Mutagenic analysis of conserved arginine residues in and around the novel sulfate binding pocket of the human Theta class glutathione transferase T2-2

Protein Science

Volumn 8, Issue 10, 1999, Pages 2205-2212

  Flanagan, J U ^a   Rossjohn, J ^b   Parker, M W ^b   Board, Pg ^a   Chelvanayagam, G ^a  

^a Biology and Environment   (Australia)

^b ST VINCENT'S INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

Electrostatics; Glutathione transferase; Mechanism; Site directed mutagenesis; Sulfatase

Indexed keywords

ALANINE; ARGININE; GLUTATHIONE TRANSFERASE;

ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME STABILITY; MOLECULAR MODEL; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

EID: 0032860568     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.10.2205     Document Type: Article

References (42)

1. Armstrong RN. 1997. Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem Res Toxicol 10:2-18.
2. Asakura T, Adachi K, Schwartz E. 1978. Stabilizing effect of various organic solvents on protein. J Biol Chem 253:6423-6425.
3. Baker RT, Williamson NA, Wettenhall REH. 1996. The yeast homolog of mammalian ribosomal protein S30 is expressed from a duplicated gene without a ubiquitin-like protein fusion sequence. Evolutionary implications. J Biol Chem 271:13549-13555.
4. Bond CS, Clements PR, Ashby SJ, Collyer CA, Harrop SJ, Hopwood JJ, Guss JM. 1997. Structure of a human lysosomal sulfatase. Structure 5:277-289.
5. Burmeister WP, Cottaz S, Driguez H, Iori R, Palmieri S, Henrissat B. 1997. The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insights into the substrate recognition and active-site machinery of an S-glycosidase. Structure 5:663-675.
6. Chelvanayagam G, Wilce MCJ, Parker MW, Tan KL, Board PG. 1997. Homology model for the human GSTT2 Theta class glutathione transferase. Proteins 27:118-130.
7. Clapp JJ, Young L. 1970. Formation of mercapturic acids in rats after the administration of aralkyl esters. Biochem J 118:765-771.
8. Dirr H, Reinemer P, Huber R. 1994. X-ray crystal structure of cytosolic glutathione S-transferases. Implications for protein architecture, substrate recognition and catalytic function. Eur J Biochem 220:645-661.
9. Douglas T, Ripoll DR. 1998. Calculated electrostatic gradients in recombinant human H-chain ferritin. Protein Sci 7:1083-1091.
10. Duffel MW, Jakoby WB. 1987. Acyl group transfer - Sulphotransferases and sulphatases. In: Page MI, Williams A, eds. Enzyme mechanisms. Royal Society of Chemistry. pp 221-228.
11. Flanagan JU, Chelvanayagam G, Cassarotto M, Parker MW, Board PG. 1999. A mutagenic analysis of the role of Trp115 in the human Theta class glutathione transferase GSTT2-2. Clin Chem Enz Comms. Forthcoming.
12. Getzoff ED, Cabelli DE, Fisher CL, Parge HE, Viezzoli MS, Banci L, Hallewell RA. 1992. Faster superoxide dismutase mutants designed by enhancing electrostatic guidance. Nature 358:347-351.
13. Gillham B. 1971. The reaction of aralkyl sulphate esters with glutathione catalysed by rat liver preparations. Biochem J 121:667-672.
14. Gillham B. 1973. The mechanism of the reaction between glutathione and 1-menapthyl sulphate catalyzed by a glutathione S-transferase from rat liver. Biochem J 135:797-804.
15. Hayes JD, Pulford DJ. 1995. The glutathione S-transferase supergene family: Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. CRC Crit Rev Biochem Mol Biol 30:445-600.
16. Hiratsuka A, Kanazawa M, Nishiyama T, Okuda H, Ogura K, Watabe T. 1995. A subfamily 2 homo-dimeric glutathione S-transferase mYrs-mYrs of class Theta in mouse liver cytosol. Biochem Biophys Res Com 212:743-750.
17. Hiratsuka A, Sebata N, Kawashima K, Okuda H, Ogura K, Watabe T, Satoh K, Hatayama I, Tsuchida S. 1990. A new class of rat glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters as metabolites of carcinogenic arylmethanols. J Biol Chem 265:11973-11979.
18. Hirota-Nakaoka N, Goto Y. 1999. Alcohol-induced denaturation of beta-lactoglobulin: A close correlation to the alcohol-induced alpha-helix formation of melittin. Bioorg Med Chem 7:67-73.
19. Hussey AJ, Hayes JD. 1992. Characterization of a human class-Theta glutathione S-transferase with activity towards 1-menapthyl sulphate. Biochem J 286:929-935.
20. Jemth P, Stenberg G, Chaga G, Mannervik B. 1996. Heterologous expression, purification and characterization of rat class Theta glutathione transferase T2-2. Biochem J 316:131-136.
21. Ji X, Zhang P, Armstrong RN, Gilliland GL. 1992. The three-dimensional structure of a glutathione S-transferase from the mu gene class. Structural analysis of the binary complex of isoenzyme 3-3 and glutathione at 2.2-A resolution. Biochemistry 31:10169-10184.
22. Johnson WW, Lui S, Ji X, Gilliland GL, Armstrong RN. 1993. Tyrosine 115 participates both in chemical and physical steps of the catalytic mechanism of a glutathione S-transferase. J Biol Chem 268:11508-11511.
23. Kanaoka Y, Ago H, Inagaki E, Nanayama T, Miyano M, Kikuno R, Fujii Y, Eguchi N, Toh H, Urade Y, Hayaishi O. 1997. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90:1085-1095.
24. Lowry OH, Rosenberg NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biol Chem 193:265-275.
25. Mannervik B. Ålin P, Gulhenberg C, Jensson H, Tahir MK, Warholm M, Jornvall H. 1985. Identification of three classes of cytosolic glulathione transferase common to several mammalian species: Correlation between structural data and enzymatic properties. Proc Natl Acad Sci USA 82:7202-7206.
26. McTigue MA, Williams DR, Tainer JA. 1995. Crystal structures of a schistosomal drug and vaccine target: Glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel. J Mol Biol 246:21-27.
27. Meyer DJ, Coles B, Pemble SE, Gilmore KS, Fraser GM, Ketterer B. 1991. Theta, a new class of glutathione transferases purified from rat and man. Biochem J 274:409-414.
28. Neuefeind T, Huber R, Reinemer P, Knablein J, Prade L, Mann K, Bieseler B. 1997. Cloning, sequencing, crystallization and X-ray structure of glutathione S-transferase-III from Zea mays var. mutin: A leading enzyme in detoxification of maize herbicides. J Mol Biol 274:577-587.
29. Nishida M, Harada S, Noguchi S, Satow Y, Inoue H, Takahashi K. 1998. Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: Catalytic roles of Cys10 and His106. J Mol Biol 281:135-147.
30. Polticelli F, Bottaro G, Battistoni A, Carri MT, Djinovic-Carugo K, Bolognesi M, O'Neill P, Rotilio G, Desideri A. 1995. Modulation of the catalytic rate of Cu,Zn superoxide dismutase in single and double mutants of conserved positively and negatively charged residues. Biochemistry 34:6043-6049.
31. Reinemer P, Dirr HW, Ladenstein R, Huber R, Lo Bello M, Federici G, Parker MW. 1992. Three-dimensional structure of class pi glutathione S-transferase from human placenta in complex with S-hexylglutathione at 2.8 A resolution. J Mol Biol 227:214-226.
32. Reinemer P, Prade L, Hof P, Neuefeind T, Huber R, Zettl R, Palme K, Schell J, Koelln I, Bartunik HD, Bieseler B. 1996. Three-dimensional structure of glutathione S-transferase from Arabidopsis thaliana at 2.2 A resolution: Structural characterization of herbicide-conjugating plant glutathione S-transferases and a novel active site architecture. J Mol Biol 255:289-309.
33. Rossjohn J, Feil SC, Wilce MCJ, Sexton JL, Spithill TW, Parker MW. 1997. Crystallization, structural determination and analysis of a novel parasite vaccine candidate: Fasciola hepatica glutathione S-transferase. J Mol Biol 273:857-872.
34. Rossjohn J, McKinstry WJ, Oakley AJ, Verger D, Flanagan J, Chelvanayagam G, Tan KL, Board PG, Parker MW. 1998a. Human theta class glutathione transferase: The crystal structure reveals a sulfate-binding pocket within a buried active site. Structure 6:309-322.
35. Rossjohn J, Polekhina G, Feil SC, Allocati N, Masulli M, De Illio C, Parker MW. 1998b. A mixed disulfide bond in bacterial glutathione transferase: Functional and evolutionary implications. Structure 6:721-734.
36. Sinning I, Kleywegt GJ, Cowan SW, Reinemer P, Dirr HW, Huber R, Gilliland GL. Armstrong RN, Ji X, Board PG, et al. 1993. Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes. J Mol Biol 232:192-212.
37. Tan KL, Board PG. 1996. Purification and characterization of a recombinant human Theta-class glutathione transferase (GSTT2-2). Biochem J 315:721-732.
38. Tan KL, Chelvanayagam G, Parker MW, Board PG. 1996. Mutagenesis of the active site of the human Theta class glutathione transferase GSTT2-2: Identification of a novel function as a glutathione dependent sulphatase. Biochem J 319:315-321.
39. Thunnissen MM, Taddei N, Liguri G, Ramponi G, Nordlund P. 1997. Crystal structure of common type acylphosphatase from bovine testis. Structure 5:69-79.
40. Wade RC, Gabdoulline RR, Ludemann SK, Lounnas V. 1998. Electrostatic steering and ionic tethering in enzyme-ligand binding: Insights from simulations. Proc Natl Acad Sci USA 95:5942-5949.
41. Wilce MCJ, Board PG, Feil SC, Parker MW. 1995. Crystal structure of a theta-class glutathione transferase. EMBO J 14:2133-2143.
42. Wilce MCJ, Parker MW. 1994. Structure and function of glutathione S-transferases. Biochim Biophys Acta 1205:1-18.