Inactivation of the gene (cpe) encoding Clostridium perfringens enterotoxin eliminates the ability of two cpe-positive C. perfringens type A human gastrointestinal disease isolates to affect rabbit ileal loops

Molecular Microbiology

Volumn 33, Issue 5, 1999, Pages 946-958

  Sarker, Mahfuzur R ^a   Carman, Robert J ^b   McClane, Bruce A ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b TECHLAB INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENTEROTOXIN; VIRULENCE FACTOR;

ALLELE; ANIMAL EXPERIMENT; ARTICLE; BACTERIUM MUTANT; CLOSTRIDIUM PERFRINGENS; DIARRHEA; FOOD POISONING; GASTROINTESTINAL DISEASE; GENE EXPRESSION; GENE INACTIVATION; ILEUM; IMMUNOBLOTTING; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; RABBIT;

ANIMALS; BACTERIAL TOXINS; BLOTTING, SOUTHERN; CALCIUM-BINDING PROTEINS; CLOSTRIDIUM PERFRINGENS; ENTEROTOXINS; FEMALE; FOOD POISONING; GASTROINTESTINAL DISEASES; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; HUMANS; ILEUM; MALE; MUTATION; PHOSPHOLIPASE C; POLYMERASE CHAIN REACTION; RABBITS; SPECIES SPECIFICITY; VIRULENCE;

EID: 0032851049     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01534.x     Document Type: Article

References (29)

1. Awad, M.M., Bryant, A.E., Stevens, D.L., and Rood, J.I. (1995) Virulence studies on chromosomal α toxin and θ toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of a toxin in Clostridium perfringens-mediated gas gangrene. Mol Microbiol 15: 191-202.
2. Batholomew, B.A., Stringer, M.F., Watson, G.N., and Gilbert, R.J. (1985) Development and application of an enzyme-linked immunosorbent assay for Clostridium perfringens type A enterotoxin. J Clin Pathol 38: 222-228.
3. Bean, N.H., Goulding, J.S., Lao, C., and Angulo, F.J. (1996) Surveillance for foodborne-disease outbreaks - United States, 1988-82. Morbid Mortal Weekly Reports 45: 1-54.
4. Birkhead, G., Vogt, R.L., Heun, E.M., Snyder, J.T., and McClane, B.A. (1988) Characterization of an outbreak of Clostridium perfringens food poisoning by quantitative fecal culture and fecal enterotoxin measurement. J Clin Microbiol 26: 471-474.
5. Carman, R.J. (1997) Clostridium perfringens in spontaneous and antibiotic-associated diarrhoea of man and other animals. Rev Med Microbiol 8 (Suppl. 1): S43-S45.
6. Collie, R.E., and McClane, B.A. (1998) Evidence that the enterotoxin gene can be episomal in Clostridium perfringens isolates associated with nonfoodborne human gastrointestinal diseases. J Clin Microbiol 36: 30-36.
7. Collie, R.E., Kokai-Kun, J.F., and McClane, B.A. (1998) Phenotypic characterization of enterotoxigenic Clostridium perfringens isolates from non-foodborne human gastrointestinal diseases. Anaerobe 4: 69-79.
8. Czeczulin, J.R., Hanna, P.C., and McClane, B.A. (1993) Cloning, nucleotide sequencing, and expression of the Clostridium perfringens enterotoxin gene in Escherichia coli. Infect Immun 61: 3429-3439.
9. Czeczulin, J.R., Collie, R.E., and McClane, B.A. (1996) Regulated expression of Clostridium perfringens enterotoxin in naturally cpe-negative type A, B, and C isolates of C. perfringens. Infect Immun 64: 3301-3309.
10. Duncan, C.L., and Strong, D.H. (1969) Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens. J Bacteriol 100: 86-94.
11. Duncan, C.L., Sugiyama, H., and Strong, D.H. (1968) Rabbit ileal loop response to strains of Clostridium perfringens. J Bacteriol 95: 1560-1566.
12. Gibert, M., Jolivet-Reynaud, C., and Popoff, M.R. (1997) Beta2 toxin, a novel toxin produced by Clostridium perfringens. Gene 203: 65-73.
13. Ginter, A., Williamson, E.D., Dessy, F., Coppe, P., Bullifent, H., Howells, A., et al. (1996) Molecular variation between the alpha-toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animals. Microbiology 142: 191-198.
14. Hansen, M.V., and Elliott, L.P. (1980) New presumptive identification test for Clostridium perfringens: reverse CAMP test. J Clin Microbiol 12: 617-619.
15. Hatheway, C. (1990) Toxigenic clostridia. Clin Microb Rev 3: 66-76.
16. Kokai-Kun, J.F., Songer, J.G., Czeczulin, J.R., Chen, F., and McClane, B.A. (1994) Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens. J Clin Microbiol 32: 2533-2539.
17. McClane, B.A. (1997) Clostridium perfringens. In Food Microbiology: Fundamentals and Frontiers. Doyle, M.P., Beuchat, L.R., and Montville, T.J. (eds). Washington, DC: American Society for Microbiology Press, pp. 305-326.
18. McDonel, J.L. (1986) Toxins of Clostridium perfringens types A, B, C, D, and E. In Pharmacology of Bacterial Toxins. Dorner, F., and Drews, H. (eds). Oxford: Pergamon Press, pp. 477-517.
19. McDonel, J.L., and Duncan, C.L. (1975) Histopathological effect of Clostridium perfringens enterotoxin in the rabbit ileum. Infect Immun 12: 1214-1218.
20. McDonel, J.L., and Duncan, C.L. (1977) Regional localization of activity of Clostridium perfringens type A enterotoxin in the rabbit ileum, jejunum and duodenum. J Infect Dis 136: 661-666.
21. Niilo, L. (1970) Mechanism of action of the enteropathogenic factor of Clostridium perfringens. Infect Immun 3: 100-106.
22. Rood, J.I. (1998) Virulence genes of Clostridium perfringens. Annu Rev Microbiol 52: 333-360.
23. Sambrook, J.E., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
24. Sherman, S., Klein, E., and McClane, B.A. (1994) Clostridium perfringens type A enterotoxin induces concurrent development of tissue damage and fluid accumulation in the rabbit ileum. J Diarrheal Dis Res 12: 200-207.
25. Skjelkvale, R., and Uemura, T. (1977) Experimental diarrhea in human volunteers following oral administration of Clostridium perfringens enterotoxin. J Appl Bacteriol 46: 281-286.
26. Sloan, J., Warner, T.A., Scott, P.T., Bannam, T.L., Berryman, D.I., and Rood, J.I. (1992) Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid. Plasmid 27: 207-219.
27. Songer, J.G. (1996) Clostridial enteric diseases of domestic animals. Clin Microbiol Rev 9: 216-234.
28. Songer, J.G., and Meer, R.M. (1996) Genotyping of Clostridium perfringens by polymerase chain reaction is a useful adjunct to diagnosis of clostridial enteric disease in animals. Anaerobe 2: 197-203.
29. Zhao, Y., and Melville, S.B. (1998) Identification and characterization of sporulation-dependent promoters upstream of the enterotoxin gene (cpe) of Clostridium perfringens. J Bacteriol 180: 136-142.