A 140-kDa glycopeptide from the sperm ligand of the vitelline coat of the freshwater bivalve Unio elongatulus, only contains O-linked oligosaccharide chains and mediates sperm-egg interaction

Molecular Reproduction and Development

Volumn 54, Issue 2, 1999, Pages 203-207

  Capone, Antonietta ^a   Rosati, Floriana ^a   Focarelli, Riccardo ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

Fertilization; Glycoconjugates; Lectins; O linked oligosaccharides; Sperm

Indexed keywords

GLYCOCONJUGATE; GLYCOPEPTIDE; LECTIN; LIGAND; OLIGOSACCHARIDE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FERTILIZATION; MOLLUSC; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; SPERM;

ANIMALIA; ARACHIS HYPOGAEA; BIVALVIA; CANAVALIA ENSIFORMIS; INVERTEBRATA; LOTUS TETRAGONOLOBUS; MOLLUSCA; TETRAGONOLOBUS; UNIO; UNIO ELONGATULUS;

EID: 0032854972     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1098-2795(199910)54:2<203::aid-mrd13>3.0.co;2-%23     Document Type: Article

References (25)

1. Aitken A, Geisow MJ, Findlay JBC, Holmes C, Yarwood A. 1989. Peptide preparation and characterization. In: Findlay JBC, Geisow MJ, editors. Protein sequencing. Oxford: Oxford University Press, p 43-68.
2. Dhume ST, Lennarz WJ. 1995. The involvement of O-linked oligosaccharide chains of the sea urchin egg receptor for sperm in fertilization. Glycobiology 5:11-17.
3. Florman HM, Wassarman PM. 1985. O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity. Cell 41:313-324.
4. Focarelli R, Rosati F. 1993. Vitelline coat of Unio elongatulus egg: I. Isolation and biochemical characterization. Mol Reprod Dev 35: 4451.
5. Focarelli R, Rosati F. 1995. The 220 kD vitelline coat glycoprotein mediates sperm binding in the polarized egg of Unio elongatulus through O-linked oligosaccharides. Dev Biol 171:606-614.
6. Focarelli R, Renieri T, Rosati F. 1988. Polarized site of sperm entrance in the egg of a freshwater bivalve, Unio elongatulus. Dev Biol 127: 443-451.
7. Focarelli R, Santucci A, Lampariello R, Rosati F. 1995a. Vitelline coat of Unio elongatulus: II. Biochemical properties of the 220- and 180-kD components. Mol Reprod Dev 40:116-121.
8. Focarelli R, Leotta F, Lampariello R, Rosati F. 1995b. Vitelline coat of Unio elongatulus: III. Analysis of the 220- and 180-kD components by means of lectins. Mol Reprod Dev 40:205-210.
9. Ishihara K, Oguri K, Taniguchi H. 1973. Isolation and characterization of fucose sulfate from jelly coat glycoprotein of sea urchin egg. Biochim Biophys Acta 320:628-634.
10. Keller SH, Vacquier VD. 1994. N-linked oligosaccharides of sea urchin egg jelly induce the sperm acrosome reaction. Dev Growth Diff 36:551-556.
11. Knudsen KA. 1985. Proteins transferred to nitrocellulose for use as immunogens. Anal Biochem 147:285-288.
12. Koyota S, Wimalasiri KM, Hoshi M. 1997. Structure of the main saccharide chain in the acrosome reaction-inducing substance of the starfish, Asterias amurensis. J Biol Chem 272:10372-10376.
13. Kyozuka K, Osanai K. 1985. Sperm entry in oyster (Crassostrea gigas). Bull Mar Biol Stn Asamushi, Tuhoku Univ 18:25-34.
14. Litscher ES, Wassarman PM. 1996. Characterization of a mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome-reaction inducing activity in vitro. Biochemistry 35:3980-3985.
15. Robertson EF, Dannelly HK, Malloy PJ, Reeves HC. 1987. Rapid isolectric focusing in a vertical polyacrylamide minigel system. Anal Biochem 167:290-294.
16. Rosati F. 1985. Sperm-egg interactions in ascidians. In: Metz CB, Monroy A, editors. Biology of fertilization, vol. 2. New York: Academic Press, p 361-384.
17. Rosati F. 1995. Sperm-egg interactions during fertilization in invertebrates. Boll Zool 62:323-334.
18. Sato M, Osanai K. 1990. Sperm attachment and acrosome reaction on the egg surface of the polychaete Tyllorrhynchus heterochaetus. Biol Bull 178:101-110.
19. Schagger H, von Jagow G. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368-379.
20. Sinowatz F, Topfer-Petersen E, Calvete JJ. 1997. Glycobiology of fertilization. In: Gabius HJ, Gabius S, editors. Glycosciences: status and perspectives. Weinheim, Chapman & Hall, p 595-610.
21. Talbot P, Poolsanguan B, Al-Hajj H, Poolsanguan W. 1991. Gamete interactions during in vitro fertilization of lobster (Homarus americanus) oocytes. J Struct Biol 106:125-134.
22. Towbin H, Staehelin T. Gordon S. 1979. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 79:1908-1911.
23. Van den Steen P, Rudd PM, Dwek RA, Opdenakker G. 1998. Concepts and principles of O-glycosylation. Crit Rev Biochem Mol Biol 33:151-208.
24. Varki A. 1997. Selectin ligands: will the real ones please stand up? J Clin Invest 100:S31-S35.
25. Yurewicz EC, Pack BA, Sacco GA. 1992. Porcine oocyte zona pellucida Mr 55,000 glycoproteins: identification of O-glycosylated domain. Mol Reprod Dev 33:182-188.