Osteoblastic differentiation and mRNA analysis of STRO-1-positive human bone marrow stromal cells using primary in vitro culture and poly (A) PCR

Journal of Pathology

Volumn 187, Issue 3, 1999, Pages 374-381

  Byers, Richard J ^a   Brown, Joanne ^a   Brandwood, Craig ^a   Wood, Peter ^a   Staley, Winifred ^a   Hainey, Linda ^a   Freemont, Anthony J ^a   Hoyland, Judith A ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Human marrow stromal cell culture; Osteoblast differentiation; Poly (A) RT PCR; STRO 1

Indexed keywords

ANTIBODY; COLLAGEN TYPE 1; COMPLEMENTARY DNA; DEXAMETHASONE; GENE PRODUCT; MESSENGER RNA; OSTEOCALCIN; OSTEOPONTIN;

ARTICLE; BONE MARROW; CELL CULTURE; CELL DIFFERENTIATION; CELL SELECTION; CONTROLLED STUDY; HISTOCHEMISTRY; HUMAN; HUMAN CELL; OSTEOBLAST; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STROMA CELL;

ADULT; BONE MARROW CELLS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; DNA, COMPLEMENTARY; HUMANS; MICROSCOPY, PHASE-CONTRAST; OSTEOBLASTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; STEM CELLS; STROMAL CELLS;

EID: 0032895854     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9896(199902)187:3<374::AID-PATH257>3.0.CO;2-V     Document Type: Article

References (29)

1. Rehman MTA, Hoyland JA, Denton J, Frcemont AJ. Histomorphometric patterns in post-menopausal osteoporosis. J Clin Pathol 1995; 48: 229-235.
2. Meunier PJ, Aaron J, Edouard C, Vignon G. Osteoporosis and the replacement of cell populations of the marrow by adipose tissue. Clin Orthop Rel Res 1971; 80: 147-154.
3. Owen M. Lineage of osteogenic cells and their relationship to the stromal system. In: Peck WA, ed. Bone & Mineral Research. Vol 3. Amsterdam: Elsevier, 1985; 1-25.
4. Friedenstein AJ. Precursor cells of mechanocytes. Int Rev Cytol 1976; 47: 327-359.
5. Ashton B, Allen T, Howlett C, Eaglestom C, Hatton A, Owen M. Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin Orthop 1980; 151: 294-307.
6. Urist M, Delange RJ, Finerman GAM. Bone cell differentiation and growth factors. Science 1983; 220: 680-686.
7. Nijweide PJ, Burger EH, Feyen JHM. Cells of bone: proliferation, differentiation and hormonal regulation. Physiol Rev 1986; 66: 855-886.
8. Goshima J, Goldberg V, Caplan A. The origin of bone formed in composite grafts of porous calcium phosphate ceramic and marrow cells. Clin Orthop 1991; 269: 274-283.
9. Owen M. Marrow stromal stem cells. J Cell Sci 1988; 510: 63-76.
10. Beresford JN. Osteogenic stem cells and the stromal system of bone and marrow. Clin Orthop Rel Res 1989; 240: 270-280.
11. Simmons DJ. The in vivo role of bone marrow fibroblast-like stromal cells. Calcif Tissue Int 1996; 58: 129-132.
12. Liu F, Malaval L, Gupta A, Aubin JE. Simultaneous detection of multiple bone related mRNAs and protein expression during osteoblast differentiation: polymerase chain reaction and immunocytochemical studies at the single cell level. Dev Biol 1994; 166: 220-234.
13. Bodine PVN, Trailsmith M, Komm BS. Development and characterisation of a conditionally transformed adult human osteoblastic cell line. J Bone Miner Res 1996; 11: 806-819.
14. Cheng S-L, Yang JW, Rifas L, Shang S-F, Avioli LV. Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology 1994; 134: 277-286.
15. Brady G, Iscove NN. Construction of cDNA libraries from sigle cells. Methods Enzymol 1993; 225: 611-623.
16. Simmons PJ, Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 1991; 78: 55-62.
17. + fraction of adult human bone marrow contains the osteogenic precursors. Blood 1994; 84: 4164-4173.
18. Leboy PS, Beresford JN, Devlin C, Owen ME. Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures. J Cell Physiol 1991; 146: 370-378.
19. Harrison A, Nuttal M, Holt I. Ashton B. Evidence of multipotency in cloned human marrow stromal cells. Bone and Tooth Society, March 1994.
20. Wergedal JE, Baylink DJ. Characterisation of cells isolated and cultured from human bone (41843). Proc Soc Exp Biol Med 1984; 176: 60-69.
21. Fogh J, Fogh JM, Orfeo T. One hundred and twenty-seven human tumor cell lines produced tumors in nude mice. J Natl Cancer Inst 1977; 59: 221-226.
22. Burstone MS. Hydrolytic enzymes in dentinogenesis and osteogenesis. In: Sognnaes RF, ed. Calcification in Biological Systems. Washington, DC: American Association of Advanced Science, 1960; 217-219.
23. Gilmore S, Whitmore SW, Bowers DE. A simple method using alizarin red for detection of calcium in epoxy resin embedded tissue. Stain Technol 1985; 61: 89-92.
24. Lowry OH, Roberts NR, Wu M, Hixen WS, Crawford D. The quantitative histochemistry of brain II. Enzyme measurements. J Biol Chem 1954; 207: 13-19.
25. Toshikatsu Matshymama KH, Lau W, Wergedal JE. Monolayer cultures of normal human bone cells contain multiple subpopulations of alkaline phosphatase positive cells. Calcif Tissue Int 1990; 47: 276-283.
26. Aubin JE, Liu F, Malaval L, Gupta AK. Osteoblast and chondroblast differentiation. Bone 1995; 17: 77S-83S.
27. Cumano A, Paige CJ, Iscove NN, Brady G. Bipotential precursors of B cells and macrophages in murine fetal liver. Nature 1992; 356: 612-615.
28. Joyner CJ, Bennett A, Triffitt JT. Identification and enrichment of human osteoprogenitor cells by using differentiation stage-specific monoclonal antibodies. Bone 1997; 21: 1-6.
29. Bruder SP, Horowitz MC, Mosca JD, Haynesworth SE. Monoclonal antibodies reactive with human osteogenic cell surface antigens. Bone 1997; 21: 225-235.