5-Azacytidine prevents transgene methylation in vivo

Gene Therapy

Volumn 6, Issue 4, 1999, Pages 703-707

  Di Ianni, M ^a   Terenzi, A ^a   Perruccio, K ^a   Ciurnelli, R ^a   Lucheroni, F ^a   Benedetti, R ^a   Martelli, M F ^a   Tabilio, A ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

5 Azacytidine; DNA methylation; Retroviral vectors; SCID mice

Indexed keywords

AZACITIDINE; BETA GALACTOSIDASE; VIRUS VECTOR;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DEMETHYLATION; DNA METHYLATION; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RETROVIRUS; SUICIDE GENE; TRANSGENE; TUMOR VOLUME;

ANIMALS; ANTIVIRAL AGENTS; AZACITIDINE; DNA METHYLATION; GANCICLOVIR; GENE EXPRESSION; GENE THERAPY; GENETIC VECTORS; HUMANS; LAC OPERON; MICE; MICE, SCID; RETROVIRIDAE; THYMIDINE KINASE; U937 CELLS;

ANIMALIA; BACTERIA (MICROORGANISMS); UNIDENTIFIED RETROVIRUS;

EID: 0032907381     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300848     Document Type: Article

References (19)

1. Miller AD. Human gene therapy comes of age. Nature 1992; 357: 455-460.
2. Brenner MK. Gene transfer to hematopoietic cells. Molec Med 1996; 335: 337-339.
3. Cedar H. DNA methylation and gene activity. Cell 1988; 53: 3-4.
4. Challita PM, Kohn DB. Lack of expression from a retroviral vector after transduction of murine hematopoietic stem cells is associated with methylation in vivo. Proc Natl Acad Sci USA 1994; 91: 2567-2571.
5. Hoeben RC et al. Inactivation of the Moloney murine leukemia virus long terminal repeat in murine fibroblast cell line is associated with methylation and dependent on its chromosomal position. J Virol 1991; 65: 904-912.
6. Jaenish R, Schienke A, Harbers K. Treatment of mice with 5-azacytidine efficiently activates silent retroviral genomes in different tissues. Proc Natl Acad Sci USA 1985; 82: 1451-1455.
7. Ghattas IR, Sanes JR, Majors JE. The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and embryos. Mol Cell Biol 1991; 11: 5848-5859.
8. Di Ianni M et al. Retroviral transfer of herpes simplex virus-thymidine kinase and beta-galactosidase genes into U937 cells with bicistronic vector. Leukemia Res 1997; 21: 951-959.
9. Nolan GP, Fiering S, Nicolas JF, Herzenberg LA. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on β-D-galactosidase activity after transduction of E. coli lacZ. Proc Natl Acad Sci USA 1988; 85: 2603-2607.
10. Janher D, Haase K, Mulligan R, Jaenisch R. Insertion of the bacterial gpt gene into the germ line of mice by retroviral infection. Proc Natl Acad Sci USA 1985; 82: 6927-6931.
11. Stewart CL, Schuetze S, Vanek M, Wagner EF. Expression of retroviral vectors in transgenic mice obtained by embryo infection. EMBO J 1987; 6: 383-388.
12. Gautsch JW, Wilson MC. Delayed de nova methylation in teratocarcinoma suggests additional tissue-specific mechanisms for controlling gene expression. Nature 1983; 301: 32-37.
13. Stuhlmann H, Jahner D, Jaenisch R. Infectivity and methylation of retroviral genomes is correlated with expression in the animal. Cell 1981; 26: 221-232.
14. Chen WY et al. Reactivation of silenced, virally transduced genes by inhibitors of histone deacetylase. Proc Natl Acad Sci USA 1997; 94: 5798-5803.
15. Lowrey CH, Nienhuis AW. Brief report: treatment with azacytidine of patients with end-stage β-thalassemia. New Engl J Med 1993; 329: 845-848.
16. Markowitz DS, Goff S, Bank A. A safe packaging line for gene transfer: separating viral genes on two different plasmids. J Virol 1988; 62: 1120-1124.
17. Markowitz DS, Goff S, Bank A. Construction and use of a safe and efficient packaging cell line. Virology 1988; 85: 400-406.
18. Lynch CM, Miller AD. Production of high-titer helper virus-free retroviral vectors by cocultivation of packaging cells with different hostranges. J Virol 1991; 65: 3887-3890.
19. Macgregor GR, Mogg AE, Burke JF, Caskey CT. Histochemical staining of clonal mammalian cell lines expressing E. coli β-galactosidase indicates heterogeneous expression of the bacterial gene. Somat Cell Mol Genet 1987; 13: 253-265.