Selective determination of fish aspartate aminotransferase isoenzymes by their differential sensitivity to proteases

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 124, Issue 2, 1999, Pages 209-214

  Petrovic S ^a   Semencic L ^a   Ozretic B ^a   Krajnovic Ozretic M ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

Aspartate aminotransferase (AAT); Chymotrypsin; Cytosolic AAT; Enzyme inactivation; Fish plasma; Grey mullet; Isoenzymes; Mitochondrial AAT; Proteolytic enzymes

Indexed keywords

ASPARTATE AMINOTRANSFERASE; CHYMOTRYPSIN; ISOENZYME;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; ENZYME INACTIVATION; FISH; GEL ELECTROPHORESIS; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION;

MUGIL AURATUS;

EID: 0032855090     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(99)00119-4     Document Type: Article

References (21)

1. Beyer J, Sandvik M, Hylland K, Fjedl E, Egaas E, Aas E, Skåre JU, Goksøyr A. Contaminant accumulation and biomarker responses in flounder (Platichthys flesus L.) and atlantic cod (Gadus morhua L.) Exposed by caging to polluted sediments in Sørfjorden, Norway. Aquat Toxicol 1996;36:75-98.
2. Beyer J, Sandvik M, Skåre JU, Egaas E, Hylland K, Waagbø R, Goksøyr A. Time- and dose-dependent biomarker response in flounder (Platichthys flesus L.) exposed to benzo(a)pyrene, 2,3,3′,4,4′, 5-hexachlorobiphenyl (PCB-156) and cadmium. Biomarkers 1997;2:35-44.
3. Casillas E, Myers M, Ames WE. Relationship of serum chemistry values to the liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride. Aquat Toxicol 1983;3:61-78.
4. Casillas E, Ames WE. Hepatotoxic effects of CC14 on English sole (Parophrys vetulus): possible indicators of liver dysfunction. Comp Biochem Physiol 1986;84C:397-400.
5. Folmar LC. Effects of chemical contaminants on blood chemistry of teleost fish: a bibliography and synopsis of selected effects. Environ Toxicol Chem 1993;12:337-75.
6. Gill TS, Tewari H, Pande J. Use of the fish enzyme system in monitoring water quality: effects of mercury on tissue enzymes. Comp Biochem Physiol 1990;97C:287-92.
7. Gill TS, Tewari H, Pande J. In vivo and in vitro effects of cadmium on selected enzymes in different organs of the fish Barbus conchonius Ham (Rosy barb). Comp Biochem Physiol 1991;100C:501-5.
8. Iriarte A, Hubert E, Kraft K, Martinez-Carrion M. Selective tryptic cleavage of native cytoplasmic aspartate transaminase holoenzyme. J Biol Chem 1984;259:723-8.
9. Karmen A. A note on the spectrophotometric assay of glutamic-oxaloacetic transaminase in human blood serum. J Clin Invest 1955;34:131-3.
10. Krajnović-Ozretić M, Ozretić B. Estimation of the enzymes LDH, GOT and GPT in plasma of grey mullet Mugil auratus and their significance in liver intoxication. Dis Aquat Org 1987;3:187-93.
11. Krajnović-Ozretić M, Ozretić B. Specific alteration of transaminases in marine organisms under pollution impact. In: Vernberg WB, Calabrese A, Thurberg FP, Vernberg FJ, editors. Pollution Physiology of Estuarine Organisms. Columbia: University of South Carolina Press, 1987:207-30.
12. Ojala K, Konttinen A. A sensitive and simple method for the determination of aspartate aminotransferase isoenzymes. In: Enzymes in health and disease. Inaug Sci Meet Int Soc Clin Enzymol. Basel: Karger, 1978:118-22.
13. Panteghini M. Aspartate aminotransferase isoenzymes. Clin Biochem 1990;23:311-9.
14. Panteghini M, Bonora R, Pagani F. Automated measurement of mitochondrial aspartate aminotransferase by selective proteolysis with proteinase K. Clin Chem 1993;39:2199-200.
15. Petrović S, Krajnović-Ozretić M, Ozretić B. Isolation and properties of mitochondrial aspartate aminotransferase from red muscle of grey mullet, Mugil auratus Risso. Biochem Biophys Acta 1994;1207:201-7.
16. Petrović S, Ozretić B, Krajnović-Ozretić M. Cytosolic aspartate aminotransferase from the grey mullet (Mugil auratus Risso) red muscle: isolation and properties. Int J Biochem Cell Biol 1996;28:873-81.
17. Racicot JG, Gaudet M, Leray C. Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: Study of CC14 toxicity and a case of Aeromonas infection. J Fish Biol 1975;7:825-35.
18. Rej R. Aspartate aminotransferase activity and isoenzymes proportions in human liver tissues. Clin Chem 1978;24:1971-9.
19. Sandmeier E, Christen P. Mitochondrial aspartate aminotransferase 27/32-410-partially active enzyme derivative produced by limited proteolytic cleavage of native enzyme. J Biol Chem 1984;255:10284-9.
20. Teranishi H, Morino Y, Nishino T, Murao S, Yagi T, Kagamiyama H, Harada N. Determination of human aspartate aminotransferase isoenzymes by their differential sensitivity to proteases. Clin Biochem 1988;21:347-52.
21. Watazu Y, Okabe H, Sogiuchi H, Uji Y, Shirahase Y, Kaneda N. Proteolytic measurement of mitochondrial aspartate aminotransferase in human serum. Clin Biochem 1990;23:127-30.