Identification of RNAs that bind to a specific protein using the yeast three-hybrid system

RNA

Volumn 5, Issue 4, 1999, Pages 596-601

  Sengupta, Dhruba J ^a   Wickens, Marvin ^b   Fields, Stanley ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Hybrid protein; Reporter gene assay; Snp1 protein; Transcriptional activator; U1 RNA

Indexed keywords

COAT PROTEIN; HYBRID PROTEIN; RNA BINDING PROTEIN;

ARTICLE; BACTERIOPHAGE; BINDING SITE; HYBRID; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN INTERACTION; PROTEIN RNA BINDING; RNA ANALYSIS; RNA BINDING; RNA SEQUENCE; TRANSCRIPTION INITIATION; YEAST;

CAPSID; CAPSID PROTEINS; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GENE LIBRARY; GENES, REPORTER; LIGANDS; PROMOTER REGIONS (GENETICS); RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

UNIDENTIFIED BACTERIOPHAGE;

EID: 0032900053     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838299002113     Document Type: Article

References (15)

1. Bacharach E, Goff SP. 1998. Binding of the human immunodeficiency virus type 1 Gag protein to the viral RNA encapsidation signal in the yeast three-hybrid system. J Virol 72:6944-6949.
2. Gietz D, St Jean A, Woods RA, Schiestl RH. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20:1425.
3. Gold L, Polisky B, Uhlenbeck O, Yarus M. 1995. Diversity of oligonucleotide functions. Annu Rev Biochem 64:763-797.
4. Gorodkin J, Heyer LJ, Stormo GD. 1997. Finding the most significant common sequence and structure motifs in a set of RNA sequences. Nucleic Acids Res 25:3724-3732.
5. Iwabuchi K, Li B, Bartel P, Fields S. 1993. Use of the two-hybrid system to identify the domain of p53 involved in oligomerization. Oncogene 8:1693-1696.
6. Kao HY, Siliciano PG. 1992. The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene. Nucleic Acids Res 20:4009-4013.
7. Liao X, Kretzner L, Seraphin B, Rosbash M. 1990. Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function. Genes & Dev 4:1766-1774.
8. Martin F, Schaller A, Eglite S, Schumperli D, Muller B. 1997. The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein. EMBO J 16:769-778.
9. Matzura O, Wennborg A. 1996. RNAdraw: An integrated program for RNA secondary structure calculation and analysis under 32-bit Microsoft Windows. Comput Appl Biosci 12:247-249.
10. Miller JH. 1972. Experiments in molecular genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
11. SenGupta DJ, Zhang B, Kraemer B, Pochart P, Fields S, Wickens M. 1996. A three-hybrid system to detect RNA - protein interactions in vivo. Proc Natl Acad Sci USA 93:8496-8501.
12. Surowy CS, van Santen VL, Scheib-Wixted SM, Spritz RA. 1989. Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA. Mol Cell Biol 9:4179-4186.
13. Tuerk C, Gold L. 1990. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249:505-510.
14. Wang ZF, Whitfield ML, Ingledue TC 3rd, Dominski Z, Marzluff WF. 1996. The protein that binds the 3′ end of histone mRNA: A novel RNA-binding protein required for histone pre-mRNA processing. Gene & Dev 10:3028-3040.
15. Zhang B, Gallegos M, Puoti A, Durkin E, Fields S, Kimble J, Wickens MP. 1997. A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line. Nature 390:477-484.