A new combined Bodian-Luxol technique for staining unmyelinated axons in semithin, resin-embedded peripheral nerves: A comparison with electron microscopy

Acta Neuropathologica

Volumn 98, Issue 4, 1999, Pages 323-329

  Deprez, M ^a   Ceuterick De Groote, C ^b   Fumal, A ^a   Reznik, M ^a   Martin, J J ^b  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

Electron microscopy; Histochemistry; Peripheral nerve; Unmyelinated fibers

Indexed keywords

RESIN;

ADULT; AGED; ARTICLE; CASE REPORT; CELL SIZE; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; FEMALE; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; MALE; MICROSCOPY; NERVE FIBER DEGENERATION; NONMYELINATED NERVE; OPTICAL DENSITY; PERIPHERAL NERVE; PRIORITY JOURNAL; STAINING; TISSUE PREPARATION; TISSUE SECTION;

AXONS; CELL COUNT; COLORING AGENTS; HUMANS; INDOLES; MALE; MICROSCOPY, ELECTRON; MIDDLE AGED; NERVE FIBERS; NERVE FIBERS, MYELINATED; PERIPHERAL NERVES; RESINS, PLANT; SILVER STAINING; SKIN; TISSUE EMBEDDING;

EID: 0032848191     PISSN: 00016322     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004010051088     Document Type: Article

References (21)

1. Bartko JJ (1966) The intraclass correlation coefficient as a measure of reliability. Psychol Rep 19:3-11
2. Behse F, Buchtal F, Carlsen F, Knappeis GG (1974) Endoneurial space and its constitutents in the sural nerve of patients with neuropathy. Brain 97:773-774
3. Behse F, Buchtal F, Carlsen F, Knappeis GG (1975) Unmyelinated fibers and Schwann cells of sural nerve in neuropathy. Brain 98:493-510
4. Gibbels E (1989) Morphometry of unmyelinated fibers. Clin Neuropathol 8:179-184
5. Gibbels E, Kentenich M (1990)Unmyelinated fibers in sural nerve biopsies of chronic inflammatory demyelinating polyneuropathy. Acta Neuropathol 80:439-447
6. Hanyu N, Ikeda S, Nakadai A, Yanagisawa N, Powell HC (1989) Peripheral nerve pathological findings in familial amyloid polyneuropathy: a correlative study of proximal sciatic nerve and sural nerve lesions. Ann Neurol 25:340-350
7. Johnson PC, Beggs JL, Olafsen AG, Watkins CJ (1994) Unmyelinated nerve fiber estimation by immunocytochemistry. Correlation with electron microscopy. J Neuropathol Exp Neurol 53:176-183
8. Kanda T, Tsukagoshi H, Oda M, Miyamoto K, Tanabe H (1991) Morphological changes in unmyelinated nerve fibres in the sural nerve with age. Brain 114:585-599
9. Lane BP, Europa DL (1965) Differential staining of ultrathin section of Epon-embedded tissues for light microscopy. J Histochem Cytochem 13:579
10. Larsson L-I (1986) Plastic embedding technique. In: Larsson L-I (ed) Immunocytochemistry: theory and practice. CRC Press, Boca Raton, pp 61-67
11. Llewelyn JG, Gilbey SG, Thomas PK, King RHM, Muddle JR, Watkins PJ (1991) Sural nerve morphometry in diabetic autonomic and painful sensory neuropathy. Brain 114:867-892
12. Midroni G, Bilbao JM (1995) Quantitative technique. In: Midroni G, Bilbao JM (eds) Biopsy diagnosis of peripheral neuropathy. Butterworth-Heineman, Newton, pp 38-40
13. Ochoa J (1978) Recognition of unmyelinated fiber disease: morphologic criteria. Muscle Nerve 1:375-387
14. Ochoa J, Mair WG (1969) The normal sural nerve in man. I. Ultrastructure and numbers of fibres and cells. Acta Neuropathol (Berl) 13:197-216
15. Ochoa J, Mair WG (1969) The normal sural nerve in man. II. Changes in the axons and Schwann cells due to ageing. Acta Neuropathol (Berl) 13:217-239
16. Ranson SW (1911) Non-medullated nerve fibres in the spinal nerves. Am J Anat 12:67-87
17. Reznik M (1996) Structure et fonctions du système nerveux cutané. Pathol Biol (Paris) 44:831-837
18. Schröder JM, Gibbels E (1977) Marklose Nervenfasern im Senium und im Spätstadium der Thalidomid-Polyneuropathie: quantitativ-elektronmikroskopische Untersuchungen. Acta Neuropathol (Berl) 39:271-280
19. Stevens JC, Lofgren EP, Dyck PJ (1973) Histometric evaluation of branches of peroneal nerve: technique for combined biopsy of muscle nerve and cutaneous tissue. Brain Res 52: 37-59
20. Thomas PK, Ochoa J (1993) Clinical features and differential diagnosis. In: Dyck PJ, Thomas PK (eds) Peripheral neuropathy. Saunders, Philadelphia, pp 757-764
21. Thomas PK, Berthold C-H, Ochoa J (1993) Microscopic anatomy of the peripheral nervous system: nerve trunks and spinal roots. In: Dyck PJ, Thomas PK (eds) Peripheral neuropathy. Saunders, Philadelphia, pp 56-66, 71-72