Pemphigus vulgaris-IgG causes a rapid depletion of desmoglein 3 (Dsg3) from the Triton X-100 soluble pools, leading to the formation of Dsg3- depleted desmosomes in a human squamous carcinoma cell line, DJM-1 cells

Journal of Investigative Dermatology

Volumn 112, Issue 1, 1999, Pages 67-71

  Aoyama, Yurni ^a   Kitajima, Yasuo ^a  

^a GIFU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Autoimmune blistering disease; Desmoplakin; Heratin intermediate filament; Plakoglobin

Indexed keywords

CYTOKERATIN; DESMOGLEIN; DESMOPLAKIN; IMMUNOGLOBULIN G; KERATIN; PLAKOGLOBIN; TRITON X 100;

ARTICLE; CANCER CELL CULTURE; CELL MEMBRANE; CELL SURFACE; CONTROLLED STUDY; CYTOSKELETON; CYTOSOL; DESMOSOME; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE MICROSCOPY; PEMPHIGUS VULGARIS; PRIORITY JOURNAL; SQUAMOUS CELL CARCINOMA;

EID: 0032900965     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1747.1999.00463.x     Document Type: Article

References (25)

1. Allen E, Yu QC, Fuchs E: Mice expressing a mutant desmosomal cadherin exhibit abnormalities in desmosomes, proliferation, and epidermal differentiation. J Cell Biol 133:1367-1382, 1996
2. Amagai M: Adhesion molecules. Keratinocyte-keratinocytes interaction; cadherins and pemphigus. J Invest Dermatol 104:146-152, 1995
3. Amagai M, Koch PJ, Nishikawa T, Stanley JR: Pemphigus vulgaris antigen (Desmoglein 3) is localized in the lower epidermis, the site of blister formation in patients. J Invest Dermatol 106:351-355, 1996
4. Andrew P, Kowalezyk AP, Anderson JE, Bougwardt JE, Hashimoto T, Stanley JR, Green KJ: Pemphigus sera recognize conformationally sensitive epitopes in the aminoterminal regions of desmogelin-1. J Invest Dermatol 105:147-152, 1995
5. Arnemann Sullivan KH, Magee AI, King IA, Buxton RS: Stratification related expression of isoforms of the desmosomal cadherins in human epidermis. J Cell Sci 104:741-750, 1993
6. Buxton RS, Cowin P, Franke WW, et al: Nomenclature of desmosomal cadherins. J Cell Biol 121:481-483, 1993
7. Esaki C, Seishima M, Yamada T, Osada K, Kitajima Y: Pharmacologic evidence for involvement of phospholipase C in pemphigus IgG-induced inositol 1,4,5-trisphosphate generation, intracellular calcium increase, and plasminogen activator secretion in DJM-1 cells, a squamous cell carcinoma line. J Invest Dermatol 105:329-333, 1995
8. Eyre RW, Stanley JR: Identification of pemphigus vulgaris antigen extracted from normal human epidermis and comparison with pemphigus foliaceus antigen. J Clin Invest 81:807-812, 1988
9. Hashimoto K, Shafran KM, Webber PA, Lazarus GS, Singer KH: Anti-cell surface pemphigus antibody stimulates plasminogen activator activity of human epidermal cells. A mechanism for the loss of epidermal cohesion and blister formation. J Exp Med 157:259-272, 1983
10. Hashimoto T, Ogawa MM, Konohana A, Nishikawa T: Detection of pemphigus vulgaris and pemphigus foliaceus antigens by immunoblot analysis using different antigen sources. J Invest Dermatol 94:327-331, 1990
11. Karpati S, Amagai M, Prussick R, Cehrs K, Stanley JR: Pemphigus vulgaris antigen, a desmoglein type of cadherin, is located within keratinocyte desmosomes. J Cell Biol 122:409-415, 1993
12. Kitajima Y: Adhesion molecules in the pathophysiology of bullous deseases. Eur J Dermatol 6:399-405, 1996
13. ++ concentration. J Invest Dermatol 89:167-171, 1987
14. Kitajima Y, Inoue S, Nagao S, Nagata K, Yaoita H, Nozawa Y: Biphasic effects of 12-0-tetradecanoylphorbol-13-acetate on the cell morphology of low calcium-grown human epidermal carcinoma cells: involvement of translocation and down regulation of protein kinase C. Cancer Res 48:964-970, 1988
15. Koch PJ, Goldschmidt MD, Walsh MJ, Zimbelmann R, Troyanoysky R, Franke WW: Complexity and expression patterns of the desmosomal cadherins. Proc Nad Acad Sci USA 89:353-357, 1992.
16. Koch PJ, Mahoney MG, Ishikawa H, et al: Targeted disruption of the pemphigus vulgaris antigen (desmoglein 3) gene in mice causes loss of keratinocyte cell adhesion with a phenotype similar to pemphigus vulgaris. J Cell Biol 137:1091-1102, 1997
17. Korman N, Eyre RW, Klau-kovtun V, Stanley JR: Demonstration of an adhering junction molecule (plakoglobin) in the autoantigens of pemphigus foliaceus and pemphigus vulgaris. N Engl Med 321:631-635, 1985
18. Koulu L, Kusurni A, Steinberg MS, Klaus-Kovturn V, Stanley JR: Human autoantibodies against a desmosomal core protein in pemphigus foliaceus. J Exp Med 160:1509-1518, 1984
19. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685, 1970
20. Morioka S, Naito K, Ogawa H: The pathogenic role of pemphigus antibodies and proteinase in epidermal acantholysis. J Invest Dermatol 76:337-341, 1981
21. Osada K, Seishirna M, Kitajima Y: Pemphigus IgG activates and translocates protein kinase C from the cytosol to the particulate/cytoskeleton fractions in human keratinocytes. J Invest Dermatol 108:482-487, 1997
22. Schaefer S, Stump S, Franke WW: Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation 60:99-108, 1996
23. Seishima M, Osada K, Mori S, Hashimoto T, Kitajima Y: Pemphigus IgG, but not bullous pemphigoid IgG, causes a transient increase in intracellular calcium and inositol 1,4,5-trisphosphate in DJM-1 cells, a squamous cell carcinoma line. J Invest Dermatol 104:33-37, 1995
24. Seishima M, Satoh S, Nojifi M, Osada K, Kitajima Y: Pemphigus IgG induces expression of urokinase plasminogen activator receptor on the cell surface of cultured keratinocytes. J Invest Dermatol 109:650-655, 1997
25. Shimizu H, Masunaga T, Ishilco A, Kikuchi A, Hashimoto T, Nishikawa T: Pemphigus vulgaris and pemphigus foliaceus sera show an inversely graded binding pattern to Extracellular regions of desmosomes in different layers of human epidermis. J Invest Dermatol 105:153-159, 1995