Impact of delayed fixation on evaluation of cell proliferation in intracranial malignant tumors

Applied Immunohistochemistry

Volumn 7, Issue 3, 1999, Pages 209-213

  Di Tommaso, L ^a   Kapucuoglu, N ^b   Losi, L ^a   Trere D ^c   Eusebi, V ^a  

^a BELLARIA HOSPITAL   (Italy)

^b SB Ankara   (Turkey)

^c UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Fixation delay; Ki 67 labeling index; Mitotic count Mitotic index

Indexed keywords

KI 67 ANTIGEN; POLYCLONAL ANTIBODY;

ARTICLE; CELL PROLIFERATION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTRACRANIAL TUMOR; MALIGNANT NEOPLASTIC DISEASE; MITOSIS; PRIORITY JOURNAL; TISSUE FIXATION;

EID: 0032847250     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199909000-00006     Document Type: Article

References (32)

1. Akerman M, Brandt L, Johson A, Olsson H. Mitotic activity in non-Hodgkin's lymphoma: relation to the Kiel classification and to prognosis. Br J Cancer 1987;55:219-23.
2. Baak JPA. Mitosis counting in tumours. Hum Pathol 1990;21: 683-5.
3. Bergers E, Jannink I, Van Diest PI, et al. The influence of fixation delay on mitotic activity and flow cytometric cell cycle variables. Hum Pathol 1990;28:95-100.
4. Bloom HJG, Richardson WW. Histological grading and prognosis in breast cancer. Br J Cancer 1957;11:359-77.
5. Brown DC, Gatter KC. Monoclonal antibody Ki-67: its use in histopathology. Histopathology 1990;17:489-503.
6. Bullough WS. Mitotic activity in the tissue of dead mice, and in tissue kept in physiological salt solution. Exp Cell Res 1950;1:410-20.
7. Chi CH, Ruboi CA, Lagerlöf B. The frequency and distribution of mitotic figures in dysplasia and carcinoma in situ. Cancer 1977; 39:1218-23.
8. Coindre JM, Trojani M, Contesso G, et al. Reproducibility of a histopathological grading system for adult soft tissue sarcoma. Cancer 1986;58:306-9.
9. Cross SS, Start RD, Smith JHF. Does delay in fixation affect the number of mitotic figures in processed tissue? J Clin Pathol 1990; 43:597-9.
10. Donhuijsen K. Mitosis counts: reproducibility and significance in grading of malignancy. Hum Pathol 1986;17:1122-5.
11. Donhuijsen K, Schmidt U, Hirche H, van Beuningen D, Budach V. Changes in mitotic rate and cell cycle fractions caused by delayed fixation. Hum Pathol 1990;21:709-14.
12. Edwards JL, Donaldson JT. The time of fixation and the mitotic index. Am J Clin Pathol 1964;41:148-62.
13. Ellis PSJ, Whitehead R. Mitosis counting: a need for reappraisal. Hum Pathol 1981;12:3-4.
14. Elston CW, Ellis IO. Pathological prognostic factors in breast cancer: I. the value of histological grade in breast cancer, experience from a large study with long-term follow-up. Histopathology 1991; 19:403-10.
15. Evans N. Mitotic figures in malignant tumors as affected of time before fixation of tissues. Arch Pathol Lab Med 1926;1:894-8.
16. Gerdes J, Becker MHG, Key G, Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol 1992;168:85-6.
17. Gerdes J, Li L, Schlueter C, et al. Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am J Pathol 1991;138:867-73.
18. Graem N, Helweg-Larsen K. Mitotic activity and delay in fixation of tumour tissue. Acta Pathol Microbiol Scand (A) 1979;87:375-8.
19. Hall PA, Levison DA. Review: assessment of cell proliferation in histological material. J Clin Pathol 1990;43:184-92.
20. Hall PA, Levison DA, Woods AL, et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 1990;162:285-94.
21. Hofstadter F, Knüchel R, Rüschoff J. Cell proliferation assessment in oncology. Virchows Arch 1995;427:323-41.
22. Kempson RL. Mitosis counting II. Hum Pathol 1976;7:482-3.
23. Kleihues P, Burger PC, Scheitauer BW. Histologic typing of central nervous system: WHO. Berlin: Springer-Verlag, 1993.
24. Larsson LI. Tissue preparation methods for light microscopic immunohistochemistry. Appl Immunohistochem 1993;1:2-16.
25. Munnakata S, Hendricks JB. Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue. J Histochem Cytochem 1993;41:1241-6.
26. Myhre-Jensen O, Kaae S, Madsen EH, Sneppen O. Histopathological grading in soft tissue tumours. Acta Pathol Microbiol Immunol Scand (A) 1983;91:145-50.
27. Norris HJ. Mitosis counting III. Hum Pathol 1976;7:483-4.
28. Rose DSC, Maddox PH, Brown DC. Which proliferation markers for routine immunohistology? A comparison of five antibodies. J Clin Pathol 1994;47:1010-4.
29. Scully RE. Mitosis counting I. Hum Pathol 1976;7:481-2.
30. Silverberg SG. Reproducibility of the mitosis count in the histologic diagnosis of smooth muscle tumours of the uterus. Hum Pathol 1976;7:451-4.
31. Start RD, Flynn MS, Cross SS, Rogers K, Smith JHF. Is the grading of breast carcinomas affected by a delay in fixation? Virchows Arch (A) 1991;419:475-9.
32. Van Diest PJ, Bank JPA, Matze-Cok P, et al. The reproducibility of mitosis counting in 3469 breast cancer specimens: results from the Multicenter Morphometric Mammary Carcinoma Project. Hum Pathol 1992;23:603-7.