Normalization of RNA hybridization signals by means of SYBR® green II- stained 28S or 18S ribosomal RNA and a phosphor imager

BioTechniques

Volumn 26, Issue 1, 1999, Pages 46-50

  Spiess, Andrej Nikolai ^a   Ivell, Richard ^a  

^a UNIVERSITY OF HAMBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; PHOSPHORUS; RNA 18S; RNA 28S;

ANIMAL TISSUE; ARTICLE; FLUORESCENCE; IMAGING SYSTEM; MOUSE; NONHUMAN; NORTHERN BLOTTING; RNA HYBRIDIZATION; SIGNAL PROCESSING; TECHNIQUE;

ANIMALIA;

EID: 0032894221     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99261bm06     Document Type: Article

References (10)

1. Barbu, V. and F. Dautry. 1989. Northern blot normalization with a 28S rRNA oligonucleotide probe. Nucleic Acids Res. 17:7115.
2. Bonini, J.A. and C. Hofmann. 1991. A rapid, accurate, nonradioactive method for quantitating RNA on agarose gels. BioTechniques 11:708-709.
3. Correa-Rotter, R., C.N. Mariash and M.E. Rosenberg. 1992. Loading and transfer control for Northern hybridization. BioTechniques 12:154-158.
4. de Leeuw, W.J.F., P.E. Slagboom and J. Vijg. 1989. Quantitative comparison of mRNA levels in mammalian tissues: 28S ribosomal RNA level as an accurate internal control. Nucleic Acids Res. 17:10137-10138.
5. Duhl, D.M., D.D. Gillespie and F. Sulser. 1992. Ethidium bromide fluorescence of 28S ribosomal RNA can be used to normalize samples in Northern or dot blots when analyzing small drug-induced changes in specific mRNA. J. Neurosci. Methods 42:211-218.
6. Feinberg, A.P. and B. Vogelstein. 1983. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
7. Kerkhof, L. 1997. Quantification of total RNA by ethidium bromide fluorescence may not accurately reflect the RNA mass. J. Biochem. Biophys. Methods 34:147-154.
8. Molecular Probes. 1996. Handbook of Fluorescent Probes. Leiden, The Netherlands.
9. Molecular Probes. 1997. SYBR Green II Information Leaflet. Leiden, The Netherlands.
10. Wade, M.F. and J.L. O'Conner. 1992. Using a cationic carbocyanine dye to assess RNA loading in Northern gel analysis. BioTechniques 12:794-796.