Reduced pH causes structural changes in the potent mitogenic toxin of Pasteurella multocida

FEMS Microbiology Letters

Volumn 180, Issue 1, 1999, Pages 15-20

  Smyth, Martin G ^a,d   Sumner, Ian G ^b   Lax, Alistair J ^c  

^a INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

^b INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^c GUY S HOSPITAL   (United Kingdom)

^d GLAXOSMITHKLINE   (United Kingdom)

Author keywords

CD; Circular dichroism; Low pH transition; Pasteurella multocida toxin; Refolding; Transverse urea gradient gels

Indexed keywords

BACTERIAL TOXIN; UREA;

ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; NONHUMAN; PASTEURELLA MULTOCIDA; PH; PRIORITY JOURNAL; PROTEIN FOLDING; TOXIN ANALYSIS; TOXIN STRUCTURE;

3T3 CELLS; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CIRCULAR DICHROISM; ELECTROPHORESIS, GEL, PULSED-FIELD; MICE; PROTEIN DENATURATION; PROTEIN FOLDING; UREA;

EID: 0032846834     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00456-5     Document Type: Article

References (19)

1. Rozengurt, E., Higgins, T., Chanter, N., Lax, A.J. and Staddon, J.M. (1990) Pasteurella multocida toxin: potent mitogen for cultured fibroblasts. Proc. Natl. Acad. Sci. USA 87, 123-127.
2. Murphy, A.C. and Rozengurt, E. (1992) Pasteurella multocida toxin selectively facilitates phosphatidylinositol 4, 5-bisphosphate hydrolysis by bombesin, vasopressin and endothelin: requirement for a functional G protein. J. Biol. Chem. 267, 25296-25303.
3. qα-coupled phospholipase C-β1. J. Biol. Chem. 272, 1268-1275.
4. FAK and paxillin tyrosine phosphorylation, actin stress fiber formation and focal contact assembly in Swiss 3T3 cells. J. Biol. Chem. 271, 439-445.
5. Smyth, M.G., Pickersgill, R.W. and Lax, A.J. (1995) The potent mitogen Pasteurella multocida toxin is highly resistant to proteolysis but becomes susceptible at lysosomal pH. FEBS Lett. 360, 62-66.
6. van der Goot, F.G., Lakey, J.H. and Pattus, F. (1992) The molten globule intermediate for protein insertion or translocation through membranes. Trends Cell Biol. 2, 343-348.
7. Falnes, P.Ø., Choe, S., Madshus, I.H., Wilson, B.A. and Olsnes, S. (1994) Inhibition of membrane translocation of diphtheria toxin A-fragment by internal disulfide bridges. J. Biol. Chem. 269, 8402-8407.
8. Goldenberg, D.P. and Creighton, T.E. (1984) Gel electrophoresis in studies of protein conformation and folding. Anal. Biochem. 138, 1-18.
9. Heukeshoven, J. and Dernick, R. (1985) Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103-112.
10. Ward, P.N., Higgins, T.E., Murphy, A.C., Mullan, P.B., Rozengurt, E. and Lax, A.J. (1994) Mutation of a putative ADP-ribosylation motif in the Pasteurella multocida toxin does not affect mitogenic activity. FEBS Lett. 342, 81-84.
11. Provencher, S.W. and Glockner, J. (1981) Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20, 33-37.
12. Privalov, P.L. (1982) Stability of proteins: proteins which do not present a single cooperative system. Adv. Protein Chem. 35, 1-104.
13. Choe, S., Bennett, M.J., Fujii, G., Curmi, P.M.G., Kantardjief, K.A., Collier, R.J. and Eisenberg, D. (1992) The crystal structure of diphtheria toxin. Nature 357, 216-222.
14. Falbo, V., Pace, T., Picci, L. and Caprioli, A. (1993) Isolation and nucleotide sequence of the gene encoding cytotoxin necrotizing factor 1 of Escherichia coli. Infect. Immun. 61, 4909-4914.
15. rho-dependent tyrosine phosphorylation of focal adhesion kinase and paxillin in Swiss 3T3 cells. J. Biol. Chem. 272, 9587-9596.
16. Lemichez, E., Flatau, G., Bruzzone, M., Boquet, P. and Gauthier, M. (1997) Molecular localization of the Escherichia coli cytotoxic necrotizing factor CNF1 cell-binding and catalytic domains. Mol. Microbiol. 24, 1061-1070.
17. Ward, P.N., Miles, A.J., Sumner, I.G., Thomas, L.H. and Lax, A.J. (1998) Activity of the mitogenic Pasteurella multocida toxin requires an essential C-terminal cysteine residue. Infect. Immun. 66, 5636-5642.
18. London, E. (1992) Diphtheria toxin: membrane interaction and membrane translocation. Biochim. Biophys. Acta 1113, 25-51.
19. Dibello, I.C., Poulain, B., Shone, C.C., Taue, L. and Dolly, J.O. (1994) Antagonism of the intracellular action of botulinum neurotoxin type-a with monoclonal-antibodies that map to light-chain epitopes. Eur. J. Biochem. 219, 161-169.