Quantification of mycelium of Botrytis spp. and the antagonist Ulocladium atrum in necrotic leaf tissue of cyclamen and lily by fluorescence microscopy and image analysis

Phytopathology

Volumn 89, Issue 10, 1999, Pages 868-876

  Kessel, G J T ^a   De Haas, B H ^a   Lombaers Van Der Plas, C H ^a   Meijer, E M J ^a   Dewey, F M ^b   Goudriaan, J ^a   Van Der Werf, W ^a   Kohl J ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

B. elliptica; Botrytis cinerea; Interfungal competition; Microbial ecology; Sampling strategy

Indexed keywords

FLUORESCENCE MICROSCOPY; FUNGUS DETECTION; IMAGE ANALYSIS; MYCOSIS; PLANT PATHOGEN INTERACTION;

BOTRYOTINIA FUCKELIANA; BOTRYTIS; BOTRYTIS ELLIPTICA; CYCLAMEN; CYCLAMEN PERSICUM; FUNGI; LILIUM; TRITICUM AESTIVUM; ULOCLADIUM ATRUM;

EID: 0032880920     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.1999.89.10.868     Document Type: Article

References (35)

1. Adee, S. R., Pfender, W. F., and Hartnett, D. C. 1990. Competition between Pyrenophora tritici-repentis and Septoria nodorum in the wheat leaf as measured with de Wit replacement series. Phytopathology 80:1177-1182.
2. Bermingham, S., Dewey, F. M., and Maltby, L. 1995. Development of a monoclonal antibody-based immunoassay for the detection and quantification of Anguillospora longissima colonizing leaf material. Appl. Environ. Microbiol. 61:2606-2613.
3. Biles, C. L., and Hill, J. P. 1988. Effect of Trichoderma harzianum on sporulation of Cochliobolus sativas on excised wheat seedling leaves. Phytopathology 78:656-659.
4. Bossi, R., and Dewey, F. M. 1992. Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea. Plant Pathol. 41:472-482.
5. Chou, M. C., and Preece, T. F. 1968. The effect of pollen grains on infections caused by Botrytis cinerea Fr. Ann. Appl. Biol. 62:11-22.
6. Cole, L., Dewey, F. M., and Hawes, C. R. 1996. Infection mechanisms of Botrytis species: Pre-penetration and pre-infection processes of dry and wet conidia. Mycol. Res. 100(3):277-286.
7. Daniel, O., Schönholzer, F., and Zeyer, J. 1995. Quantification of fungal hyphae in leaves of deciduous trees by automated image analysis. Appl. Environ. Microbiol. 61:3910-3918.
8. Delesse, M. A. 1847. Procédé mécanique pour déterminer la composition des roches. C. R. Acad. Sci. (Paris) 25:544-545.
9. de Waard, M. A., Georgopoulos, S. G., Hollomon, D. W., Ihsii, H., Leroux, P., Ragsdale, N. N., and Schwinn, F. J. 1993. Chemical control of plant diseases: Problems and prospects. Annu. Rev. Phytopathol. 31:403-421.
10. Dewey, F. M., Twiddy, D. R., Phillips, S. I., Grose, M. J., and Wareing, P. W. 1992. Development of a quantitative monoclonal antibody-based immunoassay for Humicola lanuginosa on rice grains and comparison with conventional assays. Food Agric. Immunol. 4:153-167.
11. Doss, R. P., Chastagner, G. A., and Riley, K. L. 1986. Screening ornamental lilies for resistance to Botrytis elliptica. Sci. Hortic. 30:237-246.
12. Elad, Y., Köhl, J., and Fokkema, N. J. 1994. Control of infection and sporulation of Botrytis cinerea on bean and tomato by saprophytic yeasts. Phytopathology 84:1193-1200.
13. Goudriaan, J., and van Laar, H. H. 1994. Modelling Potential Crop Growth Processes. Pages 95-110 in: Current Issues in Production Ecology. Vol. 2. Kluwer Academic Publishers, Dordrecht, the Netherlands.
14. Gullino, M. L. 1992. Chemical control of Botrytis spp. Pages 217-222 in: Recent Advances in Botrytis Research. K. Verhoeff, N. E. Malathrakis, and B. Williamson, eds. Pudoc Scientific Publishers, Wageningen, the Netherlands.
15. Harrison, J. G., Barker, H., Lowe, R., and Rees, E. A. 1990. Estimation of amounts of Phytophthora infestans by enzyme-linked immunosorbent assay. Plant Pathol. 39:274-277.
16. Hicks, R. E., and Newell, S. Y. 1983. An improved gas chromatographic method for measuring glucosamine and muramic acid concentrations. Anal. Biochem. 128:438-445.
17. Hu, J., Nazar, R. N., and Robb, J. 1993. Quantification of Verticillium biomass in wilt disease development. Physiol. Mol. Plant Pathol. 42:23-26.
18. Karpovich-Tate, N., Spanu, P., and Dewey, F. M. 1998. Use of monoclonal antibodies to determine biomass of Cladosporium fulvum in infected tomato leaves. Mol. Plant-Microbe Interact. 11:710-716.
19. Köhl, J., Bélanger, R. R., and Fokkema, N. J. 1997. Interaction of four antagonistic fungi with Botrytis aclada in dead onion leaves: A comparative microscopic and ultrastructural study. Phytopathology 87:634-642.
20. Köhl, J., Gerlagh, M., De Haas, B. H., and Krijger, M. C. 1998. Biological control of Botrytis cinerea in cyclamen with Ulocladium atrum and Gliocladium roseum under commercial growing conditions. Phytopathology 88:568-575.
21. Köhl, J., Molhoek, W. M. L., van der Plas, C. H., and Fokkema, N. J. 1995. Suppression of sporulation of Botrytis spp. as a valid biocontrol strategy. Eur. J. Plant Pathol. 101:251-259.
22. Köhl, J., Molhoek, W. M. L., van der Plas, C. H., and Fokkema, N. J. 1995. Effect of Ulocladium atrum and other antagonists on sporulation of Botrytis cinerea on dead lily leaves exposed to field conditions. Phytopathology 85:393-401.
23. Köhl, J., van der Plas, C. H., Molhoek, W. M. L., and Fokkema, N. J. 1995. Effect of interrupted leaf wetness periods on suppression of sporulation of Botrytis allii and Botrytis cinerea by antagonists on dead onion leaves. Eur. J. Plant Pathol. 101:627-637.
24. Lumsden, R. D., Carter, J. P., Whipps, J. M., and Lynch, J. M. 1990. Comparison of biomass and viable propagule measurements in the antagonism of Trichoderma harzianum against Pythium ultimum. Soil Biol. Biochem. 22:187-194.
25. Morrell, J. J., Gibson, D. G., and Krahmer, R. L. 1985. Effect of fluorescent-labeled lectins on visualization of decay fungi in wood sections. Phytopathology 75:329-332.
26. Newton, M. R., Kinkel, L. L., and Leonard, K. J. 1997. Competition and density-dependent fitness in a plant parasitic fungus. Ecology 78(6): 1774-1784.
27. Pfender, W. F., King, L. G., and Rabe, J. R. 1991. Use of dual-stain fluorescence microscopy to observe antagonism of Pyrenophora tritici-repentis by Limonomyces roseipellis in wheat straw. Phytopathology 81:109-112.
28. Plasencia, J., Jemmerson, R., and Banttari, E. E. 1996. Production and characterization of monoclonal antibodies to Verticillium dahliae and development of a quantitative immunoassay for fungal biomass. Phytopathology 86:170-176.
29. Ricker, R. W., Marois, J. J., Dlott, J. W., Bostock, R. M., and Morrison, J. C. 1991. Immunodetection and quantification of Botrytis cinerea on harvested wine grapes. Phytopathology 81:404-411.
30. Ride, J. P., and Drysdale, R. D. 1972. A rapid method for chemical estimation of filamentous fungi in plant tissue. Physiol. Plant Pathol. 2:7-15.
31. Seitz, L. M., Sauer, D. B., Burroughs, R., Mohr, H. E., and Hubbard, J. D. 1979. Ergosterol as a measure of fungal growth. Phytopathology 69:1202-1203.
32. Sokal, R. R., and Rohlf, F. J. 1995. Pages 309-320 in: Biometry: The Principles and Practice of Statistics in Biological Research. 3rd ed. W. H. Freeman and Co., New York.
33. Trese, A. T., and Loschke, D. C. 1990. High contrast resolution of the mycelia of pathogenic fungi in corn tissue after staining with calcofluor and destaining with cellulase. Phytopathology 80:196-200.
34. van de Plas, P., and Leunissen, J. L. M. 1993. Ultrasmall gold probes: Characteristics and use in immuno(cyto)chemical studies. Pages 241-257 in: Methods in Cell Biology. Vol. 37, Antibodies in Cell Biology. J. D. Asai, ed. Academic Press, London.
35. Weibel, E. R. 1979. Stereological Methods. Vol. 1, Practical Methods for Biological Morphometry. Academic Press, London.