Three-dimensional measurement of cerebral microvascular plasma perfusion, glial fibrillary acidic protein and microtubule associated protein-2 immunoreactivity after embolic stroke in rats: A double fluorescent labeled laser-scanning confocal microscopic study

Brain Research

Volumn 844, Issue 1-2, 1999, Pages 55-66

  Zhang, Zheng Gang ^a   Bower, Lucille ^b   Zhang, Rui Lan ^a   Chen, Song ^a   Windham, Joseph P ^b   Chopp, Michael ^a,c  

^a HENRY FORD HOSPITAL   (United States)

^b HENRY FORD HEALTH SCIENCES CENTER   (United States)

^c OAKLAND UNIVERSITY   (United States)

Author keywords

Cerebral microvascular plasma perfusion; Embolic stroke; GFAP; Laser scanning confocal microscopy; MAP2; Three dimensional image analysis; Triple fluorescent labeling

Indexed keywords

GLIAL FIBRILLARY ACIDIC PROTEIN; MICROTUBULE ASSOCIATED PROTEIN 2;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN ISCHEMIA; BRAIN MICROCIRCULATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBOLISM; FLUORESCENCE; IMAGE ANALYSIS; IMMUNOREACTIVITY; LASER MICROSCOPY; MACROGLIA; MALE; METHODOLOGY; NONHUMAN; PATHOPHYSIOLOGY; PERFUSION; PRIORITY JOURNAL; RAT; STROKE; THREE DIMENSIONAL IMAGING;

ANIMALS; ANTIBODIES; ASTROCYTES; BRAIN ISCHEMIA; CEREBRAL CORTEX; CEREBROVASCULAR CIRCULATION; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; GLIAL FIBRILLARY ACIDIC PROTEIN; IMAGE PROCESSING, COMPUTER-ASSISTED; INFARCTION, MIDDLE CEREBRAL ARTERY; INTRACRANIAL EMBOLISM; MALE; MICROCIRCULATION; MICROSCOPY, CONFOCAL; MICROTUBULE-ASSOCIATED PROTEINS; NEURONS; PLASMA; RATS; RATS, WISTAR;

EID: 0032859404     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(99)01886-7     Document Type: Article

References (38)

1. Abbott N.J., Revest P.A., Romero I.A. Astrocyte-endothelial interaction: physiology and pathology. Neuropathol. Appl. Neurobiol. 18:1992;424-433.
2. Ames A. III, Wright R.L., Kowada M., Thurston J.M., Majno G. Cerebral ischemia: II. The no-reflow phenomenon. Am. J. Pathol. 52:1968;437-453.
3. Chen H., Chopp M., Schultz L., Bodzin G., Garcia J.H. Sequential neuronal and astrocytic changes after transient middle cerebral artery occlusion in the rat. J. Neurol. Sci. 118:1993;109-116.
4. Dawson D.A., Hallenbeck J.M. Acute focal ischemia-induced alterations in MAP2 immunostaining: description of temporal changes and utilization as a marker for volumetric assessment of acute brain injury. J. Cereb. Blood Flow Metab. 16:1996;170-174.
5. Dawson D.A., Ruetzler C.A., Hallenbeck J.M. Temporal impairment of microcirculatory perfusion following focal cerebral ischemia in the spontaneously hypertensive rat. Brain Res. 749:1997;200-208.
6. del Zoppo G.J. Microvascular changes during cerebral ischemia and reperfusion. Cerebrovasc. Brain Metab. Rev. 6:1994;47-96.
7. Eng L.F. Glial fibrillary acidic protein (GFAP): the major protein of glial intermediate filaments in differentiated astrocytes. J. Neuroimmunol. 8:1985;203-214.
8. Eng L.F., DeArmond S.J. Glial fibrillary acidic (GFAP) protein immunocytochemistry in development and neuropathology. Prog. Clin. Biol. Res. 59A:1981;65-79.
9. Eng L.F., Vanderhaeghen J.J., Bignami A., Gerstl B. An acidic protein isolated from fibrous astrocytes. Brain Res. 28:1971;351-354.
10. Fischer E.G., Ames A. III, Hedley-Whyte E.T., O'Gorman S. Reassessment of cerebral capillary changes in acute global ischemia and their relationship to the no-reflow phenomenon. Stroke. 8:1977;36-39.
11. Garcia J.H., Liu K.F., Yoshida Y., Chen S., Lian J. Brain microvessels: factors altering their patency after the occlusion of a middle cerebral artery (Wistar rat). Am. J. Pathol. 145:1994;728-740.
12. Garcia J.H., Ye Z.R., Liu K.F., Gutierrez J.A. Astrocytes disintegration precedes the appearance of dead neurons in rats with focal brain ischemia. J. Cereb. Blood Flow Metab. 17:1997;S105.
13. Garcia J.H., Yoshida Y., Chen H., Li Y., Zhang Z.G., Lian J., Chen S., Chopp M. Progression from ischemic injury to infarct following middle cerebral artery occlusion in the rat. Am. J. Pathol. 142:1993;623-635.
14. K.A. Hossmann, Hemodynamics of post-ischemic reperfusion of the brain, in: P.R. Weistein, A.L. Faden (Eds.), Protection of the Brain from Ischemia, William and Wilkins, Baltimore, 1990, pp. 21-36.
15. Ito U., Hanyu S., Hakamata Y., Nakamura M., Arima K. Ultrastructure of astrocytes associated with selective neuronal death of cerebral cortex after repeated ischemia. Acta Neurochir., Suppl. (Wien). 70:1997;46-49.
16. Johnson G.V., Jope R.S. The role of microtubule-associated protein 2 (MAP-2) in neuronal growth, plasticity, and degeneration. J. Neurosci. Res. 33:1992;505-512.
17. Kempski O., Staub F., Jansen M., Schodel F., Baethmann A. Glial swelling during extracellular acidosis in vitro. Stroke. 19:1988;385-392.
18. Kempski O.S., Volk C. Neuron-glial interaction during injury and edema of the CNS. Acta Neurochir., Suppl. (Wien). 60:1994;7-11.
19. Kempski O., von Rosen S., Weigt H., Staub F., Peters J., Baethmann A. Glial ion transport and volume control. Ann. NY Acad. Sci. 633:1991;306-317.
20. Li Y., Chopp M., Zhang Z.G., Zhang R.L. Expression of glial fibrillary acidic protein in areas of focal cerebral ischemia accompanies neuronal expression of 72-kDa heat shock protein. J. Neurol. Sci. 128:1995;134-142.
21. Little J.R., Kerr F.W., Sundt T.M. Jr. Microcirculatory obstruction in focal cerebral ischemia: an electron microscopic investigation in monkeys. Stroke. 7:1976;25-30.
22. Matesic D.F., Lin R.C. Microtubule-associated protein 2 as an early indicator of ischemia-induced neurodegeneration in the gerbil forebrain. J. Neurochem. 63:1994;1012-1020.
23. Naganuma Y. Changes of the cerebral microvascular structure and endothelium during the course of permanent ischemia. Keio J. Med. 39:1990;26-31.
24. Norenberg M.D. Astrocyte responses to CNS injury. J. Neuropathol. Exp. Neurol. 53:1994;213-220.
25. Ono S., Date I., Nakajima M., Onoda K., Ogihara K., Shiota T., Asari S., Ninomiya Y., Yabuno N., Ohmoto T. Three-dimensional analysis of vasospastic major cerebral arteries in rats with the corrosion cast technique. Stroke. 28:1997;1631-1637.
26. G. Paxinos, C. Watson, The Rat Brain in Stereotaxic Coordinates, 2nd edn., Academic Press, New York, 1986.
27. Petito C.K., Babiak T. Early proliferative changes in astrocytes in postischemic noninfarcted rat brain. Ann. Neurol. 11:1982;510-518.
28. Petito C.K., Chung M., Halaby I.A., Cooper A.J. Influence of the neuronal environment on the pattern of reactive astrocytosis following cerebral ischemia. Prog. Brain Res. 94:1992;381-387.
29. Petito C.K., Halaby I.A. Relationship between ischemia and ischemic neuronal necrosis to astrocyte expression of glial fibrillary acidic protein. Int. J. Dev. Neurosci. 11:1993;239-247.
30. Petito C.K., Morgello S., Felix J.C., Lesser M.L. The two patterns of reactive astrocytosis in postischemic rat brain. J. Cereb. Blood Flow Metab. 10:1990;850-859.
31. Siushansian R., Tao L., Dixon S.J., Wilson J.X. Cerebral astrocytes transport ascorbic acid and dehydroascorbic acid through distinct mechanisms regulated by cyclic AMP. J. Neurochem. 68:1997;2378-2385.
32. Soltanian-Zadeh H., Windham J.P. Mathematical basis of eigenimage filtering. Magn. Reson. Med. 31:1994;465-467.
33. Walz W., Klimaszewski A., Paterson I.A. Glial swelling in ischemia: a hypothesis. Dev. Neurosci. 15:1993;216-225.
34. Windham J.P., Abd-Allah M.A., Reimann D.A., Froelich J.W., Haggar A.M. Eigenimage filtering in MR imaging. J. Comput. Assist Tomogr. 12:1988;1-9.
35. Yamashita K., Vogel P., Fritze K., Back T., Hossmann K.A., Wiessner C. Monitoring the temporal and spatial activation pattern of astrocytes in focal cerebral ischemia using in situ hybridization to GFAP mRNA: comparison with sgp-2 and hsp70 mRNA and the effect of glutamate receptor antagonists. Brain Res. 735:1996;285-297.
36. Zhang R.L., Chopp M., Zhang Z.G., Jiang Q., Ewing J.R. A rat model of focal embolic cerebral ischemia. Brain Res. 766:1997;83-92.
37. Zhang Z.G., Davies K., Prostak J., Fenstermacher J., Chopp M. Quantitation of microvascular plasma reperfusion and neuronal microtubulin-associated protein in ischemic mouse brain by laser-scanning confocal microscopy. J. Cereb. Blood Flow Metab. 19:1999;68-78.
38. Zilles K., Hajos F., Kalman M., Schleicher A. Mapping of glial fibrillary acidic protein-immunoreactivity in the rat forebrain and mesencephalon by computerized image analysis. J. Comp. Neurol. 308:1991;340-355.