Increases in tissue free amino acid levels in response to prolonged emersion in marine crabs: An ammonia-detoxifying process efficient in the intertidal Carcinus maenas but not in the subtidal Necora puber

Journal of Experimental Biology

Volumn 202, Issue 16, 1999, Pages 2191-2202

  Durand, Fabrice ^a   Chausson, Fabienne ^a   Regnault, Michèle ^a  

^a STATION BIOLOGIQUE   (France)

Author keywords

Air exposure; Amino acid; Carcinus maenas; Crab; Haemolymph; Hepatopancreas; Muscle; Necora puber; Nitrogen metabolism; Protein; Urate

Indexed keywords

CARCINUS MAENAS; DECAPODA (CRUSTACEA); INVERTEBRATA; NECORA; NECORA PUBER;

EID: 0032865566     PISSN: 00220949     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Ahsanullah, M. and Newell, R. C. (1977). The effects of humidity and temperature on water loss in Carcinus maenas (L.) and Portunus marmoreus (Leach). Comp. Biochem. Physiol. 56A, 593-601.
2. Boone, W. R. and Schoffeniels, E. (1979). Hemocyanin synthesis during hypo-osmotic stress in the shore crab Carcinus maenas (L.). Comp. Biochem. Physiol. 63B, 207-214.
3. Claybrook, D. L. (1983). Nitrogen metabolism. In The Biology of Crustacea, vol. 5 (ed. L. H. Mantel), pp. 163-213. New York: Academic Press.
4. Dall, W. and Smith, D. M. (1987). Changes in protein-bound and free amino acids in the muscle of the tiger prawn Penaeus esculentus during starvation. Mar. Biol. 95, 509-520.
5. deFur, P. L. and McMahon, B. R. (1984). Physiological compensation to short-term air-exposure in red rock crabs, Cancer productus Randall, from littoral and sublittoral habitats. II. Acid-base balance. Physiol. Zool. 57, 151-160.
6. De Vries, M. C. and Wolcott, D. L. (1993). Gaseous ammonia evolution is coupled to reprocessing of urine at the gills of ghost crabs. J. Exp. Zool. 267, 97-103.
7. Durand, F. and Regnault, M. (1998). Nitrogen metabolism of two portunid crabs, Carcinus maenas and Necora puber, during prolonged air exposure and subsequent recovery: a comparative study. J. Exp. Biol. 201, 2515-2528.
8. Dykens, J. A. (1991). Purineolytic capacity and origin of haemolymph urate in Carcinus maenas during hypoxia. Comp. Biochem. Physiol. 98B, 579-582.
9. Gifford, C. A. (1968). Accumulation of uric acid in the crab Cardisoma guanhumi. Am. Zool. 8, 521-528.
10. Gilles, R. (1977). Effects of osmotic stresses on the protein concentration and pattern of Eriocheir sinensis blood. Comp. Biochem. Physiol. 56A, 109-114.
11. Greenaway, P. (1991). Nitrogenous excretion in aquatic and terrestrial crustaceans. Mem. Queensland Mus. 31, 215-227.
12. Greenaway, P. and Morris, S. (1989). Adaptations to a terrestrial existence by the robber crab, Birgus lattro L. III. Nitrogenous excretion. J. Exp. Biol. 143, 333-346.
13. Greenaway, P. and Nakamura, T. (1991). Nitrogenous excretion in two terrestrial crabs (Gecarcoidea natalis and Geograpsus grayi). Physiol. Zool. 64, 767-786.
14. Harris, R. R. and Andrews, M. B. (1985). Total NPS pool and ammonia net efflux rate changes in Carcinus maenas during acclimation to low environmental salinity. Comp. Biochem. Physiol. 82A, 301-308.
15. Heath, J. R. and Barnes, H. (1970). Some changes in biochemical composition with season and during the moulting cycle of the common shore crab, Carcinus maenas (L.). J. Exp. Mar. Biol. Ecol. 5, 199-233.
16. Iwata, K. (1988). Nitrogen metabolism in the mudskipper Periophthalmus cantonensis: Changes in free amino acids and related compounds in various tissues under conditions of ammonia loading, with special reference to its high ammonia tolerance. Comp. Biochem. Physiol. 91A, 499-508.
17. 15N-ammonium sulfate: a long term experiment. Zool. Sci. 12, 175-184.
18. Johnson, I. and Uglow, R. F. (1985). Some effects of aerial exposure on the respiratory physiology and blood chemistry of Carcinus maenas (L.) and Liocarcinus puber (L.). J. Exp. Mar. Biol. Ecol. 94, 151-165.
19. Kirby, P. K. and Harbaugh, R. D. (1974). Diurnal patterns of ammonia release in marine and terrestrial isopods. Comp. Biochem. Physiol. 47A, 1313-1321.
20. Lallier, F., Boitel, F. and Truchot, J. P. (1987). The effect of ambient oxygen and temperature on haemolymph L-lactate and urate concentrations in the shore crab Carcinus maenas. Comp. Biochem. Physiol. 86A, 255-260.
21. Lallier, F. H. and Walsh, P. (1991). Activities of uricase, xanthine oxidase and xanthine dehydrogenase in the hepatopancreas of aquatic and terrestrial crabs. J. Crust. Biol. 11, 506-512.
22. Lindroth, P. and Mopper, K. (1979). High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with O-phthaldialdehyde. Analyt. Chem. 51, 1667-1674.
23. Linton, S. M. and Greenaway, P. (1997a). Intracellular purine deposits in the gecarcinid land crab Gecarcoidea natalis. J. Morph. 231, 101-110.
24. Linton, S. M. and Greenaway, P. (1997b). Urate deposits in the gecarcinid land crab Gecarcoidea natalis are synthesised de novo from excess dietary nitrogen. J. Exp. Biol. 200, 2347-2354.
25. Linton, S. M. and Greenaway, P. (1998). Enzymes of urate synthesis and catabolism in the gecarcinid land crab Gecarcoidea natalis. Exp. Biol. Online 3, 5.
26. Lowry, O., Rosenbourgh, N., Farr, A. and Randall, R. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
27. Morris, S., Bridges, C. R. and Grieshaber, M. K. (1985). A new role for uric acid: modulator of haemocyanin oxygen affinity in crustaceans. J. Exp. Zool. 235, 135-139.
28. Regnault, M. (1987). Nitrogen excretion in marine and freshwater Crustacea. Biol. Rev. 62, 1-24.
29. Regnault, M. (1992). Effect of air-exposure on nitrogen metabolism in the crab Cancer pagurus. J. Exp. Zool. 264, 372-380.
30. Regnault, M. (1994). Effect of air-exposure on ammonia excretion and ammonia content of branchial water of the crab Cancer pagurus. J. Exp. Zool. 268, 208-217.
31. Regnault, M. (1996). Air-exposure-induced increase in acetylglucosamine content of some soft tissues and haemolymph of a crab (Cancer pagurus). J. Exp. Zool. 275, 421-430.
32. Regnault, M. and Durand, F. (1998). Glycosaminoglycans in gills of an intertidal crab (Carcinus maenas): Changes in the GAG population in response to prolonged air-exposure. J. Exp. Zool. 281, 554-564.
33. Schmitt, A. S. C. and Uglow, R. F. (1997). Haemolymph constituent levels and ammonia efflux rates of Nephrops norvegicus during emersion. Mar. Biol. 127, 403-410.
34. Siebers, W. R., Lucu, C., Sperling, K.-R. and Eberlein, K. (1972). Kinetics of osmoregulation in the crab Carcinus maenas. Mar. Biol. 17, 291-303.
35. Stryer, L. (1995). Biosynthesis of amino acids and heme. In Biochemistry, fourth edition (ed. L. Stryer), pp. 713-738. New York: W. H. Freeman & Company.
36. Taylor, A. C., Spicer, J. I. and Preston, T. (1987). The relationship between osmoregulation and nitrogen metabolism in the intertidal prawn, Palaemon elegans (Rathke). Comp. Biochem. Physiol. 88A, 291-298.
37. Taylor, H. H. and Greenaway, P. (1994). The partitioning of water and solutes during evaporative water loss in three terrestrial crabs. Physiol. Zool. 67, 539-565.
38. Varley, D. G. and Greenaway, P. (1994). Nitrogenous excretion in the terrestrial carnivorous crab Geograpsus grayi: site and mechanism of excretion. J. Exp. Biol. 190, 179-193.
39. Vermeer, G. K. (1987). Effects of air-exposure on desication rate, hemolymph chemistry and escape behavior of the spiny lobster, Panulirus argus. Fishery Bull. Fish. Wildl. Serv. U.S. 85, 45-51.
40. Wieser, W. and Schweizer, G. (1970). A re-examination of the excretion of nitrogen by terrestrial isopods. J. Exp. Biol. 52, 267-274.
41. Wolcott, D. L. (1991). Nitrogen excretion is enhanced during urine recycling in two species of terrestrial crab. J. Exp. Zool. 259, 181-187.
42. Wolcott, D. L. and Wolcott, T. G. (1984). Food quality and cannibalism in the red land crab Gecarcinus lateralis. Physiol. Zool. 57, 318-324.
43. Wolcott, D. L. and Wolcott, T. G. (1987). Nitrogen limitation in the herbivorous land crab Cardisoma guanhumi. Physiol. Zool. 60, 262-268.
44. Wright, J. C., Caveney, S., O'Donnell, M. J. and Reichert, J. (1996). Increases in tissue amino acid levels in response to ammonia stress in the terrestrial isopod Porcellio scaber Latr. J. Exp. Zool. 274, 265-274.
45. Wright, J. C. and O'Donnell, M. J. (1993). Total ammonia concentration and pH of haemolymph, pleon fluid and maxillary urine in Porcellio scaber Latreille (Isopoda, Oniscidea): relationships to ambient humidity and water vapour uptake. J. Exp. Biol 176, 233-246.
46. Wright, J. C., O'Donnell, M. J. and Reichert, J. (1994). Effects of ammonia loading on Porcellio scaber: glutamine and glutamate synthesis, ammonia excretion and toxicity. J. Exp. Biol. 188, 143-157.
47. Wynants, J., Petrov, B., Nijhof, J. and Van Belle, H. (1987). Optimization of a high-performance liquid chromatographic method for determination of nucleosides and their catabolites. Application to cat and rabbit heart perfusates. J. Chromatogr. 386, 297-308.