Purification and characterization of the hepatic CYP2C and 3A isozymes from phenobarbitone pretreated rhesus monkey

Molecular and Cellular Biochemistry

Volumn 198, Issue 1-2, 1999, Pages 79-88

  Ramana, Kota V ^a   Kohli, Krishan K ^a  

^a POSTGRADUATE INSTITUTE OF MEDICAL EDUCATION AND RESEARCH   (India)

Author keywords

CYP2C; CYP3A; Cytochrome P450; Phenobarbitone; Rhesus monkey

Indexed keywords

AMINOPHENAZONE; ANTIBODY; CYTOCHROME B5; CYTOCHROME P450; ERYTHROMYCIN; ETHYLMORPHINE N DEMETHYLASE; HEME; ISOENZYME; LIPID; LIVER ENZYME; PHENOBARBITAL; PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; ENZYME METABOLISM; ENZYME PURIFICATION; HUMAN; MALE; MICROSOME; MOLECULAR WEIGHT; NONHUMAN; REACTION ANALYSIS; RHESUS MONKEY;

AMINO ACID SEQUENCE; ANIMALS; ARYL HYDROCARBON HYDROXYLASES; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; CYTOCHROME P-450 CYP3A; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MACACA MULATTA; MALE; MICROSOMES, LIVER; OXIDOREDUCTASES, N-DEMETHYLATING; PEPTIDE MAPPING; PHENOBARBITAL; SPECTRUM ANALYSIS;

EID: 0032849211     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006902212598     Document Type: Article

References (42)

1. Nelson DR, Koymans L, Kamataki T, Stegeman JJ, Feyereisen R, Waxman DJ, Waterman MR, Gotoh O, Coon MJ, Estabrook RW, Gunsalus IC, Nebert DW: P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6: 1-42, 1996
2. Palmour RM, Mulligan J, Howbert JJ, Ervin F: Of monkeys and men: Vervets and the genetics of human-like behaviors. Am J Hum Genet 61: 481-488, 1997
3. Dalet-Beluche I, Boulenc X, Fabre G, Maurel P, Bonfils C: Purification of two cytochrome P450 isozymes related to CYP2A and CYP3A gene families from monkey (baboon, Papio papio) liver microsomes. Eur J Biochem 204: 641-648, 1992
4. Ohmachi T, Fujii H, Sagami I, Suzaki Y, Fujiwara T, Watanabe M: Purification and characterization of cytochrome P450 from liver microsomes of 3-methylcholanthrene treated crab eating monkeys. J Biochem 105: 307-311, 1989
5. Ohmori S, Horie T, Guengerich FP, Kiuchi M, Kitada M: Purification and characterization of two forms of hepatic microsomal cytochrome P450 from untreated cynomolgus monkeys. Arch Biochem Biophys 305: 405-413, 1993
6. Ohmori S, Chiba K, Nasaka H, Horie T, Kitada M: Characterization of monkey cytochrome P450, P450 CMLd responsible for S-mephenytoin 4′-hydroxylation in hepatic microsomes of cynomolgus monkeys. Arch Biochem Biophys 311: 395-401, 1994
7. 14C-2,3,7,8-tetrachlorodibenzo-p-dioxin treated marmoset monkeys: Identification of the major form as a orthologue of P450 1A2. Biochim Biophys Acta 1200: 7-10, 1994
8. Kastner M, Schulz-Schalge T, Neubert D: Purification and properties of cytochrome P450 from liver microsomes of phenobarbital treated marmoset monkeys. Toxicol Lett 45: 261-270, 1989
9. Ohta K, Kitada M, Hashizume T, Komori M, Ohi H, Kamataki T: Purification of cytochrome P-450 from polychlorinated biphenyl-treated crab eating monkeys: High homology to a form of human cytochrome P-450. Biochim Biophys Acta 996: 142-145, 1989
10. Ohi H, Toratani S, Komori M, Miura T, Kitada M, Kamataki T: Comparative study of cytochrome P-450 in liver microsomes: A form of monkey cytochrome P-450, P450-MK1, immunochemically cross-reactive with antibodies to rat P-450-male. Biochem Pharmacol 38: 361-365, 1989
11. Bullock P, Pearce R, Draper A, Podval J, Bracken W, Thomas P, Parkinson A: Induction of liver microsomal cytochrome P450 in cynomolgus monkeys. Drug Metab Dispos 23: 736-748, 1995
12. Ojha V, Kohli KK: Induction of hepatic microsomal cytochrome P450 by dexamethasone in rhesus monkey (Macaca mulatto). Biochem Mol Biol Int 33: 1135-1143, 1994
13. Puri S, Kohli KK: Differences in hepatic drug metabolizing enzymes and their response to lindane in rat, rabbit and monkey. Pharmacol Toxicol 77: 136-141, 1995
14. Ramana KV, Kohli KK: Induction of hepatic cytochrome P450 from phenobarbitone pretreated rhesus monkey. Drug Chem Toxicol 19: 97-107, 1996
15. Elshourbagy NA, Guzelian PS: Separation, purification and characterization of a novel form of hepatic cytochrome P450 from rats treated with pregnenolone-16α-carbonitrile. J Biol Chem 255: 1279-1285, 1980
16. Oguri K, Kaneko H, Tanimoto Y, Yamada H, Yoshimura H: A constitutive form of guinea pig liver cytochrome P450 closely related to phenobarbital-inducible P450 (b/e). Arch Biochem Biophys 287: 105-111, 1991
17. Omura T, Sato R: The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239: 2370-2378, 1964
18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with Polin phenol reagent. J Biol Chem 193: 265-275, 1951
19. Lu AYH, Levin W: Partial purification of cytochrome P-450 and P-448 from rat liver microsomes. Biochem Biophys Res Commun 46: 1334-1339, 1972
20. Yasukochi Y, Masters BSS: Some properties of a detergent solubilized NADPH-cytochrome c (cytochrome P-450) reductase purified by biospecific affinity chromatography. J Biol Chem 251: 5337-5344, 1976.
21. 4. Nature 227: 680-685, 1970
22. Cleveland DW, Fisher SG, Krischner MW, Laemmli UK: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252: 1102-1106, 1977
23. Merril CR, Goldman D, Sedman SA, Ebert MH: Ultrasensitive stain for proteins in polyacrylamide gels shows regional variations in cerebrospinal fluid proteins. Science 211: 1437-1438, 1981
24. Kuwahara S, Harada N, Yoshioka H, Miyata T, Omura T: Purification and characterization of four forms of cytochrome P450 from liver microsomes of phenobarbital treated and 3-methylcholanthrene treated rats. J Biochem 95: 703-714, 1984
25. Nash T: The colorimetric estimation of formaldehyde by means of Hantzch reaction. Biochem J 55: 416-421, 1953
26. Matsudaira P: Sequence from picomole quantities of proteins electroblotted on topolyvinylidene difluoride membranes. J Biol Chem 262: 10035-10042, 1987
27. Edman P, Begg G: A protein sequenator. Eur J Biochem 1: 80-91, 1967
28. Lu AYH, Strobel HW, Coon MJ: Hydroxylation of benzphetamine and other drugs by a solubilized form of cytochrome P450 from liver microsomes: Lipid requirement for drug demethylation. Biochem Biophys Res Commun 36: 545-551, 1969
29. Puri V, Kohli KK: Purification and characterization of dexamethasone inducible hepatic cytochrome P450 isozymes from rhesus monkey. Drug Chem Toxicol 20: 11-19, 1997
30. West SB, Huang MT, Miwa GT, Lu AYH: Simple and rapid procedure for the purification of phenobarbital inducible cytochrome P450 from rat liver microsomes. Arch Biochem Biophys 193: 42-50, 1979
31. Jansson I, Mole JE, Schenkman JB: The isolation and comparison of multiple forms of CYP2B from untreated and phenobarbital treated rabbit liver microsomes. Arch Biochem Biophys 316: 275-284, 1995
32. Thomas PE, Bandiera S, Reik LM, Maines SL, Ryan DE, Levin W: Polyclonal and monoclonal antibodies as probes of rat hepatic cytochrome P-450 isozymes. Fed Proc 46: 2563-2566, 1987
33. Edwards RJ, Murray BP, Murray S, Schulz T, Neubert D, Gant TW, Thorgeirsson SS, Boobis AR, Davies DS: Contribution of CYP1A1 and CYP1A2 to the activation of heterocyclic amines in monkeys and human. Carcinogenesis 15: 829-836, 1994
34. Gillam EMJ, Baba T, Kim BR, Ohmori S, Guengerich FP: Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch Biochem Biophys 305: 123-131, 1993
35. 5. J Biol Chem 270: 24707-24718, 1995
36. Denison MS, Whitelock JP Jr: Xenobiotic-inducible transcription of cytochrome P450 genes. J Biol Chem 270: 18175-18178, 1995
37. Romkes M, Faletto MB, Blaisdell JA, Raucy JL, Goldstein JA: Cloning and expression of complementary DNAs for multiple members of the human cytochrome P450IIC subfamily. Biochemistry 30: 3247-3255, 1991
38. Umeno M, Morris S, Matsunaga E, Gelboin HV, Gonzalez FJ: Sequence of the 5'-end of the developmentally regulated rat P450PB1 (P450IIC6) gene. Nucleic Acids Res 16: 6249, 1988
39. Yoshioka H, Morohashi K, Sogawa K, Miyata T, Kawajiri K, Inayama S, Fujii-Kuriyama Y, Omura T: Structural analysis and specific expression of microsomal cytochrome P450(M1) mRNA in male rat livers. J Biol Chem 262: 1706-1711, 1987
40. Beaune PH, Umbenhauer DR, Bork RW, Lloyd RS, Guengerich FP: Isolation and sequence determination of a cDNA clone related to human cytochrome P-450 nifedipine oxidase. Proc Natl Acad Sci USA 83: 8064-8068, 1986
41. Aoyama T, Yamano S, Waxman DJ, Lapenson DP, Meyer UA, Fischer V, Tyndale R, Inaba T, Kalow W, Gelboin HV, Gonzalez FJ: Cytochrome P-450 hPCN3, anovel cytochrome P450IIIA gene product that is differentially expressed in adult human liver. cDNA sequence and deduced amino acid sequence and distinct specificities of cDNA expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine. J Biol Chem 264: 10388-10395, 1989
42. Gonzalez FJ, Nebert DW, Hardwick JP, Kasper CB: Complete cDNA and protein sequence of a pregnenolone 16α-carbonitrile induced cytochrome P450: A representative of a new gene family. J Biol Chem 260: 7435-7441, 1985