Rat tumour necrosis factor-α: Expression in recombinant Pichia pastoris, purification, characterization and development of a novel ELISA

European Cytokine Network

Volumn 10, Issue 3, 1999, Pages 383-392

  Rees, Gwen S ^a   Gee, Chris K ^a   Ward, Helen L ^a   Ball, Christina ^a   Tarrant, Gill M ^a   Poole, Stephen ^a   Bristow, Adrian F ^a  

^a NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

ELISA; Expression vectors; Recombinant cytokines; Tumour necrosis factor

Indexed keywords

AMMONIUM SULFATE; DISULFIDE; ENDOTOXIN; INTERLEUKIN 1; INTERLEUKIN 6; METHIONINE; POLYCLONAL ANTIBODY; RECOMBINANT TUMOR NECROSIS FACTOR ALPHA; SEPHADEX;

ACETYLATION; AMINO ACID SEQUENCE; ARTICLE; CROSS REACTION; CYTOLYSIS; CYTOTOXICITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FRACTIONATION; GENE EXPRESSION SYSTEM; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PROTEIN PURIFICATION; RAT; SPECIES DIFFERENCE; YEAST;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MALE; MASS SPECTROMETRY; PICHIA; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0032878101     PISSN: 11485493     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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