A single-chain fusion molecule consisting of peptide, major histocompatibility gene complex class I heavy chain and β2-microglobulin can fold partially correctly, but binds peptide inefficiently

Scandinavian Journal of Immunology

Volumn 50, Issue 4, 1999, Pages 355-362

  Sylvester Hvid, C ^a   Nielsen, L L B ^a   Hansen, N J V ^a   Pedersen, L O ^a   Buus, S ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BETA 2 MICROGLOBULIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANTIGEN PRESENTING CELL; ARTICLE; BACTERIUM ISOLATE; BINDING AFFINITY; CYTOTOXIC T LYMPHOCYTE; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; GENETIC ANALYSIS; IMMUNE RESPONSE; MAJOR HISTOCOMPATIBILITY COMPLEX; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING;

ANTIGEN PRESENTATION; BETA 2-MICROGLOBULIN; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; MODELS, IMMUNOLOGICAL; NICKEL; NITRILOTRIACETIC ACID; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FOLDING; RECEPTORS, ANTIGEN, T-CELL; RECOMBINANT FUSION PROTEINS; SEPHAROSE;

EID: 0032879342     PISSN: 03009475     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3083.1999.00601.x     Document Type: Article

References (28)

1. Germain RM, Margulies DH. The biochemistry and cell biology of antigen processing and presentation. Annu Rev Immunol 1993;11:403-50.
2. Yewdell JW, Bennick JR. Cell biology of antigen procssing and presentation to major histocompatibility complex class I molecule-restricted T lymphocytes. Adv Immunol 1995;52:1-123.
3. Folk K, Rotzschke O, Stevanovic S, Jung G, Rammensee H-G. Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules. Nature 1991;351:290-6.
4. Boyd LF, Kozlowski S, Margulies DH. Solution binding of an antigenic peptide to a major histocompatibility complex class I molecule and the role of b2-micoglobulin. Proc Natl Acad Sci USA 1992;89:2242-6.
5. Ruppert J, Sidney J, Celis E, Kubo RT, Sette A. Prominent role of secondary anchor residues in peptide binding to HLA-A2.1 molecules. Cell 1993;74:929-37.
6. Stryhn A, Pedersen LØ, Romme T, Holm CB, Holm A, Buns S. Peptide binding specificity of major histocompatibility complex class I resolved into on array of apparently independent sub-specificities: quantitation by peptide libraries and improved prediction of binding. Eur J Immunol 1996;26:1911-8.
7. Olsen AC, Pedersen LØ, Hansen AS et al. A quantitative assay to measure the interaction between immunogenic peptides and MHC class I molecules. Eur J Immunol 1994;24:385-92.
8. Stryhn A, Pedersen LØ, Romme T et al. pH dependence of MHC Class I-restricted peptide presentation. J Immunol 1996;156:4191-7.
9. Garboezi DN, Hung DT, Wiley DC. HLA-A2-peptide complexes: Refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides. Proc Natl Acad Sci USA 1992;89:3429-33.
10. Madden DR, Garboczi DN, Wiley DC. The antigenic identity of peptide-MHC complexes: a comparison of the conformations of five viral peptides presented by HLA-A2. Cell 1993;75:693-708.
11. Parker KC, Carreno BM, Sestak L, Utz U, Biddison WE, Coligan JE. Peptide binding to HLA-A2 and HLA-B27 isolated from Escherichia coli. Reconstitution of HLAA2 HLA-B27 heavy chain/beta-2-microglobulin complexes requires specific peptides. J Biol Chem 1992;267:5451-9.
12. Moestrup SK, Holtet TL, Etzerodt M et al. α2-macroglobulin: proteinase, plasminogen activator complexes and receptor-associated protein bind to a region of the a2-macroglobulin receptor containing a cluster of eighth complement-type repeats. J Biol Chem 1993;268:13691-6.
13. Pedersen LØ, Stryhn A, Holtet TL et al. The interaction of beta 2-microglobulin (β2m) with mouse class I major histocompatibility antigens and its ability to support peptide binding. A comparison of human and mouse β2m. Eur J Immunol 1995;25:1609-16.
14. Mottez E, Langlade-Demoyen P, Gournier H et al. Cells expressing a major histocompatibility complex class I molecule with a single covalently bound peptide are highly immunogenic. J Exp Med 1995;181:493-502.
15. Laemmli UK. Clevage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970;227:680-5.
16. Tonkonogy SL, Amos DB. Paired H-2-specific monoclonal antibodies react diffferently to red cells and lymphocytes. Immunogenetics 1981;14:497-505.
17. Lemke H, Hämmerling GJ. Topographic arrangement of H-2 determinants defined by monoclonal hybridoma antibodies. In: Hämmerling G, Hämmerling U, Kearney JF, eds. Monoclonal Antibodies and T Cell Hybridomas. North Holland: Elsevier, 1981:102-9.
18. Buus S, Sette A, Colon SM, Jenis DM, Grey HM. Isolation and characterization of antigen-Ia complexes involved in T cell recognition. Cell 1986;47:1071-7.
19. Buus S, Stryhn A, Winther K, Kirkby N, Pedersen LØ. Receptor-ligand interactions measured by an improved spun column chromatography technique. A high efficiency and high throughput size separation method. Biochim Biophys Acta 1995;1243:453-60.
20. Sweetser MT, Morrison LA, Brachiale VL, Brachiale TJ. Recognition of pre-processed endogenous antigen by class I, but not class II, MHC restricted T cells. Nature 1989;342:180-2.
21. Stryhn A, Pedersen LØ, Ortiz-Navarrete V, Buus S. Preformed purified peptide/MHC class I complexes arc potent stimulators of class I-restricted T cell hybridomas. Eur J Immunol 1994;24:1404-9.
22. Schumacher TNM, De Bruijn MLH, Vernie LN et al. Peptide selection by MHC class I molecules. Nature 1991;350:703-6.
23. Fremont DH, Matsumura M, Stura EA, Peterson PA, Wilson IA. Crystal structure of two viral peptides in complex with murine MHC class I H-2Kb. Science 1992;257:919-27.
24. Holtet TL. Biochemical and structural studies of selected multi domain proteins. PhD thesis. University of Aarhus, 1995.
25. Parker KC, Wiley DC. Overexpression of native human beta 2-microglobulin in Escherichia coli and its purification. Gene 1989;83:117-24.
26. Andersen PS, Stryhn A, Hansen BE, Fugger L, Engberg J, Buus S. A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells. Proc Natl Acad Sci USA 1996;93:1820-4.
27. Stryhn A, Andersen PS, Pedersen LØ et al. Shared fine specificity between T-cell receptors and an antibody recognizing a peptide/major histocompatibility complex class I complex. Proc Natl Acad Sci USA 1996;93:10338-42.
28. Collins EJ, Garboczi DN, Wiley DC. Three-dimensional structure of a peptide extending from one end of a class I MHC binding site. Nature 1994;371:626-9.