High level expression of Thermococcus litoralis 4-α-glucanotransferase in a soluble form in Escherichia coli with a novel expression system involving minor arginine tRNAs and GroELS

FEBS Letters

Volumn 457, Issue 3, 1999, Pages 393-396

  Imamura, Hiromi ^a   Jeon, Beong Sam ^a   Wakagi, Takayoshi ^a   Matsuzawa, Hiroshi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

4 Glucanotransferase; GroELS; Rare codon; Recombinant protein; Thermococcus litoralis; Transfer RNA

Indexed keywords

ARGININE TRANSFER RNA; RECOMBINANT PROTEIN;

ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; THERMOCOCCUS;

CODON; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLYCOGEN DEBRANCHING ENZYME SYSTEM; GROEL PROTEIN; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; REPLICATION ORIGIN; RNA, TRANSFER, ARG; SOLUBILITY; THERMOCOCCUS;

EID: 0032878235     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(99)01081-9     Document Type: Article

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