Cytokeratin-negative desmoplastic small round cell tumor: A report of two cases emphasizing the utility of reverse transcriptase-polymerase chain reaction

Modern Pathology

Volumn 12, Issue 9, 1999, Pages 849-853

  Trupiano, Jacqueline K ^a   Machen, S Karen ^a   Barr, Frederic G ^b   Goldblum, John R ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Cytokeratin; Desmin; Desmoplastic small round cell tumor; Epithelial membrane antigen; Molecular genetics

Indexed keywords

CYTOKERATIN; DESMIN; EPITHELIAL MEMBRANE ANTIGEN; NEURON SPECIFIC ENOLASE; SYNAPTOPHYSIN; VIMENTIN;

ADULT; ARTICLE; BIOPSY; CANCER COMBINATION CHEMOTHERAPY; CASE REPORT; CLINICAL FEATURE; DESMOPLASTIC SMALL ROUND CELL TUMOR; FEMALE; HUMAN; IMMUNOHISTOCHEMISTRY; MALE; MOLECULAR GENETICS; MORTALITY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ABDOMINAL NEOPLASMS; ADULT; CARCINOMA, SMALL CELL; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; KERATINS; MALE; ONCOGENE PROTEINS, FUSION; PHOSPHOPYRUVATE HYDRATASE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SYNAPTOPHYSIN; TUMOR CELLS, CULTURED; VIMENTIN;

EID: 0032877878     PISSN: 08933952     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Gerald WL, Rosai J. Desmoplastic small cell tumor with divergent differentiation. Pediatr Pathol 1989;9:177-83.
2. Gerald WL, Miller HK, Battifora H, Miettinen M, Silva EG, Rosai J. Intra-abdominal desmoplastic small round-cell tumor. Report of 19 cases of a distinctive type of high-grade polyphenotypic malignancy affecting young individuals. Am J Surg Pathol 1991;15:499-513.
3. Ordonez NG. Desmoplastic small round cell tumor. II: an ultrastructural and immunohistochemical study with emphasis on new immunohistochemical markers. Am J Surg Pathol 1998;22:1314-27.
4. Gerald WL, Ladanyi M, de Alava E, Cuatrecasas M, Kushner BH, LaQuaglia MP, et al. Clinical, pathologic, and molecular spectrum of tumors associated with t(11;22)(p13;q12): desmoplastic small round-cell tumor and its variants. J Clin Oncol 1998;16:3028-36.
5. Rodriguez E, Sreekantaiah C, Gerald W, Reuter VE, Motzer RJ, Shaganti RSK. A recurring translocation, t(11;22)(p13; q11.2), characterizes intra-abdominal desmoplastic small cell tumors. Cancer Genet Cytogenet 1993;69:17-21.
6. Gerald WL, Rosai J, Ladanyi M. Characterization of the genomic breakpoint and chimeric transcripts in the EWS-WT1 gene fusion of desmoplastic small round cell tumor. Proc Natl Acad Sci U S A 1995;92:1028-32.
7. Ladanyi M, Gerald W. Fusion of the EWS and WT1 genes in the desmoplastic small round cell tumor. Cancer Res 1994; 54:2837-40.
8. Brodie SG, Stocker SJ, Wardlaw JC, Duncan MH, McConnell TS, Feddersen RM, et al. EWS and WT1 gene fusion in desmoplastic small round cell tumor of the abdomen. Hum Pathol 1995;26:1370-4.
9. Argatoff LH, O'Connell JX, Mathers JA, Gilks CB, Sorensen PH. Detection of the EWS/WT1 gene fusion by reverse transcriptase-polymerase chain reaction in the diagnosis of intra-abdominal desmoplastic small round cell tumor. Am J Surg Pathol 1996;20:406-12.
10. de Alava E, Ladanyi M, Rosai J, Gerald WL. Detection of chimeric transcripts in desmoplastic small round cell tumor and related developmental tumors by reverse transcriptase polymerase chain reaction. A specific assay. Am J Pathol 1995;147:1584-91.
11. Barr FG, Xiong QB, Kelly K. A consensus polymerase chain reaction-oligonucleotide hybridization approach for the detection of chromosomal translocations in pediatric bone and soft tissue cancers. Am J Clin Pathol 1995;104:627-33.
12. Barr FG, Chatten J, D'Cruz CM, Wilson AE, Nauta LE, Nycum LM, et al. Molecular assays for chromosomal translocations in the diagnosis of pediatric soft tissue sarcomas. JAMA 1995;273;553-7.
13. Antonescu DR, Gerald WL, Magid MS, Ladanyi M. Molecular variants of the EWS-WT1 gene fusion in desmoplastic small round cell tumor. Diagn Mol Pathol 1998;7:24-8.
14. Ordonez NG. Desmoplastic small round cell tumor. I: a histopathologic study of 39 cases with emphasis on unusual histological patterns. Am J Surg Pathol 1998;22:1303-13.
15. Dorsey BV, Benjamin LE, Rauscher F, Klencke B, Venook AP, Warren RS, et al. Intra-abdominal desmoplastic small round-cell tumor: expansion of the pathologic profile. Mod Pathol 1996;9:703-9.
16. Katz RL, Quezado M, Senderowicz AM, Villalba L, Laskin WB, Tsokos M. An intra-abdominal small round cell neoplasm with features of primitive neuroectodermal and desmoplastic round cell tumor and a EWS/FLI-1 fusion transcript. Hum Pathol 1997;28:502-9.
17. Ordi J, de Alava E, Torné A, Mellado B, Pardo-Mindan J, Iglesias X, et al. Intraabdominal desmoplastic small round cell tumor with EWS/ERG fusion transcript. Am J Surg Pathol 1998;22:1026-32.