The relative semi-quantification of mRNA expression as a useful toxicological endpoint for the identification of embryotoxic/teratogenic substances

Toxicology in Vitro

Volumn 13, Issue 4-5, 1999, Pages 619-623

  Bigot, K ^a   De Lange, J ^a   Archer, G ^a   Clothier, R ^b   Bremer, S ^a  

^a JOINT RESEARCH CENTRE   (Italy)

^b NONE

Author keywords

Embryonic stem cells; Embryotoxicity; PCR analysis; Teratogenicity

Indexed keywords

CAFFEINE; CYTARABINE; FLUOROURACIL; ISONIAZID; MESSENGER RNA; MYOSIN HEAVY CHAIN; PENICILLIN G; PHENYTOIN; RETINOIC ACID; SACCHARIN; TERATOGENIC AGENT;

ANIMAL CELL; CONFERENCE PAPER; CONTROLLED STUDY; CYTOTOXICITY; EMBRYO CELL; EMBRYOTOXICITY; MOUSE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL; TERATOGENICITY;

EID: 0032856081     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(99)00027-2     Document Type: Conference Paper

References (21)

1. Arnold D.L. Toxicology of saccharin. Fundamental and Applied Toxicology. 4:1984;674-685.
2. Boucher D., Delost P. Developpement post-natal des descendants issus de meres traitees par la penicilline au cours de la gestation chez la souris. C.R. Societe de Biologie (Paris). 158:1964;528-532.
3. Dagg C.P. Sensitive stages for the production of developmental abnormalities in mice with 5-fluorouracil. American Journal of Anatomy. 106:1960;89-96.
4. Doetschman T.C., Eistetter H., Katz M., Schmidt W., Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. Journal of Embryology and Experimental Morphology. 87:1985;27-45.
5. Flint O.P. In vitro tests for teratogens: desirable endpoints, test batteries and current status of the micromass teratogen test. Reproductive Toxicology. 7:1993;1-103.
6. Geelen J.A.G. Hypervitaminosis A induced teratogenesis. CRC Critical Reviews in Toxicology. 6:1979;351-375.
7. Kalter H. Nonteratogenicity of isoniazid in mice. Teratology. 5:1972;259.
8. Keller G., Kennedy M., Papayannopoulou T., Wiles M.V. Hematopoietic commitment during embryonic stem cell differentiation in culture. Molecular Cellular Biology. 13:1993;473-486.
9. Lammer E.J., Chen D.T., Hoar R.M., Agnish N.D., Benke P.J., Braun J.T., Femhoff P.M., Grix A.W. Jr, Lott I.T., Richard J.M., Sun S.C. Retinoic acid embryopathy. New England Journal of Medicine. 313:1985;837-841.
10. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 65:1983;55-63.
11. Newall D.R., Beedles K.E. The stem cell test-A novel in vitro assay for teratogenic potential. Toxicology in Vitro. 8:1994;697-701.
12. Ortega A., Puig M., Domingo J.L. Maternal and developmental toxicity of low doses of cytosine arabinoside in mice. Teratology. 44:1991;379-384.
13. Piersma A.H., Haakmat A.S., Hagenaars A.M. In vitro assays for the developmental toxicity of xenobiotic compounds using differentiating embryonic carcinoma cells in culture. Toxicology in Vitro. 7:1993;615-621.
14. Robbins J., Gulick J., Sanchez A., Howles P., Doetschman T. Mouse embryonic stem cells express the cardiac myosin heavy chain genes during development in vitro. Journal of Biological Chemistry. 265:1990;11905-11909.
15. Rudnicki M. A. and McBurney M. W. (1987) Cell culture methods and induction of differentiation of embryonal carcinoma cell lines. In Teratocarcinoma and Embryonic Stem Cells: A Practical Approach. Edited by E. J. Robertson, pp. 19-49. IRL Press, Washington, DC.
16. Seller M.J., Perkins K.J. Effect of Hydroxyurea on neural tube defects in the curly-tail mouse. Journal of Craniofacial Genetics and Developmental Biology. 3:1983;11-17.
17. Smith M.K., Kimmel G.L., Kochhar D.M., Shepard T.H., Spielberg S.P., Wilson J.G. A selection of candidate compounds for in vitro teratogenesis test validation. Teratogenesis, Carcinogenesis, and Mutagenesis. 3:1983;461-480.
18. Tanaka R. LD50 of saccharin or cyclamate for mice embryos in the 7th day of pregnancy (fetal median lethal dose: FLD50). Journal of the Iwate Medicine Association. 16:1964;330-337.
19. Watkinson W.P., Millicovsky G. Effect of phenytoin on maternal heart rate in A/J mice: possible role in teratogenesis. Teratology. 28:1983;1-8.
20. Williams R.C., Hilton D.J., Peace S., Willson T.A., Stewart C.C., Gearing D.P., Wagner E.F., Metcalf D., Nicola N.A., Gough N.M. Myeloid leukaemia inhibitory factor maintains the development potential of embryonic stem cells. Nature. 336:1988;684-687.
21. Wobus A.M., Grosse R., Schoeneich J. Specific effects of nerve growth factor on the differentiation pattern of mouse embryonic stem cells in vitro. Biomedica et Biochimica Acta. 47:1988;965-973.