Analysis of CHO-K1 cell growth in a fixed bed bioreactor using magnetic resonance spectroscopy and imaging

Cytotechnology

Volumn 30, Issue 1-3, 1999, Pages 121-132

  Thelwall, Peter E ^a   Brindle, Kevin M ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Bioreactor; Cell volume; Imaging; Magnetic resonance

Indexed keywords

BIOREACTOR; CELL GROWTH; NUCLEAR MAGNETIC RESONANCE IMAGING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

EID: 0032901674     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008039011960     Document Type: Article

References (32)

1. Banik GG and Heath CA (1995) Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture. Biotechnol Bioeng 48: 289-300.
2. 31P nuclear magnetic resonance spectroscope probe of intracellular volume in mammalian cell cultures. Biochemistry 32: 4665-4670.
3. Borys MC, Linzer DIH and Papoutsakis ET (1993) Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese Hamster Ovary (CHO) cells. Bio/Technology 11: 720-724.
4. Blute T, Gillies RJ and Dale BE (1988) Cell density measurements in hollow fibre bioreactors. Biotechnol Prog 4: 202-209.
5. Brindle KM (1998) Investi gating the performance of intensive mammalian cell bioreactor systems using magnetic resonance imaging and spectroscopy. Biotech Genetic Eng Rev 15: 499-520.
6. Callies R, Jackson ME and Brindle KM (1994) Measurements of the growth and distribution of mammalian cells in a hollow-fiber bioreactor using nuclear magnetic resonance imaging. Bio/Technology 12: 75-78.
7. Chresand TJ, Gillies RJ and Dale BE (1988) Optimum fiber spacing in a hollow fiber bioreactor. Biotechnol Bioeng 32: 983-992.
8. Donoghue C, Brideau M, Newcomer P, Pangrle B, DiBiasio D, Walsh E and Moore S (1992) Use of magnetic resonance imaging to analyse the performance of hollow fibre bioreactors. Ann NY Acad Sci 665: 285-300.
9. Drury DD, Dale BE and Gillies RJ (1988) Oxygen transfer properties of a bioreactor for use within a nuclear magnetic resonance spectrometer. Biotechnol Bioeng 32: 966-974.
10. Fernandes EJ (1996) Nuclear magnetic resonance spectroscopy and imaging. In: Willaert RG, Baron GV and de Backer L (eds) Immobilised living cell systems. (pp. 117-146) John Wiley & Sons, Chichester, UK.
11. Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strange PG, Tong JM and Bull AT (1992) Glucose-limited chemostat culture of Chinese Hamster Ovary cells producing recombinant human interferon-γ. Biotechnol Bioeng 39: 327-335.
12. Gillies RJ, MacKenzie NE and Dale BE (1989) Analyses of bioreactor performance by nudear magnetic resonance spectroscopy. Bio/Technology 7: 50-54.
13. 31P NMR. Magn Reson Med 18: 181-192.
14. Hammer BE, Heath CA, Mirer SD and Belfort G (1990) Quantitative flow measurements in bioreactors by nuclear magnetic resonance imaging. Bio/Technology 8: 327-330.
15. Hubbell JA and Langer R (1995) Tissue engineering. Chem Eng News 73: 42-54.
16. Jauregui HO, Chowdhury NR and Chowdhury JR (1996) Use of mammalian liver cells for artificial liver support. Cell Transplantation 5: 353-367.
17. Knazek RA, Gullino PM, Kholer PO and Dedrick RL (1972) Cell growth on artificial capillaries: an approach to tissue growth in vitro. Science 178: 65-67.
18. Knight P (1989) Hollow fiber bioreactors for mammalian cell culture. Bio/Technology 7: 459-461.
19. Langer R and Vacanti JP (1993) Tissue engineering. Science 260: 920-926.
20. Lin AA, Kimura R and Miller WM (1993) Production of tPA in recombinant CHO cells under oxygen-limited conditions. Biotechnol Bioeng 42: 339-350.
21. Ljunggren J and Häggström L (1994) Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures. Biotechnol Bioeng 44: 808-818.
22. Lund P (1985) In: Bergmeyer HU (ed) Methods of Enzymatic Analysis. Vol. 8. (pp. 357-363) Academic Press, New York.
23. Mancuso A, Fernandes EJ, Blanch HW and Clarke DS (1990) A nuclear magnetic resonance technique for determining hybridoma cell concentration in hollow fibre bioreactors. Bio/Technology 8: 1282-1285.
24. Neermann J and Wagner R (1996) Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and pri mary liver cells. J Cell Phys 166: 152-169.
25. Noll T, Biselli M and Wandrey C (1996) On-line biomass monitoring of immobilised hybridoma cells by dielectrical measurements. In: Carrondo MJT, Griffiths B and Moreira JLP (eds) Animal Cell Technology, (pp. 289-294), Kluwer Academic Publishers, The Netherlands
26. Ong CP, Pörtner R, Märkl H, Yamazsaki Y, Yasuda K and Matsumura M (1994) High density cultivation of hybridoma in charged porous carriers. J Biotechnol 34: 259-268.
27. Piret JM and Cooney CL (1990a) Mammalian cell and protein distributions in ultrafiltration bioreactors. Biotechnol Bioeng 36: 902-910.
28. Piret JM and Cooney CL (1990b) Model of oxygen transport limitations in hollow fibre bioreactors. Biotechnol Bioeng 37: 80-92.
29. Seewoster T and Lehmann J (1997) Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells. Biotechnol Bioeng 55: 793-797.
30. van Zijl PCM, Moonen CTW, Faustino P, Pekar J, Kaplan O and Cohen JS (1991) Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy. Proc Natl Acad Sci USA 88: 3228-3232.
31. Wiesmann R, Maier ST, Marx U and Buchholz R (1994) Characterisation of oxygen transfer in a membrane-aerated hollow-fibre bioreactor using modified microcoaxial needle electrodes. Appl Microbiol Biotechnol 41: 531-536.
32. Williams SNO, Callies RM and Brindle KM (1997) Mapping of oxygen tension and cell distribution in a hollow-fiber bioreactor using magnetic resonance imaging. Biotechnol Bioeng 58: 56-61.