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The twist gene: Isolation of a Drosophila zygotic gene necessary for the establishment of dorsoventral pattern
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Regional specification of muscle progenitors in Drosophila: The role of the msh homeobox gene
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Examines the origin and development of the full set of muscle progenitors and founders with a specific lacZ enhancer trap marker. The use of a msh-lacZ P-insertion allows detailed analysis of the normal expression of msh in the mesoderm as well as the muscle phenotype of msh mutants.
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Nose A, Isshiki T, Takeichi M Regional specification of muscle progenitors in Drosophila: the role of the msh homeobox gene. Development. 125:1998;215-223. Examines the origin and development of the full set of muscle progenitors and founders with a specific lacZ enhancer trap marker. The use of a msh-lacZ P-insertion allows detailed analysis of the normal expression of msh in the mesoderm as well as the muscle phenotype of msh mutants.
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The role of the NK-homeobox geneslouch (S59) in somatic muscle patterning
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in press. Shows that S59/NK1/slouch, which was the first muscle identity gene to be described, has important roles in specifying particular somatic muscles. The study identifies regulatory interactions between slouch and ladybird and presents a model explaining the observed transformations of muscle identities in slouch mutants
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Knirr S, Azpiazu N, Frasch M: The role of the NK-homeobox geneslouch (S59) in somatic muscle patterning. Development 1999, 126:in press. Shows that S59/NK1/slouch, which was the first muscle identity gene to be described, has important roles in specifying particular somatic muscles. The study identifies regulatory interactions between slouch and ladybird and presents a model explaining the observed transformations of muscle identities in slouch mutants.
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Normal expression and the effects of ectopic expression of the Drosophila muscle segment homeobox (msh) gene suggest a role in differentiation and patterning of embryonic muscles
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Describes that the homeobox gene ladybird (lb) is expressed in the progenitors/founders of muscle 8 (segment border muscle) and lateral adult precursors (LaP). Studies with deficiencies uncovering lb and ectopic lb expression indicate strongly that lb is required for specification of muscle 8/LaP. Signaling events and lineage mechanisms involved in the formation of the respective muscle founders are also examined.
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Jagla T, Bellard F, Lutz Y, Dretzen G, Bellard M, Jagla K ladybird determines cell fate decisions during diversification of Drosophila somatic muscles. Development. 125:1998;3699-3708. Describes that the homeobox gene ladybird (lb) is expressed in the progenitors/founders of muscle 8 (segment border muscle) and lateral adult precursors (LaP). Studies with deficiencies uncovering lb and ectopic lb expression indicate strongly that lb is required for specification of muscle 8/LaP. Signaling events and lineage mechanisms involved in the formation of the respective muscle founders are also examined.
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Mutant analysis demonstrates that the HLH transcription factor Collier is strictly required for the formation of muscle 3 (=DA3) from its founder cell in which collier is expressed, suggesting a role of collier in the differentiation of this particular muscle. The earliest expression of collier is observed in promuscular clusters and the paper describes how the activities of Notch, Delta, insc and numb define the collier-expressing progenitor (p3/5) and determine the founders of muscle 3 and 5
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Shows asymmetric accumulation of Inscutable and Numb proteins in muscle progenitors and demonstrates that insc acts upstream on numb to specify muscle and pericardial founder cells identities during asymmetric lineage decisions in the dorsal mesoderm.
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Extensive genetic analysis shows that Drosophila myoD is indeed required for muscle differentiation. Surprisingly, however, this requirement appears largely to be limited to the differentiation of two distinct muscles, 3 and 13, and nau mutant flies are viable. These data provide strong evidence that other muscle founders - and to some degree, also those of muscles 3 and 13 - utilize additional regulators, which act together with or instead of nau in myogenic differentiation. Note however that the study in [51], which uses RNA interference to remove nau activity, describes a significantly stronger nau phenotype. It remains to be established whether this discrepancy is due to possible artefacts associated with this newly established knock-out technique or whether there are other explanations for the differing results.
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