Genetic evidence for Pak1 autoinhibition and its release by Cdc42

Molecular and Cellular Biology

Volumn 19, Issue 1, 1999, Pages 602-611

  Tu, Hua ^a,b,c   Wigler, Mike ^a  

^a Simons Center for Quantitative Biology   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE; PROTEIN KINASE;

ARTICLE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME RELEASE; MOLECULAR INTERACTION; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEX DIFFERENTIATION; SIGNAL TRANSDUCTION;

SCHIZOSACCHAROMYCES POMBE;

EID: 0032907949     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.19.1.602     Document Type: Article

References (41)

1. Bagrodia, S., B. Derijard, R. J. Davis, and R. A. Cerione. 1995. Cdc42 and PAK-mediated signaling leads to JNK and p38 mitogen-activated protein kinase activation. J. Biol. Chem. 270:1-4.
2. Barr, M. M., H. Tu, L. Van Aelst, and M. Wigler. 1996. Identification of Ste4 as a potential regulator of Byr2 in the sexual response pathway of Schizo-saccharomyces pombe. Mol. Cell. Biol. 16:5597-5603.
3. Brown, J., L. Stowers, M. Baer, A. Trejo, S. Coughlin, and J. Chant. 1996. Human STE20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway. Curr. Biol. 6:598-605.
4. Burbelo, P. D., D. Drechsel, and A. Hall. 1995. A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases. J. Biol. Chem. 270:29071-29074.
5. Chang, E. C., M. Barr, Y. Wang, V. Jung, H.-P. Xu, and M. Wigler. 1994. Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Cell 79:131-141.
6. Coso, O. A., M. Chiariello, J. C. Yu, H. Teramoto, P. Crespo, N. Xu, T. Miki, and J. S. Gutkind. 1995. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway. Cell 81:1137-1146.
7. Cvrckova, F., C. De Virgilio, E. Manser, J. Pringle, and K. Nasmyth. 1995. Set20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeasts. Genes Dev. 6:1817-1830.
8. Fields, S., and O.-K. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-246.
9. Gilbreth, M., P. Yang, D. Wang, J. Frost, A. Polverino, M. H. Cobb, and S. Marcus. 1998. The highly conserved skb1 gene encodes a protein that interacts with SHK1, a fission yeast Ste20/PAK homolog. Proc. Natl. Acad. Sci. USA 93:13802-13807.
10. Hagen, D., G. McCaffrey, and G. Sprague. 1991. Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 11: 2952-2961.
11. Hill, C. S., J. Wynne, and R. Treisman. 1995. The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF. Cell 81:1159-1170.
12. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
13. Leberer, E., D. Dignar, D. Harcus, D. Thomas, and M. Whiteway. 1992. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein β subunits to downstream signalling components. EMBO J. 11:4815-4824.
14. Leberer, E., D. Dignard, L. Hougan, D. Y. Thomas, and M. Whiteway. 1992. Dominant-negative mutants of yeast G-protein β subunit identify two functional regions involved in pheromone signaling. EMBO J. 11:4805-4813.
15. Leevers, S. J., H. F. Paterson, and C. J. Marshall. 1994. Requirement for Ras in Raf activation is overcome by targeting Raf to the plasma membrane. Nature 369:411-418.
16. Manser, E., T. Leung, H. Salihuddin, Z. S. Zhao, and L. Lim. 1994. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 367: 40-46.
17. Marcus, S., A. Polverino, E. Chang, D. Robbins, M. H. Cobb, and M. Wigler. 1995. Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65pak protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast, Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 92: 6180-6184.
18. Marcus, S., A. Polverino, M. Barr, and M. Wigler. 1994. Complexes between STE5 and components of the yeast pheromone-responsive MAP kinase module. Proc. Natl. Acad. Sci. USA 91:7762-7766.
19. Marshall, C. 1994. MAP kinase kinase kinase, MAP kinase kinase, and MAP kinase Curr. Opin. Genet. Dev. 4:82-89.
20. Martin, H., A. Mendoza, J. M. Rodriguez-Pachon, M. Molina, and C. Nombela. 1997. Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase. Mol. Microbiol. 23:431-444.
21. Maser, E., C. Chong, Z. S. Zhao, T. Leung, G. Michael, C. Hall, and L. Lim. 1998. Molecular cloning of a new member of the p21-Cdc42/Rac-activated kinase (PAK) family. J. Biol. Chem. 2740:25070-25078.
22. Masuda, T., K.-C. Kariya, M. Shinkai, T. Okada, and T. Kataoka. 1995. Protein kinase Byr2 is a target of Ras1 in the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 270:1979-1982.
23. Minden, A., A. Lin, F. X. Claret, A. Abo, and M. Karin. 1995. Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs. Cell 81:1147-1157.
24. Mullis, K. B., and F. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335-350.
25. Neiman, A., and I. Herskowitz. 1994. Reconstitution of a yeast protein kinase cascade in vitro: activation of the yeast MEKK homologue STE7 by STE11. Proc. Natl. Acad. Sci. USA 91:3398-3402.
26. Ottilie, S., P. J. Miller, D. I. Johnson, C. L. Creasy, M. A. Sells, S. Bagrodia, S. L. Forsburg, and J. Chernoff. 1995. Fission yeast pak1 + encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating. EMBO J. 14:5908-5919.
27. Polverino, A., J. Frost, P. Yang, M. Hutchison, A. Neiman, M. Cobb, and S. Marcus. 1995. Activation of mitogen-activated protein kinase cascade by p21 -activated protein kinase in cell-free extracts of Xenopus oocytes. J. Biol. Chem. 270:26067-26070.
28. Ramer, S. W., and R. W. Davis. 1993. A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90:452-456.
29. Soderling, T. R. 1990. Protein kinases. J. Biol. Chem. 265:1823-1826.
30. Stokoe, D., S. G. MacDonald, K. Caddwallader, M. Symons, and J. F. Hancock. 1994. Activation of Raf as a result of recruitment to the plasma membrane. Science 264:1463-1467.
31. Tu, H. Unpublished findings.
32. Tu, H., M. Barr, D. L. Dong, and M. Wigler. 1997. Multiple regulatory domains on the Byr2 protein kinase. Mol. Cell. Biol. 17:5876-5887.
33. Van Aelst, L., M. Barr, S. Marcus, A. Polverino, and M. Wigler. 1993. Complex formation between RAS and RAF and other protein kinases. Proc. Natl. Acad. Sci. USA 90:6213-6217.
34. Vojtek, A., S. M. Hollenberg, and J. A. Cooper. 1993. Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 74:205-214.
35. Ward, Y., S. Gupta, P. Jensen, M. Wartmann, R. J. Davis, and K. Kelly. 1994. Control of MAP kinase activation by the mitogen-induced threonine/ tyrosine phosphatase PAC1. Nature 367:651-654.
36. White, M., C. Nicolette, A. Minden, A. Polverino, L. Van Aelst, M. Karin, and M. Wigler. 1995. Multiple RAS functions can contribute to mammalian cell transformation. Cell 80:533-541.
37. Wu, C., M. Whiteway, D. Y. Thomas, and E. Leberer. 1995. Molecular characterization of Ste20p, a potential mitogen-activated protein or extra-cellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J. Biol. Chem. 270:15984-15992.
38. Yang, P., S. Kansra, R. A. Pimental, M. Gibreth, and S. Marcus. 1998. Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast. J. Biol. Chem. 273:18481-18489.
39. Zhang, S., J. Han, M. Sells, J. Chernoff, U. G. Knaus, R. J. Ulevitch, and G. M. Bokoch. 1995. Rho family GTPases regulate p38 mitogen-activated protein kinase through the downstream mediator Pak1. J. Biol. Chem. 270: 23934-23936.
40. Zhao, Z. S., E. Manser, X. Q. Chen, C. Chong, T. Leung, and L. Lim. 1998. A conserved negative regulatory region in αPAK: inhibition of PAK kinases reveals their morphological roles downstream of Cdc42 and Rac1. Mol. Cell. Biol. 18:2153-2163.
41. Zhou, Y., X. Zhang, and R. H. Ebright. 1991. Random mutagenesis of gene-sized DNA molecules by use of PCR with TAQ DNA polymerase. Nucleic Acids Res. 19:6052.