Proteolytic cleavage of the A subunit is essential for maximal cytotoxicity of Escherichia coli O157:H7 Shiga-like toxin-1

Microbiology

Volumn 145, Issue 5, 1999, Pages 999-1004

  Lea, Nicholas ^a,b   Lord, J Michael ^a   Roberts, Lynne M ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b KING S COLLEGE HOSPITAL   (United Kingdom)

Author keywords

Proteolytic processing; Shiga like toxin 1; Toxin A subunit; Toxin activation

Indexed keywords

FURIN; PROTEIN SUBUNIT; SHIGA TOXIN;

ANIMAL CELL; ARTICLE; CYTOTOXICITY; ENDOCYTOSIS; ESCHERICHIA COLI; MAMMAL CELL; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; SITE DIRECTED MUTAGENESIS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; NEGIBACTERIA;

EID: 0032898861     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-5-999     Document Type: Article

References (25)

1. Brown, J. E., Ussery, M. A., Leppla, S. H. & Rothman, S. W. (1980). Inhibition of protein synthesis by Shiga toxin: activation of the toxin and inhibition of peptide elongation. FEBS Lett 117, 84-88.
2. Burgess, B. J. & Roberts, L. M. (1993). Proteolytic cleavage at arginine residues within the hydrophilic disulphide loop of the Escherichia coli Shiga-like toxin I A subunit is not essential for cytotoxicity. Mol Microbiol 10, 171-179.
3. Calderwood, S. B., Auclair, F., Donohue-Rolfe, A., Keusch, G. T. & Mekalanos, J. J. (1987). Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proc Natl Acad Sci USA 84, 4364-4368.
4. Chiron, M. E., Fryling, C. M. & FitzGerald, D. J. (1994). Cleavage of pseudomonas exotoxin and diphtheria toxin by a furin-like enzyme prepared from beef liver. J Biol Chem 269, 18167-18176.
5. Drazin, R., Kandel, J. & Collier, R. J. (1971). Structure and activity of diphtheria toxin. II. Attack by trypsin at a specific site within the intact toxin molecule. J Biol Chem 246, 1504-1510.
6. Endo, Y., Tsurugi, K., Yutsudo, T., Takeda, Y., Ogasawara, T. & Igarashi, K. (1988). Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins. Eur J Biochem 171, 45-50.
7. Fraser, M. E., Chernaia, M. M., Kozlov, Y. V. & James, M. N. G. (1994). Crystal structure of the holotoxin from Shigella dysenteriae at 2·5 Å resolution. Nat Struct Biol 1, 59-64.
8. Garred, O., van Deurs, B. & Sandvig, K. (1995a). Furin-induced cleavage and activation of Shiga toxin. J Biol Chem 270, 10817-10821.
9. Garred, O., Dubinina, E., Holm, P. K., Olsnes, S., van Deurs, B., Kozlov, J. V. & Sandvig, K. (1995b). Role of processing and intracellular transport for optimal toxicity of Shiga toxin and toxin mutants. Exp Cell Res 218, 39-49.
10. Gordon, V. M. & Leppla, S. H. (1994). Proteolytic activation of bacterial toxins: role of bacterial and host cell proteases. Infect Immun 62, 333-340.
11. Kongmuang, U., Honda, T. & Miwatani, T. (1988). Effect of nicking on Shiga-like toxin-I of enterohaemorrhagic Escherichia coli. FEMS Microbiol Lett 56, 105-108.
12. Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.
13. Lindberg, A. A., Brown, J. E., Stromberg, N., Westling-Ryd, M., Schultz, J. E. & Karlsson, K.-A. (1987). Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type 1. J Biol Chem 262, 1779-1785.
14. May, M. J., Hartley, M. R., Roberts, L. M., Krieg, P. A., Osborn, R. W. & Lord, J. M. (1989). Ribosome inactivation by ricin A chain: a sensitive method to assess the activity of wild-type and mutant polypeptides. EMBO J 8, 301-308.
15. Moehring, J. M., Inocencio, N. M., Robertson, B. J. & Moehring, T. J. (1993). Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion. J Biol Chem 268, 2590-2594.
16. Molloy, S. S., Bresnahan, P. A., Leppla, S. H., Klimpel, K. R. & Thomas, G. (1992). Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen. J Biol Chem 267, 16396-16402.
17. Muller, D., Vic, G., Critchley, P., Crout, D. H. G., Lea, N., Roberts, L. M. & Lord, J. M. (1998). Chemical synthesis of globotriose and galabiose: relative stabilities of their complexes with Escherichia coli Shiga-like toxin-I as determined by denaturation-titration with guanidinium chloride. J Chem Soc Perkin Trans 1, 2287-2294.
18. Ogata, M., Chaudhary, V. K., Pastan, I. & FitzGerald, D. J. (1990). Processing of Pseudomonas exotoxin by a cellular protease results in the generation of a 37,000-Da toxin fragment that is translocated to the cytosol. J Biol Chem 265, 20678-20685.
19. Olsnes, S., Reisbig, R. & Eiklid, K. (1981). Subunit structure of Shigella toxin. J Biol Chem 256, 8732-8738.
20. Samuel, J. E. & Gordon, V. M. (1994). Evidence that proteolytic separation of Shiga-like toxin type IIv A subunit into A1 and A2 subunits is not required for toxin activity. J Biol Chem 269, 4853-4859.
21. Takao, T., Tanabe, T., Hong, Y.-M., Shimonishi, Y., Kurazono, H., Yutsudo, T., Sasakawa, C., Yoshikawa, M. & Takeda, Y. (1988). Identity of the molecular structure of Shiga-like toxin 1 (VT1) from Escherichia coli O157: H7 with that of Shiga toxin. Microb Pathog 5, 357-369.
22. Tesh, V. L. & O'Brien, A. D. (1991). The pathogenic mechanisms of Shiga toxin and the Shiga-like toxins. Mol Microbiol 5, 1817-1822.
23. Tsuneoka, M., Nakayama, K., Hatsuzawa, K., Komada, M., Kitamura, N. & Mekada, E. (1993). Evidence for involvement of furin in cleavage and activation of diphtheria toxin. J Biol Chem 268, 26461-26465.
24. Vasil, M. L., Kabat, D. & Iglewski, B. H. (1977). Structure-activity relationships of an exotoxin of Pseudomonas aeruginosa. Infect Immun 16, 353-361.
25. 194. J Biol Chem 265, 20673-20677.