An efficient strategy for validation of a point mutation associated with acetylcholinesterase sensitivity to azinphosmethyl in Colorado potato beetle

Pesticide Biochemistry and Physiology

Volumn 65, Issue 1, 1999, Pages 25-35

  Zhang, Aiguo ^a   Dunn, Jessica B ^a   Clark, J Marshall ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Acetylcholinesterase; Colorado potato beetle; cPASA; Insecticide resistance; Minisequencing reaction; Point mutation; Resistance monitoring; SSCP

Indexed keywords

ACETYLCHOLINESTERASE; AMINO ACID SEQUENCE; AZINPHOS METHYL; DIGOXIGENIN; ENZYME ACTIVITY; GENE SEQUENCING; INSECTICIDE RESISTANCE; POINT MUTATION; POLYMERASE CHAIN REACTION; SINGLE STRANDED CONFORMATIONAL POLYMORPHISM;

COLEOPTERA; HEXAPODA; INSECTA; LEPTINOTARSA DECEMLINEATA; SOLANUM TUBEROSUM;

EID: 0032851983     PISSN: 00483575     EISSN: None     Source Type: Journal    
DOI: 10.1006/pest.1999.2421     Document Type: Article

References (17)

1. J. A. Argentine, J. Marshall, Clark, and, D. N. Ferro, Genetics of azinphosmethyl and permethrin resistance in the Colorado potato beetle (Coleoptera: Chrysomelidae), J. Econ. Entomol. 82, 698.
2. Argentine J. A., Zhu K. Y., Lee S. H., Clark J. M. Biochemical mechanisms of azinphosmethyl resistance in isogenic strains of Colorado potato beetle. Pestic. Biochem. Physiol. 48:1994;63.
3. Zhu K. Y., Clark J. M. Purification and characterization of acetylcholinesterase from Colorado potato beetle Leptinotarsa decemlineata (Say). Insect Biochem. Mol. Biol. 24:1994;453.
4. Zhu K. Y., Clark J. M. Cloning and sequencing of cDNA encoding acetylcholinesterase in Colorado potato beetle, Leptinotarsa decemlineata (Say). Insect Biochem. Mol. Biol. 25:1995;1129.
5. Zhu K. Y., Lee S. H., Clark J. M. A point mutation of acetylcholinesterase associated with azinphosmethyl resistance and reduced fitness in Colorado potato beetle. Pestic. Biochem. Physiol. 55:1996.
6. Zhu K. Y., Clark J. M. Validation of a point mutation of acetylcholinesterase in Colorado potato beetle by polymerase chain reaction coupled to enzyme inhibition assay. Pestic. Biochem. Physiol. 57:1997;28.
7. Coustau C., ffrench-Constant R. Detection of cyclodiene insecticide resistance-associated mutations by single-stranded conformation polymorphism analysis. Pestic. Sci. 43:1995;267.
8. Kallio A. Detection of point mutations using the minisequencing method. Am. Biotech. Lab. Appl. Note. 15:1997;106.
9. Ellman G. L., Courtney K. D., Andres V. Jr., Featherstone R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7:1961;88.
10. Rappolee D. A., Mark D., Banda M. J., Werb Z. Wound macrophages express TGF-α and other growth factors in vivo: Analysis by mRNA phenotyping. Science. 241:1991;708.
11. Sarkar G., Cassady J., Bottema C. D. K., Sommer S. S. Characterization of polymerase chain reaction amplification of specific alleles. Anal. Biochem. 186:1990;64.
12. Hongyo T., Buzard G. S., Calvert R. J., Weghorst C. M. 'Cold SSCP': A simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses. Nucleic Acids Res. 21:1993;3637.
13. M. Normura, Y. Kato, and, T. Miyata, The genotype and heredity of modified acetylcholinesterase of the green rice leafhopper (Nephotettix cincticeps Uhler), Pestic. Biochem. Physiol, in press.
14. Bloom H., Beier H., Gross H. S. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis. 8:1987;93.
15. Lee S. H., Dunn J. B., Clark J. M., Soderlund D. M. Molecular analysis of kdr-like resistance in a permethrin-resistance strain of Colorado potato beetle. Pestic. Biochem. Physiol. 63:1999;63.
16. Binder T., Berg T., Siegert W., Schmidt C. PCR-SSCP: Nonradioisotopic detection with biotinylated primers and streptavidin-alkaline phosphatase conjugate. BioTechniques. 18:1995;780.
17. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. Detection of polymorphisms of human DNA by gel electrophroesis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA. 86:1989;2766.