A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability

Theoretical and Applied Genetics

Volumn 99, Issue 5, 1999, Pages 772-784

  Cannell, M E ^a   Doherty, A ^a   Lazzeri, P A ^a   Barcelo, P ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Marker gene stability; Transgene inheritance; Transgenic wheat; Triticum aestivum; Tritordeum

Indexed keywords

GENE EXPRESSION; GENETIC STABILITY; GENETIC TRANSFORMATION; HERITABILITY; INHERITANCE; PLANT GENETICS; TRANSGENIC PLANT;

TRITICUM AESTIVUM; TRITORDEUM;

EID: 0032875863     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001220051296     Document Type: Article

References (22)

1. Barcelo P, Lazzeri PA (1995) Transformation of cereals by microprojectile bombardment of immature inflorescence and scutellum tissues. In: Jones H (ed) Methods in molecular biology, vol. 49: plant gene transfer and expression protocols. Humana Press, Totowa, N.J., pp 113-123
2. Barro F, Cannell ME, Lazzeri PA, Barcelo P (1998) The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants. Theor Appl Genet 97:684-695
3. Callis J, Fromm ME, Walbot V (1987) Introns increase gene expression in cultured maize cells. Genes Dev 1:1183-1200
4. Cheng M, Fry JE, Pang S, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol 115:971-980
5. Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5:213-218
6. Cooley J, Ford T, Christou P (1995) Molecular and genetic characterization of elite transgenic rice plants produced by electric-discharge particle acceleration. Theor Appl Genet 90:97-104
7. Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organisation in rice engineered through direct DNA transfer supports a two phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci USA 95:7203-7208
8. Kumpatla SP, Hall TC (1998) Recurrent onset of epigentic silencing in rice harboring a multicopy transgene. Plant J 14:129-135
9. Kumpatla SP, Teng W, Bucholz WG, Hall TC (1997) Epigenetic transcriptional silencing and 5-azacytidine mediated reactivation of a complex transgene in rice. Plant Physiol 115:361-373.
10. Kumpatla SP, Chandrasekharan MB, Iyer LM, Li G, Hall TC (1998) Genome intruder scanning and modulation systems and transgene silencing. Trends Plant Sci 3:97-104
11. McElroy D, Zhang W, Ca J, Wu R (1990) Isolation of and efficient Actin promoter for use in rice transformation. Plant Cell 2:163-171
12. Park Y-D, Papp I, Moscone EA, Iglesias VA, Vaucheret H, Matzke AJM, Matzke MA (1996) Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity. Plant J 9:183-194
13. Pawlowski WP, Somers DA (1996) Transgene inheritance in plants genetically engineered by microprojectile bombardment. Mol Biotechnol 6:17-30
14. Pawlowski WP, Somers DA (1998) Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA. Proc Natl Acad Sci USA 95:12106-12110
15. Pijnacker LP, Ferwerda MA (1994) Sister chromatid exchanges in cultured immature embryos of wheat species and regenerants. Theor Appl Genet 89:267-292
16. Register JC, Peterson DJ, Bell PJ, Bullock WP, Evans IJ, Frame B, Greenland AJ, Higgs NS, Jepson I, Jiao S, Lewnau CJ, Sillick JM, Wilson HM (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25:951-961
17. Srivastava V, Vasil V, Vasil IK (1996) Molecular characterization of the fate of transgenes in transformed wheat (Triticum aestivum L.). Theor Appl Genet 92:1031-1037
18. Tingay S, McElroy D, Kalla R, Fieg S, Wang M, Thornton S, Brettell R (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11:1369-1376
19. Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10:667-674
20. Vasil V, Srivastava V, Castillo AM, Fromm MA, Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Biotechnology 11:1553-1558
21. Weeks JT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077-1084
22. 2, backcross, doubled haploid and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genet 253:535-545