Ligase detection reaction for identification of low abundance mutations

Clinical Biochemistry

Volumn 32, Issue 4, 1999, Pages 287-290

  Khanna, Marilyn ^a   Cao, Weiguo ^a   Zirvi, Monib ^a   Paty, Philip ^b   Barany, Francis ^a  

^a Hearst Microbiology Research Center   (United States)

^b Dept Surg Mem Sloan Kettering C ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

ARTICLE; CANCER CELL; DNA TEMPLATE; ENZYME SPECIFICITY; GENE MUTATION; GENE SEQUENCE; PRIORITY JOURNAL;

BASE PAIR MISMATCH; CELLS, CULTURED; DNA LIGASES; DNA MUTATIONAL ANALYSIS; GENES, RAS; HUMANS; POLYMERASE CHAIN REACTION; TEMPLATES, GENETIC; THERMUS THERMOPHILUS;

EID: 0032857635     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(99)00020-X     Document Type: Article

References (6)

1. Barany F. The ligase chain reaction (LCR) in a PCR world. PCR Methods and Applications. 1:1991;5-16.
2. Barany F. Genetic disease detection and DNA amplification using cloned thermostable ligase. Proc Natl Acad Sci U S A. 88:1991;189-193.
3. Luo J., Bergstrom D., Barany F. Improving the specificity of Thermus thermophilus DNA ligase. Nucleic Acids Res. 24:1996;3071-3078.
4. Bos J.L., Fearon E.R., Hamilton S.R., et al. Prevalence of ras gene mutations in human colorectal cancers. Nature. 327:1987;293-297.
5. Forrester K., Almoguera C., Han K., Grizzle W.E., Perucho M. Detection of high incidence of K-ras oncogenes during human colon tumorigenesis. Nature. 327:1987;298-303.
6. Fearon E.R., Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 61:1990;759-767.