Enterovirus detection from wastewater by RT-PCR and cell culture

Water Science and Technology

Volumn 40, Issue 2, 1999, Pages 105-109

  Gantzer, C ^a   Senouci, S ^a   Maul, A ^a   Levi Y ^b   Schwartzbrod, L ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

^b CIRSEE   (France)

Author keywords

Cell culture; Enterovirus; RT PCR; Wastewater

Indexed keywords

BIOMARKERS; CELL CULTURE; CONTAMINATION; ENVIRONMENTAL IMPACT; ENVIRONMENTAL PROTECTION; SAMPLING; VIRUSES; WATER QUALITY;

ENTEROVIRUS DETECTIONS; GENOMES;

WASTEWATER TREATMENT;

GLASS;

ADSORPTION; CELL CULTURE; CONFERENCE PAPER; ENTEROVIRUS; GENOME; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CONCENTRATION; VIRUS INFECTION; VIRUS ISOLATION; WASTE WATER TREATMENT PLANT;

EID: 0032883489     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0273-1223(99)00437-0     Document Type: Conference Paper

References (15)

1. Abbaszadegan, M, Huber, M. S., Gerba, C. P. and Pepper, I. L. (1993). Detection of enteroviruses in groundwater with the polymerase chain reaction. Appl. Environ. Microbiol., 59, 1318-1324.
2. Atmar, R. L., Neill, F. H., Romalde, J. L., Le Guyader, F., Woodley, C. M., Metcalf, T. G. and Estes, M. K. (1995). Detection of Norwalk virus and hepatitis A virus in shellfish tissue with PCR. Appl. Environ Microbiol., 61, 3014-3018.
3. Chomczynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
4. Deng, M. Y., Day, S. P. and Cliver, D. O. (1994). Detection of hepatitis A virus in environmental sample by antigen capture PCR. Appl. Environ. Microbiol., 60, 1927-1933.
5. Gantzer, C., Senouci, S., Maul, A., Levi, Y. and Schwartzbrod, L. (1997). Enterovirus genomes in wastewater: concentration on glass wool and glass powder and detection by RT-PCR. J. Virol. Meth., 65, 265-271.
6. Kopecka, H., Dubrou, S., Prevot, J., Marechal, J. and Lopez-Pila, J. M. (1993). Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction and hybridization. Appl. Environ. Microbiol., 59, 1213-1219.
7. Le Guyader, F., Menard, D., Pommepuy, M and Kopecka, H. (1995). Use of RT semi-nested PCR to assess viral contamination in caribbean rivers (Martinique). Wat. Sci. Tech., 31(5-6), 391-398.
8. Leparc, I., Fuchs, F., Kopecka, H. and Aymard, M. (1993). Use of the polymerase chain reaction with a murine model of Picornavirus induced myocarditis. J. Clin. Microbiol., 31, 2890-2894.
9. Lucena, F., Finance, C., Jofre, J., Sancho, J. and Schwartzbrod, L. (1982). Viral pollution determination of superficial waters (river water and seawater from the urban area of Barcelona, Spain). Wat. Res., 16, 173-177.
10. Puig, M., Jofre, J., Lucena, F., Allard, A., Wadell, G. and Girones, R. (1994), Detection of adenoviruses and enteroviruses in polluted waters by nested PCR amplification. Appl. Environ. Microbiol., 60, 2963-2970.
11. Schwab, K. J., De Leon ,R. and Sobsey, M. D. (1993). Development of PCR methods for enteric virus detection in water. Wat. Sci. Tech., 27(3-4), 211-218.
12. Schwartzbrod, L. and Lucena-Gutierrez, F. (1978). Concentration des entérovirus dans les eaux par adsorption sur poudre de verre: proposition d'un appareillage simplifié. Microbia, 44, 55-58.
13. Schwartzbrod, L., Vilagines, P., Schwartzbrod, J., Sarrette, B., Vilagines, R. and Collomb, J. (1985). Evaluation of the viral population in two wastewater treatment plants. Wat. Res., 19, 1353-1356.
14. Vilagines, P., Sarrette, B., Husson, G. and Vilagines, R. (1992). Définition des conditions optimales d'adsorption du poliovirus type 1 sur de la laine de verre sodocalcique : application à des eaux de distribution et des eaux résiduaires. J. Fr. Hydrol., 23(1), 101-107.
15. Vilagines, P., Sarrette, B., Husson, G. and Vilagines, R. (1993), Glass wool for virus concentration at ambient water pH level. Wat. Sci. Tech., 27(3-4), 299-306.