Identification of amino acid residues of influenza virus nucleoprotein essential for RNA binding

Journal of Virology

Volumn 73, Issue 9, 1999, Pages 7357-7367

  Elton, Debra ^a   Medcalf, Liz ^a   Bishop, Konrad ^a   Harrison, Deborah ^a   Digard, Paul ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGININE; PHENYLALANINE; RNA POLYMERASE; TRYPTOPHAN; VIRUS NUCLEOPROTEIN; VIRUS RNA;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; FLUORESCENCE ANALYSIS; GENE EXPRESSION; MOLECULAR INTERACTION; MUTAGENESIS; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN RNA BINDING;

EID: 0032854172     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.9.7357-7367.1999     Document Type: Article

References (50)

1. Albo, C., A. Valencia, and A. Portela. 1995. Identification of an RNA binding region within the N-terminal third of the influenza A virus nucleoprotein. J. Virol. 69:3799-3806.
2. Bandziulis, R. J., M. S. Swanson, and G. Dreyfuss. 1989. RNA-binding proteins as developmental regulators. Genes Dev. 3:431-442.
3. Baudin, F., C. Bach, S. Cusak, and R. W. H. Ruigrok. 1994. Structure of influenza RNP. I. Influenza virus nucleoprotein melts secondary structure in panhandle RNA and exposes the bases to solvent. EMBO J. 13:3158-3165.
4. Beaton, A. R., and R. M. Krug. 1986. Transcription antitermination during influenza viral template RNA synthesis requires the nucleocapsid proteins and the absence of a 5′ capped end. Proc. Natl. Acad. Sci. USA 83:6282-6286.
5. Biswas, S. K., P. L. Boutz, and D. P. Nayak. 1998. Influenza virus nucleoprotein interacts with influenza virus polymerase proteins. J. Virol. 72:5493-5501.
6. Blok, V., C. Cianci, K. W. Tibbles, S. C. Inglis, M. Krystal, and P. Digard. 1996. Inhibition of the influenza virus RNA-dependent RNA polymerase by antisera directed against the carboxy-terminal region of the PB2 subunit. J. Gen. Virol. 77:1025-1033.
7. Bochkarev, A., R. A. Pfuetzner, A. M. Edwards and L. Frappier. 1997. Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Nature 385:176-181.
8. Brun, F., J.-J. Toulme, and C. Helene. 1975. Interactions of aromatic residues of proteins with nucleic acids. Fluorescence studies of the binding of oligopeptides containing tryptophan and tyrosine residues to polynucleotides. Biochemistry 14:558-563.
9. Burd, C. G., and G. Dreyfuss. 1994. Conserved structures and diversity of functions of RNA-binding proteins. Science 265:615-621.
10. asp recognition by its cognate class II aminoacyl-tRNA synthetase. Nature 362:181-184.
11. Choppin, P. W., and H. W. Compans. 1975. The structure of influenza virus, p. 15-51. In E. D. Kilbourne (ed.), The influenza viruses and influenza. Academic Press, Inc., New York, N.Y.
12. Digard, P., V. C. Blok, and S. C. Inglis. 1989. Complex formation between influenza virus polymerase proteins expressed in Xenopus oocytes. Virology 171:162-169.
13. Digard, P., D. Elton, K. Bishop, E. Medcalf, A. Weeds, and B. Pope. 1999. Modulation of nuclear localization of influenza virus nucleoprotein through interaction with actin filaments. J. Virol. 73:2222-2231.
14. Duesberg, P. 1969. Distinct subunits of the ribonucleoprotein of influenza virus. J. Mol. Biol. 42:485-499.
15. Duplay, P., H. Bedouelle, A. Fowler, I. Zabin, W. Saurin, and M. Hofnung. 1984. Sequences of the malE gene and its product, the maltose-binding protein of Escherichia coli K12. J. Biol. Chem. 259:10606-10613.
16. Elton, D., E. Medcalf, K. Bishop, and P. Digard. Oligomerisation of influenza A virus nucleoprotein: identification of positive and negative sequence elements. Virology, in press.
17. Fields, R. 1972. The rapid determination of amino groups with TNBS. Methods Enzymol. 25:464-468.
18. Fuerst, T. R., P. L. Earl, and B. Moss. 1987. Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes. Mol. Cell. Biol. 7: 2538-2544.
19. Goldstein, E. A., and M. W. Pons. 1970. The effect of polyvinylsulphate on the ribonucleoprotein of influenza virus. Virology 41:382-384.
20. Gorlach, M., M. Wittekind, R. A. Beckman, L. Mueller, and G. Dreyfuss. 1992. Interaction of the RNA-binding domain of the hnRNP C proteins with RNA. EMBO J. 11:3289-3295.
21. Guan, C., P. Li, P. D. Riggs, and H. Inouye. 1987. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67:21-30.
22. Habeeb, A. F. S. A. 1966. Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. Anal. Biochem. 14:328-336.
23. Honda, A., K. Ueda, K. Nagata, and A. Ishihama. 1988. RNA polymerase of influenza virus: role of NP on RNA chain elongation. J. Biochem. 104: 1021-1026.
24. Huang, T.-S., P. Palese, and M. Krystal. 1990. Determination of influenza virus proteins required for genome replication. J. Virol. 64:5669-5673.
25. Jennings, P. A., J. T. Finch, G. Winter, and J. S. Robertson. 1983. Does the higher order structure of the influenza virus ribonucleoprotein guide the sequence rearrangements in influenza viral RNA? Cell 34:619-627.
26. Jessen, T. H., C. Oubridge, C. H. Teo, C. Pritchard, and K. Nagai. 1991. Identification of molecular contacts between the U1 A small nuclear ribonucleoprotein and U1 RNA. EMBO J. 10:3447-3456.
27. Kingsbury, D. W., I. M. Jones, and K. G. Murti. 1987. Assembly of influenza ribonucleoprotein in vitro using recombinant nucleoprotein. Virology 156: 396-403.
28. Klump, K., R. W. H. Ruigrok, and F. Baudin. 1997. Roles of the influenza virus polymerase and nucleoprotein in forming a functional RNP structure. EMBO J. 16:1248-1257.
29. Kobayashi, M., T. Toyoda, D. M. Adyshev, Y. Azuma, and A. Ishihama. 1994. Molecular dissection of influenza virus nucleoprotein: deletion mapping of the RNA binding domain. J. Virol. 68:8433-8436.
30. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
31. Lamb, R. A., and R. M. Krug. 1996. Orthomyxoviridae: the viruses and their replication, p. 1353-1396. In B. N. Fields, D. M. Knipe, P. M. Howley, et al. (ed.), Fields virology, 3rd ed. Lippincott-Raven, Philadelphia, Pa.
32. Landon, M. 1977. Cleavage at aspartyl-prolyl bonds. Methods Enzymol. 47: 145-149.
33. Li, R., P. Palese, and M. Krystal. 1989. Complementation and analysis of an NP mutant of influenza virus. Virus Res. 12:97-112.
34. Mandler, J., and C. Scholtissek. 1989. Localisation of the temperature-sensitive defect in the nucleoprotein of an influenza A/FPV/Rostock/34 virus. Virus Res. 12:113.
35. Medcalf, L., E. Poole, D. Elton, and P. Digard. 1999. Temperature-sensitive lesions in two influenza A viruses defective for replicative transcription disrupt RNA binding by the nucleoprotein. J. Virol. 73:7349-7356.
36. O'Neill, R. E., and P. Palese. 1995. NPI-1, the human homologue of SRP-1, interacts with influenza virus nucleoprotein. Virology 206:116-125.
37. O'Neill, R. E., R. Jaskunas, G. Blobel, P. Palese, and J. Moroianu. 1995. Nuclear import of influenza virus RNA can be mediated by viral nucleoprotein and transport factors required for protein import. J. Biol. Chem. 270: 22701-22704.
38. Oubridge, C., N. Ito, P. R. Evans, C.-H. Teo, and K. Nagai. 1994. Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin. Nature 372:432-438.
39. Pons, M. W., I. T. Schulze, G. K. Hirst, and R. Hauser. 1969. Isolation and characterization of the ribonucleoprotein of influenza virus. Virology 39: 250-259.
40. Predki, P. F., L. M. Nayak, M. B. C. Gottlieb, and L. Regan. 1995. Dissecting RNA-protein interactions: RNA-RNA recognition by Rop. Cell 80:41-50.
41. Ruigrok, R. W. H., and F. Baudin. 1995. Structure of influenza virus ribonucleoprotein particles. II. Purified RNA-free influenza virus ribonucleoprotein forms structures that are indistinguishable from the intact influenza virus ribonucleoprotein particles. J. Gen. Virol. 76:1009-1014.
42. Schmid, F. X. 1989. Spectral methods of characterising protein conformation and conformational changes, p 251-285. In T. E. Creighton (ed.), Protein structure. A practical approach. IRL Press, Oxford, United Kingdom.
43. Scholtissek, C., and H. Becht. 1971. Binding of ribonucleic acids to the RNP-antigen protein of influenza viruses. J. Gen. Virol. 10:11-16.
44. Shamoo, Y., A. M. Friedman, M. R. Parsons, W. H. Konigsberg, and T. A. Steitz. 1995. Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA. Nature 376:362-366.
45. Shapiro, G. I., and R. M. Krug. 1988. Influenza virus RNA replication in vitro: synthesis of viral template RNAs and virion RNAs in the absence of an added primer. J. Virol. 62:2285-2290.
46. Smith, G. L., J. Z. Levin, P. Palese, and B. Moss. 1987. Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses. Virology 160:336-345.
47. Wang, P., P. Palese, and R. E. O'Neil. 1997. The NPI-1/NPI-3 (karyopherin α) binding site on the influenza A virus nucleoprotein NP is a nonconventional nuclear localization signal. J. Virol. 71:1850-1856.
48. Winter, G., and S. Fields. 1981. The structure of the gene encoding the nucleoprotein of human influenza virus A/PR/8/34. Virology 114:423-428.
49. Yamanaka, K., A. Ishihama, and K. Nagata. 1990. Reconstitution of influenza virus RNA-nucleoprotein complexes structurally resembling native viral ribonucleoprotein cores. J. Biol. Chem. 265:11151-11155.
50. Yamasaki, R. B., A. Vega, and R. E. Feeney. 1980. Modification of available arginine residues in proteins by p-hydroxyphenylglyoxal. Anal. Biochem. 109: 32-40.