Cardenolide variation within and among natural populations of Digitalis obscura

Journal of Plant Physiology

Volumn 154, Issue 4, 1999, Pages 426-430

  Nebauer, Sergio G ^a   Del Castillo Agudo, Lucas ^a   Segura, Juan ^a  

^a UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Cardenolide inter and intra populational variation; Digitalis obscura L.; Glycoside hydrolysis; HPLC; Scrophulariaceae; Willow leaved foxglove

Indexed keywords

DIGITALIS OBSCURA;

EID: 0032892316     PISSN: 01761617     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0176-1617(99)80278-8     Document Type: Article

References (23)

1. CASTRO BRAGA, F., W. KREIS, R. ALMEIDA RECIO, and A. BRAGA DE OLIVEIRA: Variation of cardenolides with growth in a Digitalis lanato brazilian cultivat. Photochemistry 45, 473-476 (1997).
2. DICOSMO, F. and M. MISAWA: Plant cell and tissue culture: alternatives for metabolite production. Biotechnology Advances 13(3), 425-453 (1995).
3. GÄRTNER, D. E. and H. U. SEITZ: Enzyme activities in cardenolide accumulating, mixotropic shoot cultures of Digitalis purpurea L. J. Plant Physiol. 141, 269-275 (1993).
4. GAVIDIA, I.: Influencia del genotipo, condiciones de cultivo y radiación gamma sobre la morfogénesis y producción de cardenólidos en Digitalis obscura L. Ph. D. Thesis, University of Valencia, Spain, 1996.
5. GAVIDIA, I., L. DEL CASTILLO AGUDO, and P. PÉREZ-BERMÚDEZ: Selection and long term cultures of high-yielding Digitalis obscura plants: RAPD markers for analysis of genetic stability. Plant Sci. 121, 197-205 (1996).
6. GAVIDIA, I. and P. PÉREZ-BERMÚDEZ: Cardenolides of Digitalis obscura: the effect of phosphate and manganese on growth and productivity of shoot-tip cultures. Phytochemistry 45, 81-85 (1997).
7. HARBOURNE, J. B. and B. L. TURNER: Plant Chemosystematics. Academic Press, London, UK, 1984.
8. HARTMANN, T.: Diversity and variability of plant secondary metabolism: a mechanistic view. Entomol. Exp. Appl. 80, 177-188 (1996).
9. HAYWARD, M. D. and N. R. SACKVILLE HAMILTON: Genetic diversity-population structure and conservation. In: CALLOW, J. A., B. V. FORD-LLOYD, and H. J. NEWBURY (eds.): Biotechnology and Plant Genetic Resources, pp. 49-76. CAB International, New York, USA (1997).
10. HOAGLAND, D. R. and D. I. ARNON: The water culture method for growing plants without soil. Calif. Agric. Exp. Stn., Circular 347 (1950).
11. HOELZ, H., W. KREIS, B. HAUG, and E. REINHARD: Storage of cardiac glycosides in vacuoles of Digitalis lanata mesophyll cells. Phytochemistry 31, 1167-1171 (1992).
12. KREIS, W. and U. MAY: Cardenolide glucosyltransferases and glucohydrolases in leaves and cell cultures of three Digitalis (Scrophulariaceae) species. J. Plant Physiol. 136, 247-252 (1990).
13. LICHIUS, J. J.: Phytochemische Analyse seltener Digitalisarten (wie Digitalis subalpina Br.-Bl.) und reziproker Digitaliskreuzungen. J. Cramer, Berlin, Germany, 1991.
14. LUCKNER, M. and B. DIETTRICH: Cardenolides. In: CONSTABEL, F. and I. K. VASIL (eds.): Cell Culture and Somatic Cell Genetics of Plants, vol. 5, pp. 193-212. Academic Press, San Diego (1988).
15. LUI, J. H. C. and E. J. STABA: Effects of age and growth regulators on serially propagated Digitalis lanata leaf and root cultures. Planta Medica 41, 90-95 (1981).
16. SCHÖNER, S. and E. REINHARD: Long-term cultivation of Digitalis lanata clones propagated in vitro: cardenolide content of the regenerated plants. Planta Medica 52, 478-481 (1986).
17. SOKAL, R. R. and F. J. ROHLF: Biometry. Freeman and Company, New York, USA, 1995.
18. STUHLEMMER, U. and W. KREIS: Cardenolide formation and activity of pregnane-modifying enzymes in cell suspension cultures, shoot cultures and leaves of Digitalis lanata. Plant Physiol. Biochem. 34(1), 85-91 (1996).
19. 2 enrichment and water stress. Phytochemistry 26, 2735-2739 (1987).
20. WEILER, E. W. and P. WESTEKEMPER: Rapid selection of strains of Digitalis lanata EHRH. with high digoxin content. Planta Medica 35, 316-322 (1979).
21. WICHTL, M., W. BÜHL, and G. HUESMANN: Fingerhut. Digitalis L. - bekannte und weniger bekannte Vertreter einer wichtigen Arzneipflanzengattung. Deutsche Apotheker Ztg. 127, 2391-2400 (1987).
22. WICHTL, M., W. WICHTL-BLEIER, and M. MANGKUDIDJOJO: Hochleistungs-Flüssigkeits-Chromatographische Analyse von Digitalis-Blattextrakten J. Chromatography 247, 359-365 (1982).
23. WIEGREBE, H. and M. WICHTL: High-performance liquid chromatographic determination of cardenolides in Digitalis leaves after solid-phase extraction. J. Chromatography 630, 402-407 (1993).