Lysozyme reactivation using immobilized molecular chaperonin GroEL

Biotechnology Techniques

Volumn 13, Issue 9, 1999, Pages 637-641

  Dong, Xiao Yan ^a   Yang, Hui ^a   Sun, Yan ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

GroEL; Immobilization; Lysozyme; Reactivation; Refolding

Indexed keywords

CHAPERONIN; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME REACTIVATION; IMMOBILIZED PROTEIN; LYSOZYME; PH; PROTEIN FOLDING;

EID: 0032873832     PISSN: 0951208X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008920515993     Document Type: Article

References (10)

1. Altamirano MM, Golbik R, Zahn R, Buckle AM, Fersht AR (1997) Refolding chromatography with immobilized mini-chaperones. Proc. Natl. Acad. Sci. USA 94: 3576-3578.
2. Fisher MT (1992) Promotion of the in vitro renaturation of dodecametric glutamine synthetase from Escherichia coli in the presence of GroEL (chaperonin-60) and ATP. Biochemistry 31: 3955-3963.
3. Guise AD, Chaudhuri JB (1998) Recovery and reuse of the molecular chaperone GroEL for in vitro protein refolding. Biotechnol. Prog. 14: 343-346.
4. Hartl F-U (1996) Molecular chaperones in cellular protein folding. Nature 381: 571-580.
5. Imoto T, Yagishita K (1971) A simple activity measurement of lysozyme. Agric. Biol. Chem. 35: 1154-1156.
6. Ishii Y, Teshima T, Kondo A, Murakami K, Sonezaki S, Ogawa H, Kato Y, Fukuda H (1997) Operation conditions of enzyme refolding by chaperonin and recycle system using ultrafiltration. Chem. Eng. J. 65: 151-157.
7. Jenkins AJ, March JB, Oliver IR, Masters M (1986) DNA fragment containing the groE genes can suppress mutations in Escherichia coli dnaA genes. Mol. Gen. Genet. 202: 446-454.
8. Mendoza JA, Rogers E, Lorimer GH, Horowitz PM (1991) Chaperonins facilitate the in vitro folding of monometric mitochondrial rhodanase. J. Biol. Chem. 266: 13044-13049.
9. Sadana A (1995) Protein refolding and inactivation during bioseparation: Bopprocessing implications. Biotechnol. Bioeng. 48: 481-489.
10. Teshima T, Kondo A, Fukuda H (1997) Reactivation of thermally inactivated enzymes by free and immobilized chaperonin GroEL/ES. Appl. Microbiol. Biotechnol. 48: 41-46.