Identification of O-antigen polymerase transcription and translation start signals and visualization of the protein in Salmonella enterica serovar Typhimurium

Microbiology

Volumn 145, Issue 9, 1999, Pages 2443-2451

  Wong, Danny K H ^a   Morris, Christina ^a   Lam, Tin L ^a   Wong, Wan K R ^a   Hackett, Jim ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

Lipopolysaccharide; Rfc; Semi rough; Wzy

Indexed keywords

GLUTATHIONE TRANSFERASE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI K 12; IMMUNOBLOTTING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; SALMONELLA ENTERICA; SALMONELLA TYPHIMURIUM; SEQUENCE ANALYSIS; TRANSCRIPTION INITIATION; TRANSLATION INITIATION;

ESCHERICHIA COLI; SALMONELLA ENTERICA; SALMONELLA TYPHIMURIUM;

EID: 0032879212     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-145-9-2443     Document Type: Article

References (36)

1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (1993). Current Protocols in Molecular Biology, vol. 2, pp. 11.2.1-11.2.5. New York: John Wiley.
2. Batchelor, R. A., Haraguchi, G. E., Hull, R. A. & Hull, S. I. (1991). Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli. J Bacteriol 173, 5699-5704.
3. Brown, P. K., Romana, L. K. & Reeves, P. R. (1992). Molecular analysis of the rfb gene cluster of Salmonella serovar muenchen (strain M67): the genetic basis of the polymorphism between groups C2 and B. Mol Microbiol 6, 1385-1394.
4. Cerin, H. & Hackett, J. (1989). Molecular cloning and analysis of the incompatibility and partition functions of the virulence plasmid of Salmonella typhimurium. Microb Pathog 7, 85-99.
5. Collins, L. V. & Hackett. J. (1991). Molecular cloning, characterization, and nucleotide sequence of the rfc gene, which encodes an O-antigen polymerase of Salmonella typhimurium. J Bacteriol 173, 2521-2529.
6. Coyne, M. J., Jr & Goldberg, J. B. (1995). Cloning and characterization of the gene (rfc) encoding O-antigen polymerase of Pseudomonas aeruginosa PAO1. Gene 167, 81-86.
7. Curd, H., Liu, D. & Reeves, P. R. (1998). Relationships among the O-antigen gene clusters of Salmonella enterica groups B, D1, D2, and D3. J Bacteriol 180, 1002-1007.
8. Daniels, C., Vindurampulle, C. & Morona, R. (1998). Over-expression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy). Mol Microbiol 28, 1211-1222.
9. De Kievit, T. R., Dasgupta, T., Schweizer, H. & Lam, J. S. (1995). Molecular cloning and characterization of the rfc gene of Pseudomonas aeruginosa (serotype 05). Mol Microbiol 16, 565-574.
10. Farinha, M. A. & Kropinski, A. M. (1990). Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters. J Bacteriol 172, 3496-3499.
11. Grosjean, H. & Fiers, W. (1982). Preferential codon usage in prokaryotic genes: the optimal codon-anti-codon energy and the selective codon usage in efficiently expressed genes. Gene 18, 199-209.
12. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580.
13. Hawley, D. K. & McClure, W. R. (1983). Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res 11, 2237-2255.
14. Hitchcock, P. J. & Brown, T. M. (1983). Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154, 269-277.
15. Hopp, T. P., Prickett, K. S., Price, V. L., Libby, R. T., March, C. J., Cerretti, D. P., Urdal, D. L. & Conlon, P. J. (1998). A short polypeptide marker sequence useful for recombinant protein identification and purification. Biotechnology 6, 1204-1210.
16. Klena, J. D. & Schnaitman, C. A. (1993). Function of the rfb gene cluster and the rfe gene in the synthesis of O antigen by Shigella dysenteriae 1. Mol Microbiol 9, 393-402.
17. Lerner, C. G. & Inouye, M. (1990). Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability. Nucleic Acids Res 18, 463.
18. Liu, D. & Reeves, P. R. (1994). Escherichia coli K12 regains its O antigen. Microbiology 140, 49-57.
19. Lukomski, S., Hull, R. A. & Hull, S. I. (1996). Identification of the O antigen polymerase (rfc) gene in Escherichia coli O4 by insertional mutagenesis using a nonpolar chloramphenicol resistance cassette. J Bacteriol 178, 240-247.
20. McGrath, B. C. & Osborn, M. J. (1991). Localization of the terminal steps of O-antigen synthesis in Salmonella typhimurium. J Bacteriol 173, 649-654.
21. Mäkelä, P. H. (1965). Inheritance of the O antigens of Salmonella groups B and D. J Gen Microbiol 41, 57-65.
22. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbour, NY: Cold Spring Harbour Laboratory.
23. Morona, R., Mavris, M., Fallarino, A. & Manning, P. A. (1994). Characterization of the rfc region of Shigella fiexneri. J Bacteriol 176, 733-747.
24. Naide, Y., Nikaido, H., Mäkelä, P. H., Wilkinson, R. G. & Stocker, B. A. D. (1965). Semirough strains of Salmonella. Proc Natl Acad Sci USA 53, 147-153.
25. Neidhardt, F. C. & Umbarger, H. E. (1996). Chemical composition of Escherichia coli. In Escherichia coli and Salmonella: Cellular and Molecular Biology, pp. 13-16. Washington, DC: American Society for Microbiology.
26. Norrander, J. M., Kempe, T. & Messing, J. (1983). Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26, 101-106.
27. Nurminen, M., Hellerqvist, C. E., Valtonen, V. V. & Mäkelä, P. H. (1971). The smooth lipopolysaccharide character of 1,4,(5),12 and 1,9,12 transductants formed as hybrids between groups B and D of Salmonella. Eur J Biochem 22, 500-505.
28. Phillips, T. A., Van Bogelen, R. A. & Neidhardt, F. C. (1984). Lon gene product of Escherichia coli is a heat-shock protein. J Bacteriol 159, 283-287.
29. Reeves, P. R., Hobbs, M., Valvano, M. A. & 8 other authors. (1996). Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol 4, 495-503.
30. Rosenberg, M. & Court, D. (1979). Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13, 319-353.
31. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
32. Smith, D. B. & Johnson, K. S. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase. Gene 67, 31-40.
33. Xiang, S. H., Hobbs, M. & Reeves, P. R. (1994). Molecular analysis of the rfb gene cluster of a group D2 Salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group E and D1 strains. J Bacteriol 176, 4357-4365.
34. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.
35. Yao, Z. & Valvano, M. A. (1994). Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4-α. J Bacteriol 176, 4133-4143.
36. Zhang, L., Toivanen, P. & Skurnik, M. (1996). The gene cluster directing antigen biosynthesis in Yersinia enterocolitica serotype O:8: identification of the genes for mannose and galactose biosyntheses and the gene for the O-antigen polymerase. Microbiology 142, 277-288.