Comparison of two PF4/heparin ELISA assays for the laboratory diagnosis of heparin-lnduced thrombocytopenia

Seminars in Thrombosis and Hemostasis

Volumn 25, Issue 2 SUPPL. 1, 1999, Pages 51-56

  Izban, Keith F ^a   Lietz, Helen W ^a   Hoppensteadt, Debra A ^a   Jeske, Walter P ^a   Fareed, Jawed ^a   Bakhos, Mamdouh ^b   Walenga, Jeanine M ^a  

^a LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

^b NONE

Author keywords

Anti PF4 heparin antibodies; Assays; ELISA; Heparin induced thrombocytopenia; Laboratory diagnosis; Serotonin release assay

Indexed keywords

CERTOPARIN; DRUG ANTIBODY; HEPARIN; HEPARIN ANTIBODY; SEROTONIN; THROMBOCYTE FACTOR 4; UNCLASSIFIED DRUG;

ANTIBODY DETECTION; ANTICOAGULATION; CONFERENCE PAPER; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; HEPARINIZATION; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; RELIABILITY; SEROTONIN RELEASE; THROMBOCYTOPENIA; THROMBOSIS;

ADULT; ANTICOAGULANTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEPARIN; HUMANS; MIDDLE AGED; PLATELET FACTOR 4; SEROTONIN; THROMBOCYTOPENIA;

EID: 0032895632     PISSN: 00946176     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (23)

1. Powers PJ, Kelton JG, Carter CJ. Studies on the frequency of heparin-associated thrombocytopenia. Thromb Res 1984;33: 439-443
2. King DJ, Kelton JG. Heparin-associated thrombocytopenia. Ann Intern Med 1984;100:535-540
3. Wallis DE, Lewis BH, Pifarré R, Scanlon PJ. Active surveillance for heparin-induced thrombocytopenia and thromboembolism. Chest 1904;106:120S(Abst)
4. Walls JT, Curtis JJ, Silver D, Boley TM. Heparin-induced thrombocytopenia in patients who undergo open heart surgery. Surgery 1990;108:686-693
5. Amiral J, Bridey F, Dreyfus M, et al. Platelet factor 4 complexed to heparin is the target for antibodies generated in heparin-induced thrombocytopenia. Thromb Haemostas 1992;68:95-96
6. Chong BH, Ismail F, Chesteman CN, Berndt MD. Heparin-induced thrombocytopenia: Mechanism of interaction of the heparin-dependent antibody with platelets. Br J Haematol 1989; 73:235-240
7. Greinacher A, Pötzsch B, Amiral J, et al. Heparin-associated thrombocytopenia: Isolation of the antibody and characterization of a multimolecular PF4-heparin complex as the major antigen. Thromb Hemostas 1994;71:247-251
8. Ziporen L, Park KS, Li ZQ, et al. The C-terminus of platelet factor 4 is required for recognition by heparin-induced thrombocytopenia antibodies. Blood 1997;90:403(Abst)
9. Visentin GP, Moghaddam M, Collins JL, McFarland JG, Aster RH. Antibodies associated with heparin-induced thrombocytopenia report conformational changes in platelet factor four induced by linear, polyanionic compounds. Blood 1997;90: 460(Abst)
10. Chong BH, Newman PM. The antigenic epitope in heparin-induced thromhocytopenia resides solely on platelet factor 4, Blood 1997;90:461(Abst)
11. Stewart MW, Etches WS, Boshkov LK, Gordon PA. Heparin-induced thrombocytopenia: An improved method of detection based on lumi-aggregometry. Br J Haematol 1995;91:173-177
12. Sheridan D, Carter C, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia. Blood 1986;67:27-30
13. Lee DH, Warkentin TE, Denomme GA, Hayward CPM, Kelton JG. A diagnostic test for heparin-induced thromhocytopenia: Detection of platelet microparticles using flow cytometry. Br J Haematol 1996;95:724-731
14. Tomer A. Rapid, sensitive and specific functional flow cytometric assay for the diagnosis of heparin-induced thrombocytopenia (HIT). Blood 1996;88;317(Abst)
15. Jeske W, Szatkowski E, Hoppensteadt D, Walenga JM. Development of a flow cytometric assay for the laboratory diagnosis of heparin-induced thrombocytopenia. Blood 1996;88:317(Abst)
16. Visentin GP, Ford SE, Scott JP, Aster RH. Antibodies from patients with heparin-induced thrombocytopenia/thrombosis are specific for platelet factor 4 complexed with heparin or bound to endothelial cells. J Clin Invest 1994;93:81-88
17. 14C-serotonin release assay in the diagnosis of heparin-induced thrombocytopenia. Am J Clin Pathol 1995;104:648-654
18. Collins JL, Aster RH, Moghaddam M, et al. Diagnostic testing for heparin-induced thrombocytopenia: An enhanced platelet factor 4 complex enzyme-linked immunosorbent assay. Blood 1997;90:461(Abst)
19. Lindhoff-Last E, Stein M, Spiegel J, et al. Clinical relevance of heparin-platelet factor 4-antibodies in patients after vascular surgery. Ann Haematol 1997;74(Suppl II):A63
20. Amiral J, Marfaing-Koka A, Ponez M, Meyer D. The biological basis of immune heparin-induced thrombocytopenia. Platelets 1998;9:77-91
21. Amiral J, Marfaing-Koka A, Wolf M, et al. Presence of auto-antibodies to interleukin-8 or neutrophil activating peptide-2 in patients with heparin-associated-thrombocytopenia. Blood 1996; 88:410-116
22. Arkel YS, Ku DH, Larka J, Lam X. Comparison of the functional platelet aggregation and ATP release and heparin-plalelet factor 4 antibody ELISA methods in the testing for the heparin-induced thrombocytopenia syndrome. Blood 1997;90(Suppl I): 1096(Abst)
23. Walenga JM, Jeske WP, Fasanella AR, Wood J, Bakhos M. Laboratory tests for the diagnosis of heparin-induced thrombocytopenia. Semin Thromb Hemostas 1999(Supp I)43-50