Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an enzyme- linked immunosorbent assay

Journal of Clinical Microbiology

Volumn 37, Issue 11, 1999, Pages 3475-3480

  Ikadai, Hiromi ^a   Xuan, Xuenan ^a   Igarashi, Ikuo ^a   Tanaka, Shigeyasu ^b   Kanemaru, Takumi ^c   Nagasawa, Hideyuki ^a   Fujisaki, Kozo ^a   Suzuki, Naoyoshi ^a   Mikami, Takeshi ^a  

^a OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^b SHIZUOKA UNIVERSITY   (Japan)

^c EQUINE RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; RECOMBINANT PROTEIN;

ARTICLE; BABESIA; BABESIA CABALLI; DNA LIBRARY; ENZYME LINKED IMMUNOSORBENT ASSAY; MEROZOITE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PARASITE IDENTIFICATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS;

EID: 0032882604     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.11.3475-3480.1999     Document Type: Article

References (30)

1. Avarzed, A., I. Igarashi, T. Kanemaru, K. Hirumi, Y. Omata, A. Saito, T. Oyamada, H. Nagasawa, Y. Toyoda, and N. Suzuki. l997. Improved in vitro cultivation of Babesia caballi. J. Vet. Med. Sci. 59:479-481.
2. Böse, R., and K. Deaman. 1992. Demonstration of the humoral immune response of horses to Babesia caballi by Western blotting. Int. J. Parasitol. 22:627-630.
3. Böse, R., and B. Peymann. 1994. Diagnosis of Babesia caballi infections in horses by enzyme-linked immunosorbent assay (ELISA) and Western blot. Int. J. Parasitol. 24:341-346.
4. Böse, R., B. Peymann, and P. Barbosa. 1994. Identification of diagnostic antigens for South American Babesia caballi infections. Int. J. Parasitol. 24:255-258.
5. Brüning, A., P. Phipps, E. Posnett, and E. U. Canning. 1997. Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis. Vet. Parasitol. 58:11-26.
6. Dalrymple, B. P., R. E. Casu, J. M. Peters, C. M. Dimmock, K. R. Gale, R. Boese, and I. G. Wright. 1993. Characterization of a family of multi-copy genes encoding rhoptry protein homologues in Babesia bovis, Babesia ovis, and Babesia canis. Mol. Biochem. Parasitol. 57:181-192.
7. Dalrymple, B. P., J. M. Peters, R. Böse, and I. G. Wright. 1996. A polymerase chain-reaction method for the identification of genes encoding members of the Bv60/p58 family of rhoptry protein homologues in the genus Babesia. Exp. Parasitol. 84:96-100.
8. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
9. Friedhoff, K. T. 1982. The piroplasms of equidae. Significance for the international equine trade. Berl. Muench. Tieraertzl. Wochenschr. 95:368-374.
10. Honda, Y., M. Waithaka, E. L. N. Taracha, L. Duchateau, A. J. Musoke, and D. J. McKeever. 1998. Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection. Vaccine 16: 1276-1282.
11. Ikadai, H., S. Kabamoto, X. Xuan, I. Igarashi, H. Nagasawa, K. Fujisaki, N. Suzuki, and T. Mikami. Protein analysis of Babesia caballi merozoites from in vitro culture by two-dimensional polyacrylamide gel electrophoresis and Western blotting. Submitted for publication.
12. Ikadai, H., Y. Tamaki, X. Xuan, I. Igarashi, S. Kawai, H. Nagasawa, K. Fujisaki, Y. Toyoda, N. Suzuki, and T. Mikami. Inhibitory effect of monoclonal antibodies on the growth of Babesia caballi. Int. J. Parasitol., in press.
13. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
14. McElwain, T. F., L. E. Perryman, W. C. Davis, and T. C. McGuire. 1987. Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites. J. Immunol. 138:2298-2304.
15. McElwain, T. F., L. E. Perryman, A. J. Musoke, and T. C. McGuire. 1991. Molecular characterization and immunogenicity of neutralization-sensitive Babesia bigemina merozoite surface proteins. Mol. Biochem. Parasitol. 47: 213-222.
16. Mishra, V. S., T. F. McElwain, J. B. Dame, and E. B. Stephens. 1992. Isolation, sequence, and differential expression of the p58 gene family of Babesia bigemina. Mol. Biochem. Parasitol. 53:149-158.
17. Mishra, V. S., E. B. Stephens, J. B. Dame, L. E. Perryman, T. C. McGuire, and T. F. McElwain. 1991. Immunogenicity and sequence analysis of recombinant p58: A neutralization-sensitive, antigenically conserved Babesia bigemina merozoite surface protein. Mol. Biochem. Parasitol. 47:207-212.
18. Palmer, G. H., T. F. McElwain, L. E. Perryman, W. C. Davis, D. R. Reduker, D. P. Jasmer, V. Shkap, E. Pipano, W. L. Goff, and T. C. McGuire. 1991. Strain variation of Babesia bovis merozoite surface-exposed epitopes. Infect. Immun. 59:3340-3342.
19. Purnell, R. E. 1981. Babesiosis in various hosts, p. 25-64. In M. Ristic and J. P. Krier (ed.), Babesiosis. Academic Press, Inc., New York, N.Y.
20. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
21. Sam-Yellowe, T. Y. 1996. Rhoptry organelles of the apicomplexa: their role in host cell invasion and intracellular survival. Parasitol. Today 12:308-316.
22. Schein, E. 1988. Equine babesiosis, p. 197-208. In M. Ristic (ed.), Babesiosis of domestic animals and man. CRC Press, Inc., Boca Raton, Fla.
23. Short, J. M., J. A. Sorge, and W. D. Huse. 1988. λZAP: a bacteriophage with in vivo excision properties. Nucleic Acids Res. 16:7583-7600.
24. Smith, D. B., and K. S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 67:31-40.
25. Suarez, C. E., G. H. Palmer, D. P. Jasmer, S. A. Hines, L. E. Perryman, and T. F. McElwain. 1991. Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface-exposed epitopes. Mol. Biochem. Parasitol. 46:45-52.
26. Tanaka, S., T. Yora, K. Nakayama, K. Inoue, and K. Kurosumi. 1997. Proteolytic processing of pro-opiomelanocortin occurs in acidifying secretory granules of AtT-20 cells. J. Histochem. Cytochem. 45:425-436.
27. Tenter, A. M., and K. T. Friedhoff. 1986. Serodiagnosis of experimental and natural Babesia equi and B. caballi infections. Vet. Parasitol. 20:49-61.
28. Weiland, G. 1986. Species-specific serodiagnosis of equine piroplasma infections by means of complement fixation test, immunofluorescence, and enzyme-linked immunosorbent assay. Vet. Parasitol. 20:43-48.
29. Wright, I. G., R. Casu, M. A. Commins, B. P. Dalrymple, K. R. Gale, B. V. Goodger, P. W. Riddles, D. J. Waltisbuhl, I. Abetz, D. A. Berrie, Y. Bowles, C. Dimmock, T. Hayes, H. Kalnins, G. Leatch, R. McCrae, P. E. Montague, I. T. Nisbet, F. Parrodi, J. M. Peters, P. C. Scheiwe, W. Smith, K. Rode-Bramanis, and M. A. White. 1992. The development of a recombinant Babesia vaccine. Vet. Parasitol. 44:3-13.
30. Yasuda, A., J. Kimura-Kuroda, M. Ogimoto, T. Sata, T. Sato, C. Takamura, T. Kurata, A. Kojima, and K. Yasui. 1990. Induction of protective immunity in animals vaccinated with recombinant vaccinia viruses that express pre M and E glycoproteins of Japanese encephalitis virus. J. Virol. 64:2788-2795.