Axonal and dendritic transport in Purkinje cells of cerebellar slice cultures studied by microinjection of horseradish peroxidase

Cell and Tissue Research

Volumn 295, Issue 1, 1999, Pages 55-64

  Krah, Kerstin ^a   Meller, Karl ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Antimitotic drugs; Axonal transport; Cytoskeleton; Dendritic transport; Microinjection; Organotypic culture; Purkinje cell; Rat

Indexed keywords

COLCHICINE; HORSERADISH PEROXIDASE; VINBLASTINE;

ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CEREBELLUM; DENDRITE; MICROINJECTION; NERVE FIBER TRANSPORT; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PURKINJE CELL; RAT; SYNAPSE;

ANIMALS; AXONAL TRANSPORT; AXONS; CEREBELLUM; CULTURE TECHNIQUES; DENDRITES; PEROXIDASE; PURKINJE CELLS; RATS;

EID: 0032895327     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004410051212     Document Type: Article

References (73)

1. Adams RJ, Bray D (1983) Rapid transport of foreign particles microinjected into crab axons. Nature 303:718-720
2. Allen RD, Weiss DG, Hayden JH, Brown DT, Fujiwake H, Simpson M (1985) Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: evidence for an active role of microtubules in cytoplasmic transport. J Cell Biol 100:1736-1752
3. Audinat E, Kn̈pfel T, G̈hwiler BH (1990) Responses to excitatory amino acids of Purkinje cells and neurones of the deep nuclei in cerebellar slice cultures. J Physiol (Lond) 430:297-313
4. Baas PW, Deitch JS, Black MM, Banker GA (1988) Polarity orientation of microtubules in hippocampal neurons: uniformity in the axon and nonuniformity in the dendrite. Proc Natl Acad Sci USA 85:8335-8339
5. Baas PW, Black MM, Banker GA (1989) Changes in microtubule polarity orientation during the development of hippocampal neurons in culture. J Cell Biol 109:3085-3094
6. Blank NK, Seil FJ (1982) Mature Purkinje cells in cerebellar tissue cultures: an ultrastructural study. J Comp Neurol 208:169-176
7. Brady ST (1985) A novel brain ATPase with properties expected for the fast axonal transport motor. Nature 317:73-75
8. Brady ST (1991) Molecular motors in the nervous system. Neuron 7:521-533
9. Brady ST, Lasek RJ, Allen RD (1985) Video microscopy of fast axonal transport in extruded axoplasm. Cell Motil 5:81-101
10. Broadwell RD, Olivier C, Brightman MW (1980) Neuronal transport of acid hydrolases and peroxidase within the lysosomal system of organelles. Involvement of agranular reticulum-like cisterns. J Comp Neurol 190:519-532
11. Burton PR (1988) Dendrites of mitral cell neurons contain microtubules of opposite polarity. Brain Res 473:107-115
12. Calvet M-C, Calvet J, Camacho R, Eude D (1983) The dendritic trees of Purkinje cells: a computer assisted analysis of HRP labeled neurons in organotypic cultures of kitten cerebellum. Brain Res 280:199-215
13. Cyr JL, Brady ST (1992) Molecular motors in axonal transport. Mol Neurobiol 6:137-155
14. Dusart I, Airaksinen MS, Sotelo C (1997) Purkinje cell survival and axonal regeneration are age dependent: an in vitro study. J Neurosci 17:3710-3726
15. Farrell KW, Wilson L (1984) Tubulin-colchicine complexes differentially poison opposite microtubule ends. Biochemistry 23:3741-3748
16. Fink BR, Byers MR, Middaugh ME (1973) Dynamics of colchicine effects on rapid axonal tansport and axonal morphology. Brain Res 56:299-311
17. G̈hwiler BH (1980) Morphological differentiation of nerve cells in thin organotypic cultures derived from rat hippocampus and cerebellum. Proc R Soc Lond Biol 211:287-290
18. G̈hwiler BH (1981) Organotypic monolayer cultures of nervous tissue. J Neurosci Methods 4:329-342
19. Goldberg DJ (1982) Microinjection into an identified axon to study the mechanism of fast axonal transport. Proc Natl Acad Sci USA 79:4818-4822
20. Graessmann M, Graessmann A (1986) Microinjection of tissue culture cells using glass capillaries: methods. In: Celis JE (ed) Microinjection and organelle transplantation techniques. Methods and applications. Academic Press, London, pp 3-13
21. Grafstein B, Forman DS (1980) Intracellular transport in neurons. Physiol Rev 60:1167-1283
22. Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291-302
23. Hammerschlag R, Cyr JL, Brady ST (1994) Axonal transport and the neuronal cytoskeleton. In: Siegel GJ (ed) Basic neurochemistry: molecular, cellular, and medical aspects. Raven, New York, pp 545-571
24. Haskins KM, Donoso JA, Himes RH (1981) Spirals and paracrystals induced by Vinca alkaloids: evidence that microtubule-associated proteins act as polycations. J Cell Sci 47:237-247
25. Hendelman WJ, Aggerwal AS (1980) The Purkinje neuron. I. A Golgi study of its development in the mouse and in culture. J Comp Neurol 193:1063-1079
26. Hendelman WJ, DeSavigny N, Marshall KC (1985) Growth and myelination of explant cultures in defined medium. In Vitro Cell Dev Biol 21:129-134
27. Hirokawa N (1996) The molecular mechanism of organelle transport along microtubules: the identification and characterization of Kifs (kinesin superfamily proteins). Cell Struct Funct 21:357-367
28. Hirokawa N, Bloom GS, Vallee RB (1985) Cytoskeletal architecture and immunocytochemical localization of microtubule-associated proteins in regions of axons associated with rapid axonal transport: the β,β'-iminodipropionitrile-intoxicated axon as a model system. J Cell Biol 101:227-239
29. Jäger CB, Kapoor R, Llinas R (1988) Cytology and organization of rat cerebellar organ cultures. Neuroscience 26:509-538
30. Koike H (1987) The disturbance of the fast axonal transport of protein by passive stretching of an axon in Aplysia. J Physiol (Lond) 390:489-500
31. Kristensson K, Olsson Y, Sjöstrand J (1971) Axonal uptake and retrograde transport of exogenous proteins in the hypoglossal nerve. Brain Res 32:399-406
32. Larcher J-C, Boucher D, Lazereg S, Gros F, Denoulet P (1996) Interaction of kinesin motor domains with a-and b-tubulin subunits at a tau-independent binding site. Am Soc Biochem Mol Biol 22117-22124
33. 3H-L-leucine. Brain Res 7:360-377
34. Lasek RJ (1970) Axonal transport of proteins in dorsal root ganglion cells of the growing cat: a comparison of growing and mature neurons. Brain Res 20:121-126
35. Lasek RJ, Brady ST (1982) The structural hypothesis of axonal transport: two classes of moving elements. In: Weiss DG (ed) Axoplasmic transport. Springer, Berlin Heidelberg New York, pp 397-405
36. Lasek R, Joseph BS, Whitlock DG (1968) Evaluation of a radioautographic tracing method. Brain Res 8:319-336
37. Li D, Field PM, Yoshioka N, Raisman G (1994) Axons regenerate with correct specificity in horizontal slice culture of the postnatal rat entorhino-hippocampal system. Eur J Neurosci 6:1026-1037
38. Lin SXH, Pfister KK, Collins CA (1996) Comparison of the intracellular distribution of cytoplasmic dynein and kinesin in cultured cells: motor protein location does not reliably predict function. Cell Motil Cytoskeleton 34:299-312
39. Linke R, Heimrich B, Frotscher M (1995) Axonal regeneration of identified septohippocampal projection neurons in vitro. Neuroscience 68:1-4
40. Maccioni RB, Cambiazo V (1995) Role of microtubule-associated proteins in the control of microtubule assembly. Physiol Rev 75:835-864
41. MacFarlane BV, Wright A, Benson HAE (1997) Reversible blockade of retrograde axonal transport in the rat sciatic nerve by vincristine. J Pharm Pharmacol 49:97-101
42. Meller K (1987) The cytoskeleton of cryofixed Purkinje cells of the chicken cerebellum. Cell Tissue Res 247:155-165
43. Meller K (1992) Axoplasmic transport of horseradish peroxidase in single neurons of the dorsal root ganglion studied in vitro by microinjection. Cell Tissue Res 270:139-148
44. Meller K (1994) Intracellular injection of transport markers and antibodies into in vitro cultured nerve cells. Neurosci Prot 50:1-10
45. Mercken M, Fischer I, Kosik KS, Nixon RA (1995) Three distinct axonal transport rates for tau, tubulin, and other microtubule-associated proteins: evidence for dynamic interactions of tau with microtubules in vivo. J Neurosci 15:8259-8267
46. Morris RL, Hollenbeck PJ (1995) Axonal transport of mitochondria along microtubules and f-actin in living vertebrate neurons. J Cell Biol 131:1315-1326
47. Mouginot D, Gähwiler BH (1995) Characterization of synaptic connections between cortex and deep nuclei of the rat cerebellum in vitro. Neuroscience 64:699-712
48. Na GC, Timasheff SN (1982) In vitro vinblastine-induced tubulin paracrystals. J Biol Chem 257:10387-10391
49. Nemhauser I, Goldberg DJ (1985) Structural effects in axoplasm of DNase I, an actin depolymerizer that blocks fast axonal transport. Brain Res 334:47-58
50. Notterpek LM, Bullock PN, Malek-Hedayat S, Fisher R, Rome LH (1993) Myelination in cerebellar slice cultures: development of a system amenable to biochemical analysis. J Neurosci Res 36:621-634
51. Ochs S, Burger E (1958) Movement of substances proximo-distally in the nerve axons as studied with spinal cord injections of radioactive phosphorus. Am J Physiol 194:499-506
52. Overly CC, Rieff HI, Hollenbeck PJ (1996) Organelle motility and metabolism in axons vs dendrites of cultured hippocampal neurons. J Cell Sci 109:971-980
53. Panda D, Jordan MA, Chin Chu K, Wilson L (1996) Differential effects of vinblastine on polymerization and dynamics at opposite microtubule ends. J Biol Chem 271:29807-29812
54. Paulson JC, McClure WO (1975) Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein. J Cell Biol 67:461-467
55. Price RL, Lasek RJ, Katz MJ (1991) Microtubules have special physical associations with smooth endoplasmic reticula and mitochondria in axons. Brain Res 540:209-216
56. Primi M-P, Clarke PGH (1997) Early retrograde effects of blocking axoplasmic transport in the axons of developing neurons. Dev Brain Res 99:259-262
57. Sato-Harada R, Okabe S, Umeyama T, Kanai Y, Hirokawa N (1996) Microtubule-associated proteins regulate microtubule function as the track for intracellular membrane organelle transports. Cell Struct Funct 21:283-295
58. Schnapp BJ, Reese TS (1989) Dynein is the motor for retrograde axonal transport of organelles. Proc Natl Acad Sci USA 86:1548-1552
59. Schnapp BJ, Vale RD, Sheetz MP, Reese TS (1985) Single microtubules from squid axoplasm support bidirectional movement of organelles. Cell 40:455-462
60. Schwartz JH, Goldberg DJ (1982) Studies on the mechanism of fast axoplasmic transport in single identified neurons. In: Weiss DG (ed) Axoplasmic transport. Springer, Berlin Heidelberg New York, pp 351-361
61. Seil FJ (1979) Cerebellum in tissue culture. Rev Neurosci 4:105-177
62. Seil FJ (1996) Neural plasticity in cerebellar cultures. Prog Neurobiol 50:533-556
63. Sharp DJ, Yu W, Baas PW (1995) Transport of dendritic microtubules establishes their nonuniform polarity orientation. J Cell Biol 130:93-103
64. Shpetner HS, Paschal BM, Vallee RB (1988) Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP1 C). J Cell Biol 107:1001-1009
65. Sternberger LA (1986) The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Sternberger LA (ed) Immunocytochemistry, 3rd edn. Wiley, New York, pp 90-209
66. Stoppini L, Buchs P-A, Muller D (1991) A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 37:173-182
67. Tanaka M, Tomita A, Yoshida S, Yano M, Shimizu H (1994) Observation of the highly organized development of granule cells in rat cerebellar organotypic cultures. Brain Res 641:319-327
68. Tauer U, Volk B, Heimrich B (1996) Differentiation of Purkinje cells in cerebellar slice cultures: an immunocytochemical and Golgi EM study. Neuropathol Appl Neurobiol 22:361-369
69. Toso RJ, Jordan MA, Farrell KW, Matsumoto B, Wilson L (1993) Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine. Biochemistry 32:1285-1293
70. Tytell M, Black MM, Garner JA, Lasek RJ (1981) Axonal transport: each major rate component reflects the movement of distinct macromolecular complexes. Science 214:179-181
71. Vale RD, Schnapp BJ, Reese TS, Sheetz MP (1985) Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon. Cell 40:449-454
72. Vandecandelaere A, Martin SR, Engelborghs Y (1997) Response of microtubules to the addition of colchicine and tubulin-colchicine: evaluation of models for the interaction of drugs with microtubules. Biochem J 323:189-196
73. Yu W, Baas PW (1994) Changes in microtubule number and length during axon differentiation. J Neurosci 14:2818-2829