PCR-based quantitation of Cryptosporidium parvum in municipal water samples

Journal of Microbiological Methods

Volumn 38, Issue 1-2, 1999, Pages 119-130

  Chung, E ^a   Aldom, J E ^b   Carreno, R A ^a   Chagla, A H ^b   Kostrzynska, M ^a   Lee, H ^a   Palmateer, G ^c   Trevors, J T ^a   Unger, S ^c   Xu, R ^a   De Grandis, S A ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b ONTARIO MINISTRY OF HEALTH AND LONG TERM CARE   (Canada)

^c EnviroMicrobial Services Inc   (Canada)

Author keywords

Cryptosporidium parvum; Detection; Digene SHARP Signal System(TM); Municipal water; Oocyst

Indexed keywords

AMINO ACID; BACTERIAL DNA; BACTERIAL PROTEIN; DRINKING WATER;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; DENSITOMETRY; DEVICE; GENE TARGETING; IMAGING SYSTEM; NUCLEOTIDE SEQUENCE; OOCYST; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WATER ANALYSIS;

ANIMALS; BASE SEQUENCE; CRYPTOSPORIDIUM PARVUM; DNA, PROTOZOAN; MOLECULAR SEQUENCE DATA; PARASITE EGG COUNT; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; WATER MICROBIOLOGY; WATER SUPPLY;

BACTERIA (MICROORGANISMS); CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 0032872323     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(99)00087-1     Document Type: Article

References (16)

1. Aldom J.E., Chagla A.H. Recovery of Cryptosporidium oocysts from water by a membrane filter dissolution method. Lett. Appl. Microbiol. 20:1995;186-187.
2. Bej A.K. PCR amplification of DNA recovered from the aquatic environment. Trevors J.T., Van Elsas J.D. Nucleic Acids in the Environment. 1995;179-218 Springer, New York.
3. Boivin G., Handfield J., Murray G., Toma E., Lalonde R., Lazar J.G., Bergeron M.G. Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System. J. Clin. Microbiol. 35:1997;525-526.
4. Chung E., Aldom J.E., Chagla A.H., Kostrzynska M., Lee H., Palmateer G., Trevors J.T., Unger S., De Grandis S. Detection of Cryptosporidium parvum oocysts in municipal water samples by the polymerase chain reaction. J. Microbiol. Methods. 33:1998;171-180.
5. DuPont H.L., Chappell C.L., Sterling C.R., Okhuysen P.C., Rose J.B., Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. New Eng. J. Med. 332:1995;855-859.
6. Haas C.N., Crockett C.S., Rose J.B., Gerba C.P., Fazil A.M. Assessing the risk posed by oocysts in drinking water. J. Am. Water Works Assoc. 88:1996;131-136.
7. Jenkins M., Trout J., Fayer R. A semi-quantitative method for measuring Cryptosporidium parvum infection using polymerase chain reaction. J. Microbiol. Methods. 28:1997;99-107.
8. Kostrzynska M., Sankey M., Haack E., Power C., Aldom J.E., Chagla A.H., Unger S., Palmateer G., Lee H., Trevors J.T., De Grandis S.A. Three sample preparation protocols for polymerase chain reaction based detection of Cryptosporidium parvum in environmental samples. J. Microbiol. Methods. 35:1999;65-71.
9. Laberge I., Ibrahim A., Barta J.R., Griffiths M.W. Detection of Cryptosporidium parvum in raw milk by PCR and oligonucleotide hybridization. Appl. Environ. Microbiol. 62:1996;3259-3264.
10. Lin H.J., Haywood M., Hollinger F.B. Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA. J. Clin. Microbiol. 34:1996;329-333.
11. Morgan U.M., Thompson R.C.A. PCR detection of Cryptosporidium: the way forward? Parasitol. Today. 14:1998;241-245.
12. Rose J.B., Lisle J.T., LeChevallier M. Waterborne cryptosporidiosis: incidence, outbreaks, and treatment strategies. Fayer R. Cryptosporidium and Cryptosporidiosis. 1997;93-109 CRC Press, Boca Raton.
13. Siebert P.D., Larrick J.W. Competitive PCR. Nature. 359:1992;557-558.
14. Spano F., Puri C., Ranucci L., Putignani L., Crisanti A. Cloning of the entire COWP gene of Cryptosporidium parvum and ultrastructural localization of the protein during sexual parasite development. Parasitol. 114:1997;427-437.
15. Wilkinson E.T., Cheifetz S., De Grandis S.A. Development of competitive PCR and the QPCR system 5000 as a transcription-based system. PCR Methods Appl. 4:1995;363-367.
16. Zachar V., Thomas R.A., Goustin A.S. Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples. Nucl. Acids Res. 21:1993;2017-2018.