUSING; GELS; HYDROLYSIS; STARCH;

DIRECT-DETECTION; GELATINIZED STARCH; GLUCOAMYLASE; HYDROLYZING ACTIVITY; ISOELECTRIC FOCUSING; RAW STARCH; RAW STARCH HYDROLYSIS; REACTION PRODUCTIONS; SEPARATION DETECTIONS; TRIPHENYLTETRAZOLIUM CHLORIDES;

ASPERGILLUS;

GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE; STARCH; TRIPHENYLTETRAZOLIUM;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; HYDROLYSIS; IMMUNOBLOTTING; ISOELECTRIC FOCUSING;

EID: 0032913653     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(19990301)20:3<483::AID-ELPS483>3.0.CO;2-B     Document Type: Article

References (16)

1. Boel, E., Hansen, M. T., Hjort, I., Hoegh, I., Fill, N. P., EMBO J. 1984, 3, 1581-1585.
2. Ueda, S., Trends Biochem. Sci. 1981, 6, 89-90.
3. Svensson, B., Larsen, K., Gunnarsson, A., Eur. J. Biochem. 1986, 154, 497-502.
4. Saha, B. C., Zeikus, J. G., Stärke 1989, 41, 57-64.
5. Belshaw, N. J., Williamson, G., FEBS Lett. 1990, 269, 350-353.
6. Stoffer, B., Frandsen, T. P., Busk, P. K., Schneider, P., Svendsen, I., Svensson, B., Biochem. J. 1993, 292, 197-202.
7. Frendsen, P. T., Christensen, T., Stoffer, B., Lehmbeck, J., Dupont, C., Hozatko, R. B., Svensson, B., Biochemistry 1995, 34, 10162-10169.
8. Ueda, S., Saha, B. C., Stärke 1980, 32, 420-423.
9. Gabriel, O., Wang, S. F., Anal. Biochem. 1969, 27, 545-554.
10. Mukasa, H., Shimamura, A., Tsumori, H., Anal. Biochem. 1982, 123, 276-284.
11. Laemmli, U. K., Nature 1970, 227, 680-685.
12. Morrissey, J. H., Anal. Biochem. 1981, 117, 307-310.
13. Pan, S., Ye, X., Kuc, J., Anal. Biochem. 1989, 182, 136-140.
14. Suresh, C., Dubey, A. K., Kavitha, R., Umesh-Kumar, S., Anal. Biochem. 1998, 264, 286-288.
15. Paterson, R. R. M., Bridge, P. D., Crosswaite, M. J., Hawkworth, D. Z., J. Gen. Microbiol. 1989, 135, 2979-2991.
16. Spector, T., Anal. Biochem. 1978, 86, 142-146.