n-Butanol purification of dye terminator sequencing reactions

BioTechniques

Volumn 26, Issue 4, 1999, Pages 606-610

  Tillett, Daniel ^a   Neilan, Brett A ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; BUTANOL;

AUTOMATION; DNA PURIFICATION; DNA SEQUENCE; SHORT SURVEY; STAINING;

EID: 0032911894     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99264bm02     Document Type: Short Survey

References (5)

1. Dittmann, E., B.A. Neilan, M. Erhard, H. von Döhren and T. Börner. 1997. Insertional mutagenesis of a peptide synthetase gene that is responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806. Mol. Microbiol. 20:779-787.
2. Fleischmann, R.D., M.D. Adams, O. White, R.A. Clayton, E.F. Kirkness, A.R. Kerlavage, CJ. Bult, J.F. Tomb, B.A. Dougherty, J.M. Merrick, K. McKenney et al. 1995. Whole-genome random sequencing and assembly of Haemophilus injluenzae. Science 269:496-512.
3. 3.PE Applied Biosystems. 1997. ABI Prism™ BigDye™ Terminator Cycle Sequence Ready Reaction Kit Protocol. Foster City, CA.
4. Sawadogo, M. and M.W. Van Dyke. 1991. A rapid method for the purification of deprotect-ed oligodeoxynucleotides. Nucleic Acids Res. 79:674.
5. Thomas, M.R. 1994. Simple, effective cleanup of DNA ligation reactions prior to electro-transformation of E. coli. BioTechniques 16:988-990.