Protease-resistant prion protein produced in vitro lacks detectable infectivity

Journal of General Virology

Volumn 80, Issue 1, 1999, Pages 11-14

  Hill, Andrew F ^a   Antoniou, Michael ^b   Collinge, John ^a,c  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

^b GUY S HOSPITAL   (United Kingdom)

^c ST MARY S HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PRION PROTEIN; PROTEINASE;

ARTICLE; PRIORITY JOURNAL; TRANSGENIC MOUSE; VIRUS INFECTIVITY; VIRUS PATHOGENESIS;

CRICETINAE; MUS MUSCULUS;

EID: 0032892306     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-80-1-11     Document Type: Article

References (23)

1. Antoniou, M. (1991). Induction of erythroid-specific expression in murine erythroleukaemia (MEL) cell lines. In Methods in Molecular Biology, vol. 7, Gene Transfer and Expression Protocols, 1st edn, pp. 421-433. Edited by E. J. Murray. Clifton. NJ: Humana Press.
2. Bessen, R. A., Kocisko, D. A., Raymond, G. J., Nandan, S., Lansbury, P. T. & Caughey, B. (1995). Non-genetic propagation of strain-specific properties of scrapie prion protein. Nature 375, 698-700.
3. Bueler, H., Aguzzi, A., Sailer, A., Greiner, R. A., Autenried, P., Aguet, M. & Weissmann, C. (1993). Mice devoid of PrP are resistant to scrapie. Cell 73, 1339-1347.
4. Caughey, B., Raymond, G. J., Kocisko, D. A. & Lansbury, P. T., Jr (1997). Scrapie infectivity correlates with converting activity, protease resistance, and aggregation of scrapie-associated prion protein in guanidine denaturation studies. Journal of Virology 71, 4107-4110.
5. Collinge, J., Palmer, M. S., Sidle, K. C. L., Gowland, I., Medori, R., Ironside, J. & Lantos, P. L. (1995). Transmission of fatal familial insomnia to laboratory animals. Lancet 346, 569-570.
6. Collinge, J., Sidle, K. C. L., Meads, J., Ironside, J. & Hill, A. F. (1996). Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD. Nature 383, 685-690.
7. Hsiao, K. K., Groth, D., Scott, M., Yang, S.-L., Serban, H., Rapp, D., Foster, D., Torchia, M., DeArmond, S. J. & Prusiner, S. B. (1994). Serial transmission in rodents of neurodegeneration from transgenic mice expressing mutant prion protein. Proceedings of the National Academy of Sciences, USA 91, 9126-9130.
8. Hsiao, K. K., Scott, M., Foster, D., Groth, D. F., DeArmond, S. J. & Prusiner, S. B. (1990). Spontaneous neurodegeneration in transgenic mice with mutant prion protein. Science 250, 1587-1590.
9. Kascsak, R. J., Rubenstein, R., Merz, P. A., Tonna DeMasi, M., Fersko, R., Carp, R. I., Wisniewski, H. M. & Diringer, H. (1987). Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. Journal of Virology 61, 3688-3693.
10. Kocisko, D. A., Come, J. H., Priola, S. A., Chesebro, B., Raymond, G. J., Lansbury, P. T. & Caughey, B. (1994). Cell-free formation of protease-resistant prion protein. Nature 370, 471-474.
11. Kocisko, D. A., Priola, S. A., Raymond, G. J., Chesebro, B., Lansbury, P. T., Jr & Caughey, B. (1995). Species specificity in the cell-free conversion of prion protein to protease-resistant forms: a model for the scrapie species barrier. Proceedings of the National Academy of Sciences, USA 92, 3923-3927.
12. Lasmezas, C. I., Destys, J.-P., Robain, O., Jaegly, A., Beringue, V., Peyrin, J.-M., Fournier, J.-G., Hauw, J.-J., Rossier, J. & Dormont, D. (1997). Transmission of the BSE agent to mice in the absence of detectable abnormal prion protein. Science 275, 402-405.
13. Needham, M., Egerton, M., Millest, A., Evans, S., Popplewell, M., Cerillo, G., McPheat, J., Monk, A., Jack, A., Johnstone, D. and others (1995). Further development of the locus control region/murine erythroleukemia expression system: high level expression and characterization of recombinant human calcitonin receptor. Protein Expression and Purification 6, 124-131.
14. Pan, K.-M., Baldwin, M., Nguyen, J., Gasset, M., Serban, A., Groth, D., Mehlhorn, I., Huang, Z., Fletterick, R. J., Cohen, F. E. & Prusiner, S. B. (1993). Conversion of α-helices into β-sheets features in the formation of the scrapie prion proteins. Proceedings of the National Academy of Sciences, USA 90, 10962-10966.
15. Prusiner, S. B. (1987). Bioassays of prions. In Prions: Novel Infectious Pathogens causing Scrapie and Creutzfeldt-Jakob Disease, pp. 65-81. Edited by S. B. Prusiner & M. P. McKinley. San Diego: Academic Press.
16. Prusiner, S. B. (1991). Molecular biology of prion diseases. Science 252, 1515-1522.
17. Prusiner, S. B., Bolton, D. C., Groth, D. F., Bowman, K., Cochran, S. P. & McKinley, M. P. (1982). Further purification and characterization of scrapie prions. Biochemistry 21, 6942-6950.
18. Scott, M., Foster, D., Mirenda, C., Serban, D., Coufal, F., Wälchli, M., Torchia, M., Groth, D., Carlson, G., DeArmond, S. J., Westaway, D. & Prusiner, S. B. (1989). Transgenic mice expressing hamster prion protein produce species-specific scrapie infectivity and amyloid plaques. Cell 59, 847-857.
19. Scott, M. R., Kohler, R., Foster, D. & Prusiner, S. B. (1992). Chimeric prion protein expression in cultured cells and transgenic mice. Protein Science 1, 986-997.
20. Scott, M., Groth, D., Foster, D., Torchia, M., Yang, S. L., DeArmond, S. J. & Prusiner, S. B. (1993). Propagation of prions with artificial properties in transgenic mice expressing chimeric PrP genes. Cell 73, 979-988.
21. Telling, G. C., Parchi, P., DeArmond, S. J., Cortelli, P., Montagna, P., Gabizon, R., Mastrianni, J., Lugaresi, E., Gambetti, P. & Prusiner, S. B. (1996). Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity. Science 274, 2079-2082.
22. Weissmann, C. (1991). Spongiform encephalopathies. The prion's progress. Nature 349, 569-571.
23. Xi, Y. G., Ingrosso, L., Ladogana, A., Masullo, C. & Pocchiari, M. (1992). Amphotericin B treatment dissociates in vivo replication of the scrapie agent from PrP accumulation. Nature 356, 598-601.