The HPr(Ser) kinase of Streptococcus salivarius: Purification, properties, and cloning of the hprK gene

Journal of Bacteriology

Volumn 181, Issue 3, 1999, Pages 709-717

  Brochu, Denis ^a   Vadeboncoeur, Christian ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL ENZYME; DODECYL SULFATE SODIUM; FRUCTOSE BISPHOSPHATE; GUANIDINE; MAGNESIUM; PHOSPHATE; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE; PROTEIN SERINE KINASE;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; CHROMATOFOCUSING; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STREPTOCOCCUS SALIVARIUS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA; STREPTOCOCCUS SALIVARIUS;

EID: 0032891071     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.3.709-717.1999     Document Type: Article

References (48)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1997. Current protocols in molecular biology. Greene Publishing and Wiley Interscience, New York, N.Y.
2. Chou, P. W., and G. D. Fasman. 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47:45-148.
3. Deutscher, J., and R. Engelmann. 1984. Purification and characterization of an ATP-dependent protein kinase from Streptococcus faecalis. FEMS Microbiol. Lett. 23:157-162.
4. Deutscher, J., and M. H. Saier, Jr. 1983. ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes. Proc. Natl. Acad. Sci. USA 80:6790-6794.
5. Deutscher, J., U. Kessler, and W. Hengstenberg. 1985. Streptococcal phosphoenolpyruvate:sugar phosphotransferase system: purification and characterization of a phosphoprotein phosphatase which hydrolyzes the phosphoryl bond in seryl-phosphorylated histidine-containing protein J. Bacteriol. 163: 1203-1209.
6. Deutscher, J., E. Küster, I. Bergstedt, V. Charrier, and W. Hillen. 1995. Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in gram-positive bacteria. Mol. Microbiol. 15: 1049-1053.
7. Deutscher, J., J. Reizer, C. Fischer, A. Gatinier, M. H. Saier, Jr., and M. J. Steinmetz. 1994. Loss of protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis. J. Bacteriol. 176:3336-3344.
8. Duclos, B., S. Marcandier, and A. J. Cozzone. 1991. Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis. Methods Enzymol. 201:10-21.
9. Ferrari, S., and G. Thomas. 1991. Micro-and macropurification methods for protein kinases. Methods Enzymol. 200:159-169.
10. Fujita, Y., Y. Miwa, A. Galinier, and J. Deutscher. 1995. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive clement by a protein complex formed between CcpA and seryl-phosphorylated HPr. Mol. Microbiol. 17:953-960.
11. Fukui, K., K. Kato, T. Kodama, H. Ohta, T. Shimamoto, and T. Shimono. 1988. Kinetic study of a change in intracellular ATP level associated with aerobic catabolism of ethanol by Streptococcus mutans. J. Bacteriol. 170: 4589-4593.
12. Galinier, A., M. Kravanja, R. Engelman, W. Hengstenberg, M. C. Kilhoffer, J. Deutscher, and J. Haiech. 1998. New protein kinase and protein phosphatase families mediate signal transduction in bacterial catabolite repression. Proc. Natl. Acad. Sci. USA 95:1823-1828.
13. Garnier, J., and B. Robson. 1989. In G. D. Fasman (ed.). Prediction of protein structure and the principles of protein conformation, p. 417-466. Plenum, New York, N.Y.
14. Gauthier, L., S. Thomas, G. Gagnon, M. Frenette, L. Trahan, and C. Vadeboncoeur. 1994. Positive selection for resistance to 2-deoxyglucose gives rise, in Streptococcus salivarius, to seven classes of pleiotropic mutants, including ptsH and ptsl missense mutants. Mol. Microbiol. 13:1101-1109.
15. Gauthier, M., D. Brochu, L. D. Eltis, S. Thomas, and C. Vadehoncoeur. 1997. Replacement of isoleucine-47 by threonine in the HPr protein of Streptococcus salivarius abrogates the preferential metabolism of glucose and fructose over lactose and melibiose but does not prevent the phosphorylation of HPr on serine-46. Mol. Microbiol. 25:695-705.
16. Genetics Computer Group Inc. 1996. Wisconsin Package, version 9.0. Genetics Computer Group. Inc., Madison, Wis.
17. Gorbach, S. L. 1990. Lactic acid bacteria and human health. Ann. Med. 22:37-41.
18. Gösseringer, R., E. Küster, A. Galinier, J. Deutscher, and W. Hillen. 1997. Cooperative and non-cooperative DNA binding modes of catabolite control protein CcpA from Bacillus megaterium result from sensing two different signals. J. Mol. Biol. 266:665-676.
19. Hamilton, I. R. 1968. Synthesis and degradation of intracellular polyglucose in Streptococcus salivarius. Can. J. Microbiol. 14:65-77.
20. Hamilton, I. R. 1987. Effect of changing environment on sugar transport and metabolism by oral bacteria, p. 94-133. In J. Reizer and A. Peterkofsky (ed.), Sugar transport and metabolism by Gram-positive bacteria. Ellis Horwood, Chichester, England.
21. Hamilton, I. R. 1990. Maintenance of proton motive force by Streptococcus mutans and Streptococcus sobrinus during growth in continuous culture. Oral Microbiol. Immunol. 5:280-287.
22. Hueck, C. J., and W. Hillen. 1995. Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the Gram-positive bacteria? Mol. Microbiol. 15:395-401.
23. Hueck, C. J., W. Hillen, and M. H. Saier, Jr. 1994. Analysis of a cis-active sequence mediating catabolite repression in Gram-positive bacteria. Res. Microbiol. 145:503-518.
24. Iwami, Y., T. Yamada, and S. Araya. 1975. Glycolytic intermediates in Streptococcus mutans PK1. Arch. Oral Biol. 20:695-697.
25. Jones, B. E., V. Dossonnet, E. Küster, W. Hillen, J. Deutscher, and R. E. Klevit. 1997. Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr. J. Biol. Chem. 272:26530-26535.
26. Kashket, S., and E. R. Kashket. 1985. Dissipation of the proton motive force in oral streptococci by fluoride. Infect. Immun. 48:19-22.
27. Krüger, S., S. Gertz, and M. Hecker. 1996. Transcriptional analysis of bglH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression. J. Bacteriol. 178:2637-2644.
28. Küster, E., E. J. Luesink, W. M. de Vos, and W. Hillen. 1996. Immunological crossreactivity to the catabolite control protein CcpA trom Baccillus megaterium is found in many Gram-positive bacteria. FEMS Microbiol. Lett. 139: 109-115.
29. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
30. Marshall, V. M. 1487. Lactic acid bacteria: starters for flavour. FEMS Microbiol. Rev. 46:327-336.
31. Martin-Verstraete, I., J. Stülke, A. Klier, and G. Rapoport. 1995. Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon. J. Bacteriol. 177:6919-6927.
32. Meadow, N. D., D. K. Fox, and S. Roseman. 1990. The bacterial phosphoenolpyruvate:glycose phosphotransferase system. Annu. Rev. Biochem. 59:497-542.
33. Postma, P. W., J. W. Lengeler, and G. R. Jacobson. 1993. Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria. Microbiol Rev. 57:543-594.
34. Reizer, J., C. Hoischen, F. Titgemeyer, C. Rivolta, R. Rabus, J. Stülke, D. Karamata, M. H. Saier, Jr., and W. Hillen. 1998. A novel protein kinase that controls carbon catabolite repression in bacteria. Mol. Microbiol. 27:1157-1169.
35. Reizer, J., M. J. Novotny, W. Hengstenberg, and M. H. Saier, Jr. 1984. Properties of ATP-dependent protein kinase from Streptococcus pyogenes that phosphorylates a servi residue in HPr, a phosphocarrier protein of the phosphotransferase system. J. Bacteriol. 160:333-3411.
36. Saier, M. H., Jr., and J. Reizer. 1992. Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate sugar phosphotransferase system. J. Bacteriol. 174:1433-1438.
37. Saier, M. H., Jr., S. Chauvaux, G. M. Cook, J. Deutscher, I. T. Paulsen, J. Reizer, and J. J. Ye. 1996. Catabolite repression and inducer control in Gram-positive bacteria Microbiology 142:217-230.
38. Salyers, A. A., and D. D. Whitt. 1994. Bacterial pathogenesis: a molecular approach. ASM Press, Washington, U.C.
39. Sambrook, J. E., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
40. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
41. Simpson, C. L., and R. B. B. Russell. 1998. Identification of a homolog of CcpA catabolite repressor protein in Streptococcus mutans. Infect. Immun. 66:2085-2092.
42. Thevenot, T., D. Brochu, C. Vadeboncoeur, and I. R. Hamilton. 1995. Regulation of ATP-dependent P-(Scr)-HPr formation in Streptococcus mutans and Streptococcus salivarius. J. Bacteriol. 177:2751-2759.
43. Vadeboncoeur, C., and M. Pelletier. 1997. The phosphoenolpyruvate:sugar phosphotransferase system of oral streptococci and its role in the control of sugar metabolism. FEMS Microbiol. Rev. 19:187-207.
44. Vadeboncoeur, C., D. Brochu, and J. Reizer. 1991. Quantitative determination of the intracellular concentration of the various forms of HPr, a phosphocarrier protein of the phosphocnolpuruvate:sugar phosphotransferase system in growing cells of oral streptococci. Anal. Biochem. 196:24-30.
45. Rodrigue, L., L. Lacoste, L. Trahan, and C. Vadeboncoeur. 1988. Effect of nutritional constraint on the biosynthesis of the components of the phosphoeolpyruate:sugar phosphotransferase system in a fresh isolate of Streptococcus mutans. Infect. Immun. 56:518-522.
46. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vector and host strains: nucleotide sequences of the m13mp18 and pUC19 vector. Gene 33:103-119.
47. Ye, J. J., J. Reizer, and M. H. Saier, Jr. 1994. Regulation of 2-deoxyglucose phosphate accumulation in Lactococcus lactis vesicles by metabolite-activated, ATP-dependent phosphorylation of serine-46 in HPr of the phosphotransferase system. Microbiology 140:3421-3429.
48. + symport in Lactobacillus brevis. Proc. Natl. Acad. Sci. USA 91:3102-3106.