A method for the separation of mouse pancreatic islets using discontinuous Percoll gradient centrifugation

Journal of Microbiology and Biotechnology

Volumn 9, Issue 4, 1999, Pages 522-524

  Cho, Yu Ree ^a   Kim, Sangduk ^a   Chang, Hyo Ihl ^a   Sung, Ha Chin ^a   Lee, Cherl Ho ^a   Kim, Chan Wha ^a  

^a Korea University   (South Korea)

Author keywords

Gradient centrifugation; Osmolality; Pancreatic islet; Percoll

Indexed keywords

ANIMAL CELL; ARTICLE; CELL ISOLATION; CENTRIFUGATION; CONTROLLED STUDY; MOUSE; NONHUMAN; OSMOLALITY; PANCREAS ISLET CELL;

EID: 0032878663     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Baetens, D., F. Malisse-Lage, A. Perrelet, and L. Orci. 1979. Endocrine pancreas: Three-dimensional reconstruction shows two types of Langerhans. Science 206: 1323-1324.
2. Field, J., A. Farney, and D. E. R. Sutherland. 1996. Improved islet isolation from rat pancreas using 35% bovine serum albumin in combination with dextran gradient separation. Transplantation 61: 1554-1556.
3. Gray, D. W., R. McShane, A. Grant, and P. L. Morris. 1984. A method for isolation of islets of Langerhans from the human pancreas. Diabetes 33: 1055-1061.
4. Lacy, P. E. and M. Kostianovsky. 1967. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35-39.
5. Lacy, P. E., E. T. Lacy, E. H. Finke, and Y. Yasunami. 1982. An improved method for the isolation of islets from the deer pancreas. Diabetes 31: 109-112.
6. Ricordi, C., C. Socci, A. M. Davalli, C. Staudacher, P. Baro, A. Verova, I. Sassi, F. Gavazzi, G. Pozza, and V. D. Carlo. 1990. Isolation of the elusive pig islet. Surgery 107: 688-694.
7. Shin, J.-S., H.-I. Chang, H.-C. Sung, and C.-W. Kim. 1999. The effect of fetal bovine serum, epidermal growth factor, and retinoic acid on rat islets embedded in collagen gels. J. Microbiol. Biotechnol. 9: 150-156.
8. Van Suylichem, P. T. R., G. H. J. Wolters, and R. van Schilfgaarde. 1989. The efficacy of density gradients for islet purification. Transplantation Proceedings 21: 2624-2625.
9. Vonen, B., J. Florholmen, A. K. Giever, and P. G. Burhol. 1987. A methodological study of discontinuous Percoll-gradient separation of pancreatic islets from rats. Scand. J. Lab. Invest. 47: 415-420.
10. Warnock, L. G., D. Ellis, R. V. Rajotte, I. Dawidson, S. Baekkeskov, and J. Egebjerg. 1988. Studies of the isolation and viability of human islets of Langerhans. Transplantation 45: 9957-9963.
11. Yamamoto, T., T. Asano, A. Mori, C. Kumai, M. Okumura, and T. Soji. 1981. A rapid method for the separation of rat pancreatic islets from collagenase-digested pancreas using Percoll. Endocrinol. Jpn. 28: 563-567.