A computer-assisted two-dimensional gel electrophoresis approach for studying the variations in protein expression related to an induced functional repression of NFκB in lymphoblastoid cell lines

Electrophoresis

Volumn 20, Issue 4-5, 1999, Pages 1017-1026

  Joubert Caron, Raymond ^a   Feuillard, Jean ^a   Kohanna, Sylvie ^a   Poirier, Florence ^a   Le Caër, Jean Pierre ^b   Schuhmacher, Marino ^c   Bornkamm, Georg W ^c   Polack, Axel ^c   Caron, Michel ^a   Bladier, Dominique ^a   Raphaël, Martine ^a  

^a UNIVERSITÉ PARIS 13   (France)

^b Laboratoire de Neurobiologie   (France)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Lymphoblastoid cells; Transcription factors NF B, I B; Two dimensional gel electrophoresis

Indexed keywords

CELL CULTURE; ELECTROPHORESIS; PHYSIOLOGICAL MODELS; TRANSCRIPTION;

ANALYTICAL METHODOLOGY; CELL LINES; COMPUTER ASSISTED; DISCRETE SETS; LYMPHOBLASTOID CELLS; OVEREXPRESSIONS; PROTEIN EXPRESSIONS; TRANSCRIPTION FACTOR NFΚB, IΚB; TWO-DIMENSIONAL GEL ELECTROPHORESIS; VISUAL VALIDATION;

TRANSCRIPTION FACTORS;

IMMUNOGLOBULIN ENHANCER BINDING PROTEIN;

COMPUTER ANALYSIS; COMPUTER PROGRAM; CONFERENCE PAPER; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN EXPRESSION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0032901256     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(19990101)20:4/5<1017::AID-ELPS1017>3.0.CO;2-K     Document Type: Conference Paper

References (35)

1. Huen, D. S., Henderson, S. A., Croom-Carter, D. Rowe, M., Oncogene 1995, 10, 549-560.
2. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
3. Görg, A., Nature 1991, 349, 545-546.
4. Görg, A., Fawcett, J. S., Chrambach, A., Adv. Electrophoresis 1988, 2, 1-43.
5. Görg, A., Postel, W., Günther, S., Electrophoresis 1994, 15, 1205-1211.
6. Righetti, P. G., Immobilized pH gradients. Theory and Methodology, Elsevier, Amsterdam 1990.
7. Appel, R. D., Palagi, P. M., Walther, D., Vargas, J. R., Sanchez, J.-C., Ravier, F., Pasquali, C., Hochstrasser, D. F., Electrophoresis 1997, 18, 2724-2734.
8. Appel, R. D., Vargas, J. R., Palagi, P. M., Walther, D., Hochstrasser, D. F., Electrophoresis 1997, 18, 2735-2748.
9. Klose, J., Feller, M., Electrophoresis 1981, 2, 12-24.
10. Sanchez, J.-C., Appel, R. D., Golaz, O., Pasquali, C., Ravier, F., Bairoch, A., Hochstrasser, D. F., Electrophoresis 1995, 16, 1131-1151.
11. Celis, J. E., Rasmussen, H. H., Olsen, E., Madsen, P., Leffers, H., Honoré, B., Dejgaard, K., Gromov, P., Vorum, H., Vassilev, A., Baskin, Y., Lin, X., Celis, A., Basse, B., Lauridsen, J. B., Ratz, G. P., Andersen, A. H., Walbum, E., Kjaergaard, I., Andersen, I., Puype, M., Van Damme, J., Vanderkerckhove, J., Electrophoresis 1994, 15, 1349-1458.
12. Lutomski, D., Caron, M., Cornillot, J.-D., Bourin, P., Dupuy, C., Pontet, M., Bladier, D., Joubert-Caron, R., Electrophoresis 1996, 17, 600-606.
13. URL: http://expasy.hcuge.ch/ch2d/
14. URL: http://www.anl.gov/CMB/PMG/
15. URL: http://WWW.LSBC.COM/2dmaps/patterns.htm
16. URL: http://biobase.dk/cgi-bin/celis
17. URL: http://www.harefield.nthames.nhs.uk/nhli/protein/
18. URL: http://www.mdc-berlin.de/~emu/heart/
19. URL: http://www-lecb.ncifcrf.gov/PDD/
20. URL: http://iupucbio1.iupui.edu/franw/molan.htm
21. URL: http://www.ludwig.edu.au/www/jpsl/jpslhome.html
22. URL http://www.tmig.or.jg/2D/2D_Home.html
23. URL: http://www-smbh.univ-paris13.fr/biochemistry/biochimie.htm
24. Polack, A., Hortnagel, K., Pajic, A., Christoph, B., Baier, B., Falk, M., Mautner, J., Geltinger, C., Bornkamm, G. W., Kempkes, B., Proc. Natl. Acad. Sci. USA 1996, 93, 10411-10416.
25. Mucke, S., Polack, A., Pawlita, M., Zehnpfennig, D., Massoudi, N., Bohlen, H., Doerfler, W., Bornkamm, G., Diehl, V., Wolf, J., Gene Ther. 1997, 4, 82-92.
26. Bradford, M. M., Anal. Biochem. 1976, 72, 335-340.
27. Heukeshoven, J., Dernick, R., Electrophoresis 1985, 6, 103-112.
28. Hoogland, C., Sanchez, J.-C., Tonella, L., Bairoch, A., Hochstrasser, D. F., Appel, R. D., Nucleic Acids Res. 1998, 26, 332-333.
29. Lutomski, D., Fouillit, M., Bourin, P., Mellottee, D., Denize, N., Pontet, M., Bladier, D., Caron, M., Joubert-Caron, R., Glycobiology 1997, 7, 1193-1199.
30. Pun, T., Hochstrasser, D. F., Appel, R. D., Funk, M., Villars-Augsburger, V., Pellegrini, C., Appl. Theor. Electrophor. 1988, 1, 3-9.
31. Armitage, P., Berry, G., Statistical Methods and Biomedical Research, Blackwell Scientific Publications, Oxford 1987.
32. Unli, M., Morgan, M. E., Minden, J. S., Electrophoresis 1997, 18, 2071-2077.
33. Merril, C. L., Creed, G. J., Joy, J., Olson, A. D., Appl. Theor. Electrophor. 1993, 3, 329-333.
34. Gossen, M., Bujard, H., Proc. Natl. Acad. Sci. USA 1992, 89, 5547-5551.
35. Furth, P., St. Onge, L., Böger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H., Hennighausen, L., Proc. Natl. Acad. Sci. USA 1994, 91, 9302-9306.